Anti-Cdc25A 抗体 (ab989)
Key features and details
- Rabbit polyclonal to Cdc25A
- Suitable for: IP, WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Cdc25A antibody
Cdc25A 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Cdc25A -
由来種
Rabbit -
アプリケーション
適用あり: IP, WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Chimpanzee, Orangutan
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免疫原
Synthetic peptide (Human) conjugated to KLH - which represented a portion of human Cell Division Cycle 25a encoded by exon 7 (LocusLink ID 993).
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ポジティブ･コントロール
- WB: HeLa and HEK-293T whole cell lysates. IP: HEK-293T whole cell lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab989の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IP |
Use at 2-10 µg/mg of lysate.
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| WB | (1) |
1/1000 - 1/10000. Detects a band of approximately 65 kDa (predicted molecular weight: 59 kDa).
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| 特記事項 |
|---|
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IP
Use at 2-10 µg/mg of lysate. |
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WB
1/1000 - 1/10000. Detects a band of approximately 65 kDa (predicted molecular weight: 59 kDa). |
ターゲット情報
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機能
Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Directly dephosphorylates CDK1 and stimulates its kinase activity. Also dephosphorylates CDK2 in complex with cyclin E, in vitro. -
配列類似性
Belongs to the MPI phosphatase family.
Contains 1 rhodanese domain. -
ドメイン
The phosphodegron motif mediates interaction with specific F-box proteins when phosphorylated. Putative phosphorylation sites at Ser-79 and Ser-82 appear to be essential for this interaction. -
翻訳後修飾
Phosphorylated by CHEK1 on Ser-76, Ser-124, Ser-178, Ser-279, Ser-293 and Thr-507 during checkpoint mediated cell cycle arrest. Also phosphorylated by CHEK2 on Ser-124, Ser-279, and Ser-293 during checkpoint mediated cell cycle arrest. Phosphorylation on Ser-178 and Thr-507 creates binding sites for YWHAE/14-3-3 epsilon which inhibits CDC25A. Phosphorylation on Ser-76, Ser-124, Ser-178, Ser-279 and Ser-293 may also promote ubiquitin-dependent proteolysis of CDC25A by the SCF complex. Phosphorylation of CDC25A at Ser-76 by CHEK1 primes it for subsequent phosphorylation at Ser-79, Ser-82 and Ser-88 by NEK11. Phosphorylation by NEK11 is required for BTRC-mediated polyubiquitination and degradation. Phosphorylation by PIM1 leads to an increase in phosphatase activity. Phosphorylated by PLK3 following DNA damage, leading to promote its ubiquitination and degradation.
Ubiquitinated by the anaphase promoting complex/cyclosome (APC/C) ubiquitin ligase complex that contains FZR1/CDH1 during G1 phase leading to its degradation by the proteasome. Ubiquitinated by a SCF complex containing BTRC and FBXW11 during S phase leading to its degradation by the proteasome. Deubiquitination by USP17L2/DUB3 leads to its stabilization. - Information by UniProt
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参照データベース
- Entrez Gene: 993 Human
- Omim: 116947 Human
- SwissProt: P30304 Human
- Unigene: 437705 Human
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別名
- Cdc 25a antibody
- CDC25A antibody
- CDC25A2 antibody
see all
画像
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Western blot - Anti-Cdc25A antibody (ab989)All lanes : Anti-Cdc25A antibody (ab989) at 1 µg/ml
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 59 kDa
Exposure time: 3 minutes -
Immunoprecipitation - Anti-Cdc25A antibody (ab989)Cdc25A was immunoprecipitated from HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (0.5 or 1 mg for IP, 20% of IP loaded) with ab989 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab989 at 1 µg/ml.
Lane 1: ab989 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (11)
ab989 は 11 報の論文で使用されています。
- Das S et al. Genome-scale screening of deubiquitinase subfamily identifies USP3 as a stabilizer of Cdc25A regulating cell cycle in cancer. Cell Death Differ N/A:N/A (2020). PubMed: 32415280
- Wang Y et al. P53 suppresses SENP3 phosphorylation to mediate G2 checkpoint. Cell Discov 6:21 (2020). PubMed: 32351703
- Yin W et al. Circular RNA circ_0007142 Facilitates Colorectal Cancer Progression by Modulating CDC25A Expression via miR-122-5p. Onco Targets Ther 13:3689-3701 (2020). PubMed: 32431519
- Huang E et al. CircRNA hsa_circ_0004771 promotes esophageal squamous cell cancer progression via miR-339-5p/CDC25A axis. Epigenomics 12:587-603 (2020). PubMed: 32050790
- Su J et al. Ultrasound-mediated destruction of vascular endothelial growth factor (VEGF) targeted and paclitaxel loaded microbubbles for inhibition of human breast cancer cell MCF-7 proliferation. Mol Cell Probes 46:101415 (2019). PubMed: 31228519