Anti-CDC45L 抗体 [EPR5758] (ab108350)
![Western blot - Anti-CDC45L antibody [EPR5758] (ab108350)](https://www.abcam.co.jp/ps/products/108/ab108350/Images/CDC45L-Primary-antibodies-ab108350-1.jpg "Western blot - Anti-CDC45L antibody [EPR5758] (ab108350)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5758] to CDC45L
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-CDC45L antibody [EPR5758]
CDC45L 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR5758] to CDC45L -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details
適用なし: IP -
種交差性
交差種: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- HeLa, Jurkat, K562, and 293T cell lysates. Human kidney tissue
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
解離定数（KD 値）
KD = 3.17 x 10 -11 M Learn more about KD -
バッファー
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
精製度
Tissue culture supernatant -
ポリ/モノ
モノクローナル -
クローン名
EPR5758 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab108350の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/10000 - 1/50000. Detects a band of approximately 65 kDa (predicted molecular weight: 66 kDa).
|
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| IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Perform antigen retrieval before commencing with IHC staining protocol |
| 特記事項 |
|---|
|
WB
1/10000 - 1/50000. Detects a band of approximately 65 kDa (predicted molecular weight: 66 kDa). |
|
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Perform antigen retrieval before commencing with IHC staining protocol |
ターゲット情報
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機能
Required for initiation of chromosomal DNA replication. -
組織特異性
Widely expressed, highest levels are found in adult testis and thymus and in fetal liver. -
配列類似性
Belongs to the CDC45 family. -
発生段階
Transcript peaks at G1-S transition, but total protein remains constant throughout the cell cycle. Expressed in multiple tissues during embryogenesis, including neural crest-derived structures. -
細胞内局在
Cytoplasm. Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 8318 Human
- Omim: 603465 Human
- SwissProt: O75419 Human
- Unigene: 474217 Human
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別名
- CDC 45 antibody
- CDC 45L antibody
- CDC45 (cell division cycle 45, S.cerevisiae homolog) like antibody
see all
画像
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Western blot - Anti-CDC45L antibody [EPR5758] (ab108350)All lanes : Anti-CDC45L antibody [EPR5758] (ab108350) at 1/10000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : K562 cell lysate
Lane 4 : 293T (Human embryonic kidney epithelial cell) cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 66 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDC45L antibody [EPR5758] (ab108350)](https://www.abcam.co.jp/ps/products/108/ab108350/Images/CDC45L-Primary-antibodies-ab108350-2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDC45L antibody [EPR5758] (ab108350)Immunohistochemical analysis of paraffin-embedded Human kidney tissue using ab108350 at 1/50
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![OI-RD Scanning - Anti-CDC45L antibody [EPR5758] (ab108350)](https://www.abcam.co.jp/ps/products/108/ab108350/Images/CDC45L-Primary-antibodies-ab108350-3.jpg)
OI-RD Scanning - Anti-CDC45L antibody [EPR5758] (ab108350) -
![Anti-CDC45L antibody [EPR5758] (ab108350)](https://www.abcam.co.jp/ps/products/108/ab108350/Images/ab108350-4-benefits-of-recombinant-antibodies.png)
Anti-CDC45L antibody [EPR5758] (ab108350)
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab108350 は 2 報の論文で使用されています。
- Zhang K et al. Demethylzeylasteral inhibits glioma growth by regulating the miR-30e-5p/MYBL2 axis. Cell Death Dis 9:1035 (2018). PubMed: 30305611
- Anantha RW et al. Requirement of heterogeneous nuclear ribonucleoprotein C for BRCA gene expression and homologous recombination. PLoS One 8:e61368 (2013). WB . PubMed: 23585894