Anti-Cdc6 抗体 [Cdc6 9H8/5] (ab20150)
Key features and details
- Mouse monoclonal [Cdc6 9H8/5] to Cdc6
- Suitable for: IP, WB
- Reacts with: Saccharomyces cerevisiae
- Isotype: IgG1
製品の概要
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製品名
Anti-Cdc6 antibody [Cdc6 9H8/5]
Cdc6 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [Cdc6 9H8/5] to Cdc6 -
由来種
Mouse -
アプリケーション
適用あり: IP, WBmore details -
種交差性
交差種: Saccharomyces cerevisiae -
免疫原
Full length protein corresponding to Saccharomyces cerevisiae Cdc6.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.02% Sodium azide
Constituent: 99.98% PBS -
Concentration information loading... -
精製度
Protein A/G purified -
ポリ/モノ
モノクローナル -
クローン名
Cdc6 9H8/5 -
ミエローマ
unknown -
アイソタイプ
IgG1 -
軽鎖の種類
unknown -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab20150の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IP |
Use at an assay dependent concentration.
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| WB |
Use at an assay dependent concentration.
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| 特記事項 |
|---|
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IP
Use at an assay dependent concentration. |
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WB
Use at an assay dependent concentration. |
ターゲット情報
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機能
Involved in the initiation of DNA replication. Also participates in checkpoint controls that ensure DNA replication is completed before mitosis is initiated. -
配列類似性
Belongs to the CDC6/cdc18 family. -
細胞内局在
Nucleus. Cytoplasm. The protein is nuclear in G1 and cytoplasmic in S-phase cells. - Information by UniProt
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別名
- Cdc 18L antibody
- Cdc 6 antibody
- CDC18 (cell division cycle 18, S.pombe, homolog) like antibody
see all
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (7)
ab20150 は 7 報の論文で使用されています。
- Scherr MJ et al. Mobile origin-licensing factors confer resistance to conflicts with RNA polymerase. Cell Rep 38:110531 (2022). PubMed: 35320708
- Evrin C et al. In the absence of ATPase activity, pre-RC formation is blocked prior to MCM2-7 hexamer dimerization. Nucleic Acids Res 41:3162-72 (2013). WB . PubMed: 23376927
- Evrin C et al. The ORC/Cdc6/MCM2-7 complex facilitates MCM2-7 dimerization during prereplicative complex formation. Nucleic Acids Res N/A:N/A (2013). IP . PubMed: 24234446
- Ikui AE et al. A yeast GSK-3 kinase Mck1 promotes Cdc6 degradation to inhibit DNA re-replication. PLoS Genet 8:e1003099 (2012). WB . PubMed: 23236290
- Zhang JY et al. Tumor necrosis factor receptor 1/c-Jun-NH2-kinase signaling promotes human neoplasia. Cancer Res 67:3827-34 (2007). WB . PubMed: 17440097
- Perkins G et al. Separate SCF(CDC4) recognition elements target Cdc6 for proteolysis in S phase and mitosis. EMBO J 20:4836-45 (2001). PubMed: 11532947
- Donovan S et al. Cdc6p-dependent loading of Mcm proteins onto pre-replicative chromatin in budding yeast. Proc Natl Acad Sci U S A 94:5611-6 (1997). PubMed: 9159120