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AB317037

Anti-Cdc20 抗体 [EPR28091-55] - BSA and Azide free

Anti-Cdc20 antibody [EPR28091-55] - BSA and Azide free

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Rabbit Recombinant Monoclonal CDC20 antibody. Carrier free. Suitable for Flow Cyt (Intra), WB, IP, IHC-P and reacts with Human, Mouse, Rat samples.

別名を表示する

Cell division cycle protein 20 homolog, p55CDC, CDC20

16 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdc20 antibody [EPR28091-55] - BSA and Azide free (AB317037)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdc20 antibody [EPR28091-55] - BSA and Azide free (AB317037)

This data was developed using ab317036, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human ovarian carcinoma tissue labeling Cdc20 with ab317036 at 1/100 (4.96 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on human ovarian carcinoma. The section was incubated with ab317036 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Flow Cytometry (Intracellular) - Anti-Cdc20 antibody [EPR28091-55] - BSA and Azide free (AB317037)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Cdc20 antibody [EPR28091-55] - BSA and Azide free (AB317037)

This data was developed using ab317036, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (human cervical adenocarcinoma epithelial cell) treated with 100ng/mL nocodazole for 16 hours (Red) / Untreated HeLa (Dotted Red) cells labelling Cdc20 with ab317036 at 1/50 dilution (1 ug) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdc20 antibody [EPR28091-55] - BSA and Azide free (AB317037)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdc20 antibody [EPR28091-55] - BSA and Azide free (AB317037)

This data was developed using ab317036, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Cdc20 with ab317036 at 1/100 (4.96 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on human colon (PMID : 26245990). The section was incubated with ab317036 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdc20 antibody [EPR28091-55] - BSA and Azide free (AB317037)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdc20 antibody [EPR28091-55] - BSA and Azide free (AB317037)

This data was developed using ab317036, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling Cdc20 with ab317036 at 1/100 (4.96 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on human tonsil. The section was incubated with ab317036 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdc20 antibody [EPR28091-55] - BSA and Azide free (AB317037)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdc20 antibody [EPR28091-55] - BSA and Azide free (AB317037)

This data was developed using ab317036, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling Cdc20 with ab317036 at 1/100 (4.96 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Low expression : no staining on human cerebrum. The section was incubated with ab317036 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Immunoprecipitation - Anti-Cdc20 antibody [EPR28091-55] - BSA and Azide free (AB317037)
  • IP

Supplier Data

Immunoprecipitation - Anti-Cdc20 antibody [EPR28091-55] - BSA and Azide free (AB317037)

This data was developed using ab317036, the same antibody clone in a different buffer formulation.

Cdc20 was immunoprecipitated from 0.35 mg PC-3 (human prostate adenocarcinoma epithelial cell) whole cell lysate with ab317036 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab317036 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : PC-3 (human prostate adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : ab317036 IP in PC-3 (human prostate adenocarcinoma epithelial cell) whole cell lysate
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab317036 in PC-3 whole cell lysate

The band at ~37KDa may represent a truncated Cdc20 isoform (PMID : 37100900).

All lanes:

Immunoprecipitation - Anti-Cdc20 antibody [EPR28091-55] (<a href='/products/primary-antibodies/cdc20-antibody-epr28091-55-ab317036'>ab317036</a>) at 1/30 dilution

All lanes:

PC-3 (human prostate adenocarcinoma epithelial cell) whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

false

Exposure time: 180s

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdc20 antibody [EPR28091-55] - BSA and Azide free (AB317037)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdc20 antibody [EPR28091-55] - BSA and Azide free (AB317037)

This data was developed using ab317036, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Rat colon tissue labeling Cdc20 with ab317036 at 1/100 (4.96 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on rat colon. The section was incubated with ab317036 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdc20 antibody [EPR28091-55] - BSA and Azide free (AB317037)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdc20 antibody [EPR28091-55] - BSA and Azide free (AB317037)

This data was developed using ab317036, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling Cdc20 with ab317036 at 1/100 (4.96 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Low expression : no staining on mouse cerebrum. The section was incubated with ab317036 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdc20 antibody [EPR28091-55] - BSA and Azide free (AB317037)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdc20 antibody [EPR28091-55] - BSA and Azide free (AB317037)

This data was developed using ab317036, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling Cdc20 with ab317036 at 1/100 (4.96 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Low expression : no staining on rat cerebrum. The section was incubated with ab317036 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdc20 antibody [EPR28091-55] - BSA and Azide free (AB317037)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdc20 antibody [EPR28091-55] - BSA and Azide free (AB317037)

This data was developed using ab317036, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Rat testis tissue labeling Cdc20 with ab317036 at 1/100 (4.96 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on rat testis. The section was incubated with ab317036 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Flow Cytometry (Intracellular) - Anti-Cdc20 antibody [EPR28091-55] - BSA and Azide free (AB317037)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Cdc20 antibody [EPR28091-55] - BSA and Azide free (AB317037)

This data was developed using ab317036, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized NIH/3T3 (mouse embryonic fibroblast) cells labelling Cdc20 with ab317036 at 1/50 dilution (1 ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdc20 antibody [EPR28091-55] - BSA and Azide free (AB317037)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdc20 antibody [EPR28091-55] - BSA and Azide free (AB317037)

This data was developed using ab317036, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labeling Cdc20 with ab317036 at 1/100 (4.96 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on mouse testis. The section was incubated with ab317036 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdc20 antibody [EPR28091-55] - BSA and Azide free (AB317037)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdc20 antibody [EPR28091-55] - BSA and Azide free (AB317037)

This data was developed using ab317036, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling Cdc20 with ab317036 at 1/100 (4.96 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on mouse colon. The section was incubated with ab317036 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Western blot - Anti-Cdc20 antibody [EPR28091-55] - BSA and Azide free (AB317037)
  • WB

Supplier Data

Western blot - Anti-Cdc20 antibody [EPR28091-55] - BSA and Azide free (AB317037)

This data was developed using ab317036, the same antibody clone in a different buffer formulation.

The expression of Cdc20 is upregulated in response to nocodazole treatment. The band at ~37KDa may represent a truncated Cdc20 isoform (PMID : 37100900).

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-Cdc20 antibody [EPR28091-55] (<a href='/products/primary-antibodies/cdc20-antibody-epr28091-55-ab317036'>ab317036</a>) at 1/1000 dilution

Lane 1:

Untreated HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

HeLa treated with 100ng/ml nocodazole for 16 hours whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 51 kDa,36 kDa

false

Exposure time: 37s

Western blot - Anti-Cdc20 antibody [EPR28091-55] - BSA and Azide free (AB317037)
  • WB

Supplier Data

Western blot - Anti-Cdc20 antibody [EPR28091-55] - BSA and Azide free (AB317037)

This data was developed using ab317036, the same antibody clone in a different buffer formulation. Low expression : DU145 (PMID : 27633058), LNCaP (PMID : 27633058).

In lanes 1-4, the lysates were stored at -80°C prior to Western Blotting. In lanes 5-8, the lysates were freshly made and used for Western Blotting immediately to minimize protein degradation.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-Cdc20 antibody [EPR28091-55] (<a href='/products/primary-antibodies/cdc20-antibody-epr28091-55-ab317036'>ab317036</a>) at 1/1000 dilution

Lane 1:

HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 40 µg

Lane 2:

PC-3 (human prostate adenocarcinoma epithelial cell) whole cell lysate at 40 µg

Lane 3:

DU145 (human prostate carcinoma epithelial cell) whole cell lysate at 40 µg

Lane 4:

LNCaP (human prostate carcinoma epithelial cell) whole cell lysate at 40 µg

Lane 5:

HeLa whole fresh cell lysate at 20 µg

Lane 6:

293T (human embryonic kidney epithelial cell) whole fresh cell lysate at 20 µg

Lane 7:

NIH/3T3 (mouse embryonic fibroblast) whole fresh cell lysate at 20 µg

Lane 8:

PC-12 (rat adrenal gland pheochromocytoma) whole fresh cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 51 kDa,36 kDa

false

Exposure time: 180s

Western blot - Anti-Cdc20 antibody [EPR28091-55] - BSA and Azide free (AB317037)
  • WB

Supplier Data

Western blot - Anti-Cdc20 antibody [EPR28091-55] - BSA and Azide free (AB317037)

This data was developed using ab317036, the same antibody clone in a different buffer formulation.

Low expression : kidney (PMID : 15280373), liver (PMID : 15280373), brain (PMID : 15280373).

In lanes 1-6, the lysates were stored at -80°C prior to Western Blotting. In lanes 7-11, the lysates were freshly made and used for Western Blotting immediately to minimize protein degradation.

The identity of the higher MW bands at approximately 74 kDa (in lanes 1-7) and 90 KDa (lane 8) are unknown.

Lanes 4-11 are applied with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjMSD1483ated (ab97051) at 1/100000 and lanes 1-3 are applied with Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

Exposure time : Lanes 1-6, 9-11 : 180 seconds, lanes 7-8 : 70 seconds.

All lanes:

Western blot - Anti-Cdc20 antibody [EPR28091-55] (<a href='/products/primary-antibodies/cdc20-antibody-epr28091-55-ab317036'>ab317036</a>) at 1/1000 dilution

Lane 1:

Human testis tissue lysate at 40 µg

Lane 2:

Human kidney tissue lysate at 40 µg

Lane 3:

Human liver tissue lysate at 40 µg

Lane 4:

Mouse testis tissue lysate at 40 µg

Lane 5:

Mouse thymus tissue lysate at 40 µg

Lane 6:

Mouse kidney tissue lysate at 40 µg

Lane 7:

Mouse testis tissue lysate at 20 µg

Lane 8:

Mouse brain tissue lysate at 20 µg

Lane 9:

Rat testis tissue lysate at 20 µg

Lane 10:

Rat brain tissue lysate at 20 µg

Lane 11:

Rat liver tissue lysate at 20 µg

Secondary

Lanes 1 - 3:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Lanes 4 - 11:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 51 kDa,36 kDa

false

関連する標識済み抗体及び組成の異なる製品 (1)

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR28091-55

アイソタイプ

IgG

キャリアフリー

Yes

交差種

Human, Mouse, Rat

アプリケーション

IP, IHC-P, Flow Cyt (Intra), WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Rat": { "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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製品の詳細

ab317037 is the carrier-free version of ab317036.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.2 - 7.4 Constituents: PBS
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
+4°C
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Cdc20 also known as cell division cycle 20 is an important regulatory protein in the cell cycle. It plays an essential role in the anaphase-promoting complex/cyclosome (APC/C) a large ubiquitin ligase complex important for the progression of the cell cycle. Cdc20 is expressed in various tissues acting as a cell cycle regulator during mitosis. It has a molecular weight of approximately 55 kDa. The Cdc20 protein ensures the timely degradation of key mitotic inhibitors facilitating proper chromosome segregation and mitotic exit by tagging these inhibitors for proteasomal degradation.
Biological function summary

Cdc20 acts as an activator of the APC/C complex regulating the transition from metaphase to anaphase during cell division. This transition depends on the degradation of securin and cyclins ensuring correct chromosome separation and cell cycle progression. By forming a complex with APC/C Cdc20 functions to target proteins for destruction modulating cell division dynamically. Its activity ensures that chromosomes are correctly aligned and segregated preventing errors during cell division that may lead to genomic instability.

Pathways

Cdc20 integrates itself into the spindle assembly checkpoint (SAC) and the mitotic exit network (MEN). These pathways monitor chromosome alignment and ensure that cells do not proceed to anaphase until all chromosomes are properly attached to the spindle apparatus. SAC utilizes Cdc20 to regulate its inhibitory role until chromosome alignment is confirmed after which Cdc20 activates APC/C to drive anaphase onset. Moreover proteins such as BubR1 interact with Cdc20 within the SAC to inhibit premature activation of APC/C while conditions are not optimal.

Cdc20 has significant connections with cancer and chromosomal instability syndromes. Overexpression or malfunction of Cdc20 often links with various cancers such as colorectal cancer where it drives an uncontrolled cell division rate and contributes to tumorigenesis. Additionally the improper regulation of Cdc20 can impact proteins like Mad2 disrupting the spindle checkpoint and leading to aneuploidy. This disruption often ties to chromosomal instability observed in cancerous cells highlighting its role as a potential target for therapeutic intervention.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Substrate-specific adapter of the anaphase promoting complex/cyclosome (APC/C) complex that confers substrate specificity by binding to substrates and targeting them to the APC/C complex for ubiquitination and degradation (PubMed : 9734353, PubMed : 27030811, PubMed : 29343641). Recognizes and binds the destruction box (D box) on protein substrates (PubMed : 29343641). Involved in the metaphase/anaphase transition of cell cycle (PubMed : 32666501). Is regulated by MAD2L1 : in metaphase the MAD2L1-CDC20-APC/C ternary complex is inactive and in anaphase the CDC20-APC/C binary complex is active in degrading substrates (PubMed : 9811605, PubMed : 9637688). The CDC20-APC/C complex positively regulates the formation of synaptic vesicle clustering at active zone to the presynaptic membrane in postmitotic neurons (By similarity). CDC20-APC/C-induced degradation of NEUROD2 induces presynaptic differentiation (By similarity). The CDC20-APC/C complex promotes proper dilation formation and radial migration by degrading CCDC41 (By similarity).
See full target information Cell division cycle protein 20 homolog
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組合せ製品

Primary Antibodies

AB9485

Anti-GAPDH antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (AB9485)

  • 5
  • 88
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB15580

Anti-Ki67 antibody

BOND RX™ Validated|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody (AB15580)

  • 5
  • 230
Primary Antibodies

AB195268

Anti-Bub1 antibody [EPR18947]

RabMAb|Recombinant|KO Validated|20ul selling size

Immunocytochemistry/ Immunofluorescence - Anti-Bub1 antibody [EPR18947] (AB195268)

  • 0
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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。
保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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