Anti-Cdc20 抗体 (ab64877)
Key features and details
- Rabbit polyclonal to Cdc20
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Cdc20 antibody
Cdc20 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Cdc20 -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide (Human) from an internal sequence of Cdc20.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab64877の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).
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| 特記事項 |
|---|
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WB
1/500 - 1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa). |
ターゲット情報
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機能
Required for full ubiquitin ligase activity of the anaphase promoting complex/cyclosome (APC/C) and may confer substrate specificity upon the complex. Is regulated by MAD2L1. In metaphase the MAD2L1-CDC20-APC/C ternary complex is inactive and in anaphase the CDC20-APC/C binary complex is active in degrading substrates. -
パスウェイ
Protein modification; protein ubiquitination. -
配列類似性
Belongs to the WD repeat CDC20/Fizzy family.
Contains 7 WD repeats. -
発生段階
Synthesis is initiated at G1/S, protein level peaks in M phase and protein is abruptly degraded at M/G1 transition. -
翻訳後修飾
Phosphorylated during mitosis, probably by maturation promoting factor (MPF). Phosphorylated by BUB1 at Ser-41; Ser-72; Ser-92; Ser-153; Thr-157 and Ser-161.
Ubiquitinated and degraded by the proteasome during spindle assembly checkpoint. Deubiquitinated by USP44, leading to stabilize the MAD2L1-CDC20-APC/C ternary complex, thereby preventing premature activation of the APC/C. - Information by UniProt
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参照データベース
- Entrez Gene: 991 Human
- Omim: 603618 Human
- SwissProt: Q12834 Human
- Unigene: 524947 Human
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別名
- bA276H19.3 antibody
- Cdc 20 antibody
- CDC20 antibody
see all
画像
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Western blot - Anti-Cdc20 antibody (ab64877)All lanes : Anti-Cdc20 antibody (ab64877) at 1/500 dilution
Lane 1 : extract from HeLa cells, (5-30µg total protein).
Lane 2 : extract from Jurkat cells, (5-30µg total protein).
Lane 3 : extract from Jurkat cells, (5-30µg total protein) and 5-10µg of the immunising peptide.
Predicted band size: 55 kDa
Observed band size: 55 kDa
Additional bands at: 26 kDa, 34 kDa. We are unsure as to the identity of these extra bands.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (5)
ab64877 は 5 報の論文で使用されています。
- Park YY et al. ATP depletion during mitotic arrest induces mitotic slippage and APC/CCdh1-dependent cyclin B1 degradation. Exp Mol Med 50:46 (2018). PubMed: 29700288
- Tan CL et al. Stranglehold on the spindle assembly checkpoint: the human papillomavirus E2 protein provokes BUBR1-dependent aneuploidy. Cell Cycle 14:1459-70 (2015). WB ; Human . PubMed: 25789401
- Adhikari D et al. Mastl is required for timely activation of APC/C in meiosis I and Cdk1 reactivation in meiosis II. J Cell Biol 206:843-53 (2014). Mouse . PubMed: 25246615
- Uzunova K et al. APC15 mediates CDC20 autoubiquitylation by APC/C(MCC) and disassembly of the mitotic checkpoint complex. Nat Struct Mol Biol 19:1116-23 (2012). WB ; Human . PubMed: 23007861
- Davoli T et al. Persistent telomere damage induces bypass of mitosis and tetraploidy. Cell 141:81-93 (2010). PubMed: 20371347