Anti-CDC7 抗体 [EPR4730] (ab108332)
![Western blot - Anti-CDC7 antibody [EPR4730] (ab108332)](https://www.abcam.co.jp/ps/products/108/ab108332/Images/ab108332-120907-anti-cdc7-antibody-epr4730-western-blot.jpg "Western blot - Anti-CDC7 antibody [EPR4730] (ab108332)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4730] to CDC7
- Suitable for: Flow Cyt (Intra), WB
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-CDC7 antibody [EPR4730]
CDC7 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR4730] to CDC7 -
由来種
Rabbit -
アプリケーション
適用あり: Flow Cyt (Intra), WBmore details
適用なし: ICC/IF,IHC-P or IP -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- Jurkat, HeLa, and PC3 cell lysates; Jurkat cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
精製度
Tissue culture supernatant -
ポリ/モノ
モノクローナル -
クローン名
EPR4730 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab108332の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Flow Cyt (Intra) |
1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
| WB |
1/1000 - 1/10000. Detects a band of approximately 64 kDa (predicted molecular weight: 64 kDa).
|
| 特記事項 |
|---|
|
Flow Cyt (Intra)
1/100 - 1/500. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB
1/1000 - 1/10000. Detects a band of approximately 64 kDa (predicted molecular weight: 64 kDa). |
ターゲット情報
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機能
Seems to phosphorylate critical substrates that regulate the G1/S phase transition and/or DNA replication. Can phosphorylates MCM2 and MCM3. -
配列類似性
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDC7 subfamily.
Contains 1 protein kinase domain. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 8317 Human
- Entrez Gene: 12545 Mouse
- Entrez Gene: 360908 Rat
- Omim: 603311 Human
- SwissProt: O00311 Human
- SwissProt: Q9Z0H0 Mouse
- Unigene: 533573 Human
- Unigene: 20842 Mouse
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別名
- Cdc 7 antibody
- CDC7 antibody
- CDC7 cell division cycle 7 antibody
see all
画像
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Western blot - Anti-CDC7 antibody [EPR4730] (ab108332)All lanes : Anti-CDC7 antibody [EPR4730] (ab108332) at 1/1000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : PC3 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 64 kDa -
![Flow Cytometry (Intracellular) - Anti-CDC7 antibody [EPR4730] (ab108332)](https://www.abcam.co.jp/ps/products/108/ab108332/Images/ab108332-120906-anti-cdc7-antibody-epr4730-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-CDC7 antibody [EPR4730] (ab108332)ab108332, at 1/100 dilution, staining CDC7 in permeabilized Jurkat cells by intracellular flow cytometry (red). Rabbit IgG (green).
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![Anti-CDC7 antibody [EPR4730] (ab108332)](https://www.abcam.co.jp/ps/products/108/ab108332/Images/ab108332-3-benefits-of-recombinant-antibodies.png)
Anti-CDC7 antibody [EPR4730] (ab108332)
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab108332 は 3 報の論文で使用されています。
- Li Q et al. CDC7-dependent transcriptional regulation of RAD54L is essential for tumorigenicity and radio-resistance of glioblastoma. Transl Oncol 11:300-306 (2018). PubMed: 29413763
- Pérez-Olivares M et al. Functional interplay between c-Myc and Max in B lymphocyte differentiation. EMBO Rep 19:N/A (2018). PubMed: 30126925
- Zhen H et al. Toona Sinensis and Moschus Decoction Induced Cell Cycle Arrest in Human Cervical Carcinoma HeLa Cells. Evid Based Complement Alternat Med 2014:121276 (2014). WB ; Human . PubMed: 24511319