AB989

Anti-Cdc25A 抗体

Name: Anti-Cdc25A 抗体 (ab989) | Abcam
Brand: Abcam Limited
SKU: AB989
Price: 76000 JPY
Availability: InStock
Rating: 3 (1 reviews)

Anti-Cdc25A antibody

(1 Review)

(16 Publications)

Rabbit Polyclonal Cdc25A antibody. Suitable for IP, WB and reacts with Human samples. Cited in 16 publications. Immunogen corresponding to Synthetic Peptide within Human M-phase inducer phosphatase 1 conjugated to Keyhole Limpet Haemocyanin.

別名を表示する

M-phase inducer phosphatase 1, Dual specificity phosphatase Cdc25A, CDC25A

2 Images

Supplier Data

Immunoprecipitation - Anti-Cdc25A antibody (AB989)

Cdc25A was immunoprecipitated from HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (0.5 or 1 mg for IP, 20% of IP loaded) with ab989 at 6 μg/mg lysate. Western blot was performed from the immunoprecipitate using ab989 at 1 μg/ml.

Lane 1 : ab989 IP in HEK-293T whole cell lysate.

Lane 2 : Control IgG IP in HEK-293T whole cell lysate.

All lanes:

Immunoprecipitation - Anti-Cdc25A antibody (ab989)

Predicted band size: 59 kDa

false

Exposure time: 3min

Supplier Data

Western blot - Anti-Cdc25A antibody (AB989)

All lanes:

Western blot - Anti-Cdc25A antibody (ab989) at 1 µg/mL

Lane 1:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 50 µg

Lane 2:

HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Predicted band size: 59 kDa

false

Exposure time: 3min

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Human

アプリケーション

WB, IP

applications

免疫原

Synthetic Peptide within Human M-phase inducer phosphatase 1 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P30304

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Immunogen

バッファー組成

pH: 7 - 8 Preservative: 0.1% Sodium azide Constituents: PBS, 1.815% Tris, 1.764% Sodium citrate

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

+4°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle|Do Not Freeze

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Cdc25A also known as Cell Division Cycle 25A acts as a phosphatase that removes phosphate groups from cyclin-dependent kinases (CDKs). This process mainly activates CDKs which are important for the cell cycle progression. Cdc25A has a molecular mass of approximately 65 kDa. The protein expresses in various cell types but finds higher levels in proliferative tissues. Additionally it localizes primarily in the nucleus where it executes its function during specific cell cycle phases.

Biological function summary

Cdc25A plays a big role in controlling transitions between cell cycle phases particularly the G1 to S phase. It participates in a CDK complex which includes partners like Cyclin E and Cyclin A driving progression through cell cycle checkpoints. During DNA replication Cdc25A's activity ensures that cells duplicate accurately maintaining genome integrity and balance in cell proliferation.

Pathways

Cdc25A functions within the cell cycle regulation and DNA damage repair pathways. It interacts importantly with proteins like Checkpoint Kinase 1 and 2 (Chk1/Chk2) involved in responding to DNA damage. During adverse conditions Chk1/Chk2 phosphorylate Cdc25A leading to its degradation and preventing further cycle progression. This mechanism helps maintain genomic stability by halting the cycle until DNA repair occurs.

Cdc25A holds connections to cancer and neurodegenerative conditions. Its overexpression often links to cancers as uncontrolled activity allows for unscheduled cell proliferation. Similarly dysregulation of Cdc25A relates to proteins like p53 which suppress tumor growth. In neurodegenerative disorders Cdc25A's misregulation can also increase the potential for neuronal cell cycle re-entry contributing to neuronal death. Understanding these relationships is essential for developing therapeutic targets.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression (PubMed : 12676925, PubMed : 14559997, PubMed : 1836978, PubMed : 20360007). Directly dephosphorylates CDK1 and stimulates its kinase activity (PubMed : 20360007). Also dephosphorylates CDK2 in complex with cyclin-E, in vitro (PubMed : 20360007).

See full target information M-phase inducer phosphatase 1

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文献 (16)

Recent publications for all applications. Explore the full list and refine your search

NPJ precision oncology 9:80 PubMed40119070

2025

ARRB2 promotes cervical cancer progression via stabilizing CDC25A mRNA through m6A-IGF2BP1-dependent manner.

Applications

Unspecified application

Species

Unspecified reactive species

Lijie Li,Jie Zeng,Mengying Liu,Hui Cheng,Yuyin He,Sili He,Chen Wang

Molecular medicine reports 27: PubMed37052240

2023

CDC25A inhibition suppresses cell proliferation and induces G/S‑phase cell cycle arrest in nasopharyngeal carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Bin Wang,Qiaohong Gong,Fuhai Chen

CNS neuroscience & therapeutics 29:691-711 PubMed36550594

2022

LncRNA LINC01018/miR-942-5p/KNG1 axis regulates the malignant development of glioma in vitro and in vivo.

Applications

Unspecified application

Species

Unspecified reactive species

Jinfang Xu,Jianli Wang,Mingfei Zhao,Chenguang Li,Shen Hong,Jianmin Zhang

Cell death and differentiation 27:3004-3020 PubMed32415280

2020

Genome-scale screening of deubiquitinase subfamily identifies USP3 as a stabilizer of Cdc25A regulating cell cycle in cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Soumyadip Das,Arun Pandian Chandrasekaran,Bharathi Suresh,Saba Haq,Jae-Hyeok Kang,Su-Jae Lee,Jaewon Kim,Jaesang Kim,Sanghyuk Lee,Hyongbum Henry Kim,Kye-Seong Kim,Suresh Ramakrishna

OncoTargets and therapy 13:3689-3701 PubMed32431519

2020

Circular RNA circ_0007142 Facilitates Colorectal Cancer Progression by Modulating CDC25A Expression via miR-122-5p.

Applications

Unspecified application

Species

Unspecified reactive species

Wenzhe Yin,Jun Xu,Chao Li,Xiankui Dai,Tong Wu,Jifeng Wen

Cell discovery 6:21 PubMed32351703

2020

P53 suppresses SENP3 phosphorylation to mediate G2 checkpoint.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Wang,Jing Tian,Chao Huang,Jiao Ma,Gaolei Hu,Yalan Chen,Tianshi Wang,Rong Cai,Yong Zuo,Hongsheng Tan,Qiuju Fan,Baijun Dong,Wei Xue,Jing Yi,Guoqiang Chen,Jun Tu,Jinke Cheng

Epigenomics 12:587-603 PubMed32050790

2020

CircRNA hsa_circ_0004771 promotes esophageal squamous cell cancer progression via miR-339-5p/CDC25A axis.

Applications

Unspecified application

Species

Unspecified reactive species

Enmin Huang,Junhui Fu,Qiuyan Yu,Peixin Xie,Zhongxian Yang,Huanlin Ji,Li Wang,Ganfeng Luo,Yanting Zhang,Ke Li

Molecular and cellular probes 46:101415 PubMed31228519

2019

Ultrasound-mediated destruction of vascular endothelial growth factor (VEGF) targeted and paclitaxel loaded microbubbles for inhibition of human breast cancer cell MCF-7 proliferation.

Applications

Unspecified application

Species

Unspecified reactive species

Jilian Su,Junmei Wang,Jiamin Luo,Haili Li

Scientific reports 9:3617 PubMed30837643

2019

Effect of CHK1 Inhibition on CPX-351 Cytotoxicity in vitro and ex vivo.

Applications

Unspecified application

Species

Unspecified reactive species

Nicole D Vincelette,Husheng Ding,Amelia M Huehls,Karen S Flatten,Rebecca L Kelly,Mira A Kohorst,Jonathan Webster,Allan D Hess,Keith W Pratz,Larry M Karnitz,Scott H Kaufmann

Journal of cellular physiology 234:3526-3537 PubMed30443946

2018

MicroRNA-99a-5p suppresses breast cancer progression and cell-cycle pathway through downregulating CDC25A.

Applications

Unspecified application

Species

Unspecified reactive species

Hongzhen Qin,Wenfei Liu

View all publications

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Abcam product promise

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For licensing inquiries, please contact partnerships@abcam.com

Anti-Cdc25A 抗体

Anti-Cdc25A antibody

Immunoprecipitation - Anti-Cdc25A antibody (AB989)

Western blot - Anti-Cdc25A antibody (AB989)

Key facts

宿主種

クローン性

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (16)

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Abcam product promise