AB2357

Anti-Cdc25A 抗体 [DCS-120 + DCS-121]

Name: Anti-Cdc25A 抗体 [DCS-120 + DCS-121] (ab2357) | Abcam
Brand: Abcam Limited
SKU: AB2357
Price: 49000 JPY
Availability: InStock
Rating: 2 (1 reviews)

Anti-Cdc25A antibody [DCS-120 + DCS-121]

(1 Review)

(15 Publications)

Mouse Multiclonal Cdc25A antibody. Suitable for IHC-P and reacts with Human samples. Cited in 15 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human M-phase inducer phosphatase 1.

別名を表示する

M-phase inducer phosphatase 1, Dual specificity phosphatase Cdc25A, CDC25A

1 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdc25A antibody [DCS-120 + DCS-121] (AB2357)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdc25A antibody [DCS-120 + DCS-121] (AB2357)

ab2357 staining human tonsil by IHC-P.

Key facts

宿主種

Mouse

クローン性

Multiclonal

クローン番号

DCS-120 + DCS-121

アイソタイプ

IgG2a

軽鎖のタイプ

unknown

キャリアフリー

交差種

Human

アプリケーション

IHC-P

applications

免疫原

Recombinant Full Length Protein corresponding to Human M-phase inducer phosphatase 1.

P30304

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p>ABC method.</p> Perform heat-mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol." } } }

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製品の詳細

What is an oligoclonal antibody?
This oligoclonal antibody is a cocktail of monoclonal antibodies that have been carefully selected. Oligoclonal antibodies have not only the specificity and batch-to-batch consistency of a monoclonal antibody, but also have the advantage of the sensitivity of a polyclonal antibody due to their ability to recognize multiple epitopes on an antigen.

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出荷温度及び保存条件

製品の状態

Liquid

精製度

IgG fraction

バッファー組成

pH: 7.3 - 7.5 Preservative: 0.05% Sodium azide Constituents: 1% BSA

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Cdc25A also known as Cell Division Cycle 25A acts as a phosphatase that removes phosphate groups from cyclin-dependent kinases (CDKs). This process mainly activates CDKs which are important for the cell cycle progression. Cdc25A has a molecular mass of approximately 65 kDa. The protein expresses in various cell types but finds higher levels in proliferative tissues. Additionally it localizes primarily in the nucleus where it executes its function during specific cell cycle phases.

Biological function summary

Cdc25A plays a big role in controlling transitions between cell cycle phases particularly the G1 to S phase. It participates in a CDK complex which includes partners like Cyclin E and Cyclin A driving progression through cell cycle checkpoints. During DNA replication Cdc25A's activity ensures that cells duplicate accurately maintaining genome integrity and balance in cell proliferation.

Pathways

Cdc25A functions within the cell cycle regulation and DNA damage repair pathways. It interacts importantly with proteins like Checkpoint Kinase 1 and 2 (Chk1/Chk2) involved in responding to DNA damage. During adverse conditions Chk1/Chk2 phosphorylate Cdc25A leading to its degradation and preventing further cycle progression. This mechanism helps maintain genomic stability by halting the cycle until DNA repair occurs.

Cdc25A holds connections to cancer and neurodegenerative conditions. Its overexpression often links to cancers as uncontrolled activity allows for unscheduled cell proliferation. Similarly dysregulation of Cdc25A relates to proteins like p53 which suppress tumor growth. In neurodegenerative disorders Cdc25A's misregulation can also increase the potential for neuronal cell cycle re-entry contributing to neuronal death. Understanding these relationships is essential for developing therapeutic targets.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression (PubMed : 12676925, PubMed : 14559997, PubMed : 1836978, PubMed : 20360007). Directly dephosphorylates CDK1 and stimulates its kinase activity (PubMed : 20360007). Also dephosphorylates CDK2 in complex with cyclin-E, in vitro (PubMed : 20360007).

See full target information M-phase inducer phosphatase 1

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文献 (15)

Recent publications for all applications. Explore the full list and refine your search

PLoS biology 23:e3003096 PubMed40215234

2025

Interferon regulatory factor 4 mediates nonenzymatic IRE1 dependency in multiple myeloma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Ioanna Oikonomidi,Vasumathi Kameswaran,Victoria C Pham,Iratxe Zuazo-Gaztelu,Lauren M Gutgesell,Scot Marsters,Bence Daniel,Jennie R Lill,Zora Modrusan,Avi Ashkenazi

NPJ precision oncology 9:80 PubMed40119070

2025

ARRB2 promotes cervical cancer progression via stabilizing CDC25A mRNA through m6A-IGF2BP1-dependent manner.

Applications

Unspecified application

Species

Unspecified reactive species

Lijie Li,Jie Zeng,Mengying Liu,Hui Cheng,Yuyin He,Sili He,Chen Wang

Cell & bioscience 14:109 PubMed39210450

2024

MST1R-targeted therapy in the battle against gallbladder cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Wang,Chao Huang,Li Zhang,Liqin Yu,Yangming Liu,Puxiongzhi Wang,Rongmu Xia

International journal of biological sciences 19:258-280 PubMed36594088

2023

Validation of ESM1 Related to Ovarian Cancer and the Biological Function and Prognostic Significance.

Applications

Unspecified application

Species

Unspecified reactive species

Yu-Kun Li,Tian Zeng,Yang Guan,Jue Liu,Nian-Chun Liao,Meng-Jie Wang,Ke-Xin Chen,Xian-Yu Luo,Chang-Ye Chen,Fei-Fei Quan,Juan Wang,Qun-Feng Zhang,Juan Zou

Cell biochemistry and function 38:1139-1151 PubMed32869878

2020

LINC00662 contributes to the progression and the radioresistance of cervical cancer by regulating miR-497-5p and CDC25A.

Applications

Unspecified application

Species

Unspecified reactive species

Jiemei Wei,Lili Wang,Yanli Sun,Yongxin Bao

Bioscience reports 40: PubMed32091090

2020

Naringin protects endothelial cells from apoptosis and inflammation by regulating the Hippo-YAP Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Hui Zhao,Meirong Liu,Hui Liu,Rong Suo,Chengzhi Lu

Journal of Cancer 11:2158-2170 PubMed32127943

2020

B7-H3 promotes the cell cycle-mediated chemoresistance of colorectal cancer cells by regulating CDC25A.

Applications

Unspecified application

Species

Unspecified reactive species

Yanchao Ma,Ruoqin Wang,Huimin Lu,Xiaomi Li,Guangbo Zhang,Fengqing Fu,Lei Cao,Shenghua Zhan,Zhenxin Wang,Zhongbin Deng,Tongguo Shi,Xueguang Zhang,Weichang Chen

FEBS open bio 9:1869-1879 PubMed31505105

2019

miR-122-5p modulates the radiosensitivity of cervical cancer cells by regulating cell division cycle 25A (CDC25A).

Applications

Unspecified application

Species

Unspecified reactive species

Feng-Na Ding,Bao-Hong Gao,Xia Wu,Chun-Wu Gong,Wei-Qing Wang,Shu-Mao Zhang

International journal of molecular medicine 44:1991-2002 PubMed31573047

2019

miR‑19a promotes vascular smooth muscle cell proliferation, migration and invasion through regulation of Ras homolog family member B.

Applications

Unspecified application

Species

Unspecified reactive species

Gengxin Sun,Hui Song,Suya Wu

Pathology oncology research : POR 25:493-501 PubMed29532409

2018

Protein Expression Analysis in Uterine Cervical Cancer for Potential Targets in Treatment.

Applications

Unspecified application

Species

Unspecified reactive species

Sugela Blancas,Rogelio Medina-Berlanga,Liliana Ortíz-García,Alfredo Loredo-Ramírez,Leticia Santos

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Cdc25A 抗体 [DCS-120 + DCS-121]

Anti-Cdc25A antibody [DCS-120 + DCS-121]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdc25A antibody [DCS-120 + DCS-121] (AB2357)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

軽鎖のタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製度

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (15)

代替製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

組合せ製品

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Abcam product promise