Anti-Cdc6 (phospho S54) 抗体 [EPR759Y] (ab75809)
![Western blot - Anti-Cdc6 (phospho S54) antibody [EPR759Y] (ab75809)](https://www.abcam.co.jp/ps/products/75/ab75809/Images/ab75809-280679-anti-cdc6-phospho-s54-antibody-epr759y-western-blot.jpg "Western blot - Anti-Cdc6 (phospho S54) antibody [EPR759Y] (ab75809)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR759Y] to Cdc6 (phospho S54)
- Suitable for: WB, ICC/IF, Dot blot
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Cdc6 (phospho S54) antibody [EPR759Y]
Cdc6 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR759Y] to Cdc6 (phospho S54) -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IF, Dot blotmore details
適用なし: Flow Cyt,IHC-P or IP -
種交差性
交差種: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- WB: Raji + FBS cell lysates. ICC: HeLa cells. Dot Blot: Cdc6 (pS54) phospho peptide.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR759Y -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab75809の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000 - 1/2000. Detects a band of approximately 63 kDa (predicted molecular weight: 63 kDa).
|
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| ICC/IF |
1/100 - 1/250.
|
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| Dot blot |
1/1000.
|
| 特記事項 |
|---|
|
WB
1/1000 - 1/2000. Detects a band of approximately 63 kDa (predicted molecular weight: 63 kDa). |
|
ICC/IF
1/100 - 1/250. |
|
Dot blot
1/1000. |
ターゲット情報
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機能
Involved in the initiation of DNA replication. Also participates in checkpoint controls that ensure DNA replication is completed before mitosis is initiated. -
配列類似性
Belongs to the CDC6/cdc18 family. -
細胞内局在
Nucleus. Cytoplasm. The protein is nuclear in G1 and cytoplasmic in S-phase cells. - Information by UniProt
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参照データベース
- Entrez Gene: 990 Human
- Omim: 602627 Human
- SwissProt: Q99741 Human
- Unigene: 405958 Human
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別名
- Cdc 18L antibody
- Cdc 6 antibody
- CDC18 (cell division cycle 18, S.pombe, homolog) like antibody
see all
画像
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Western blot - Anti-Cdc6 (phospho S54) antibody [EPR759Y] (ab75809)All lanes : Anti-Cdc6 (phospho S54) antibody [EPR759Y] (ab75809) at 1/5000 dilution
Lane 1 : Hela (Human cervix adenocarcinoma epithelial cell) whole cell lysates
Lane 2 : Hela (Human cervix adenocarcinoma epithelial cell) treated with Hydroxyurea (3mM, 20 h). Whole cell lysates
Lane 3 : Hela (Human cervix adenocarcinoma epithelial cell) treated with Hydroxyurea (3mM, 20 h). Whole cell lysates.Then the membrane was incubated with phosphatase.
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 63 kDa
Exposure time: 30 secondsBlocking buffer and diluting buffer: 5% NFDM/TBST
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![Dot Blot - Anti-Cdc6 (phospho S54) antibody [EPR759Y] (ab75809)](https://www.abcam.co.jp/ps/products/75/ab75809/Images/ab75809-280685-anti-cdc6-phospho-s54-antibody-epr759y-dot-blot.jpg)
Dot Blot - Anti-Cdc6 (phospho S54) antibody [EPR759Y] (ab75809)Primary antibody dilution 1:1000 dilution. Secondary antibody used was Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051). Secondary ab dilution 1:100,000 dilution. Blocking buffer and concentration 5% NFDM/TBST. Diluting buffer and concentration 5% NFDM /TBST. Lane 1:Cdc6 (pS54) phospho peptide, Lane 2:Cdc6 non-phospho peptide. Exposure time 3 minutes
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![Immunocytochemistry/ Immunofluorescence - Anti-Cdc6 (phospho S54) antibody [EPR759Y] (ab75809)](https://www.abcam.co.jp/ps/products/75/ab75809/Images/ab75809-280682-anti-cdc6-phospho-s54-antibody-epr759y-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Cdc6 (phospho S54) antibody [EPR759Y] (ab75809)Cell line 293T (Human embryonic kidney epithelial cell). Target AbID Ab75809 anti- Cdc6 (phospho S54)
ab150077 AlexaFluor®488 Goat anti-Rabbit secondary. Counterstain AbID Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594). Fixative 4% Paraformaldehyde. Permeabilisation 0.1% tritonX-100. Nuclear counter stain DAPI. Datasheet Comments Confocal image showing the expression was increased after treatment with Hydroxyurea (3mM 20hour), the signal was decreased after treatment with the Lambda Protein Phosphatase 31℃ for 2h. Target 1oAb dilution 1:100 2.4 μg/ml, Target 2ndry Ab dilution 1:1000 2 μg/ml, Counterstain Ab dilution 1:200 2.5 μg/ml
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![Western blot - Anti-Cdc6 (phospho S54) antibody [EPR759Y] (ab75809)](https://www.abcam.co.jp/ps/products/75/ab75809/Images/ab75809-90896-anti-cdc6-phospho-s54-antibody-epr759y-western-blot.jpg)
Western blot - Anti-Cdc6 (phospho S54) antibody [EPR759Y] (ab75809)All lanes : Anti-Cdc6 (phospho S54) antibody [EPR759Y] (ab75809) at 1/2000 dilution
Lane 1 : Raji cell lysate
Lane 2 : Raji +
FBS cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 63 kDa
Observed band size: 63 kDa -
![Anti-Cdc6 (phospho S54) antibody [EPR759Y] (ab75809)](https://www.abcam.co.jp/ps/products/75/ab75809/Images/ab75809-6-benefits-of-recombinant-antibodies.png)
Anti-Cdc6 (phospho S54) antibody [EPR759Y] (ab75809)
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab75809 は 4 報の論文で使用されています。
- Zhang J et al. BRD4 facilitates replication stress-induced DNA damage response. Oncogene 37:3763-3777 (2018). PubMed: 29636547
- Müllers E et al. Residual Cdk1/2 activity after DNA damage promotes senescence. Aging Cell 16:575-584 (2017). PubMed: 28345297
- Karanika S et al. Targeting DNA Damage Response in Prostate Cancer by Inhibiting Androgen Receptor-CDC6-ATR-Chk1 Signaling. Cell Rep 18:1970-1981 (2017). PubMed: 28228262
- Fan X et al. Role of Cdc6 in re-replication in cells expressing human papillomavirus E7 oncogene. Carcinogenesis 37:799-809 (2016). PubMed: 27207654