Anti-CD99 抗体 (ab27271)
Key features and details
- Rabbit polyclonal to CD99
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-CD99 antibody
CD99 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to CD99 -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, WBmore details -
種交差性
交差種: Human -
ポジティブ・コントロール
- Human pancreas or Ewing's sarcoma for Immunohistochemistry.
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特記事項
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.60
Preservative: 0.08% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
This antibody is affinity purified. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab27271の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P | ||
WB |
特記事項 |
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Staining pattern: Cell membrane
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 19 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
ターゲット情報
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機能
Involved in T-cell adhesion processes. It is involved in spontaneous rosette formation with erythrocytes. -
配列類似性
Belongs to the CD99 family. -
翻訳後修飾
Extensively O-glycosylated. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 4267 Human
- Omim: 313470 Human
- SwissProt: P14209 Human
- Unigene: 653349 Human
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別名
- 12E7 antibody
- Antigen identified by monoclonal 12E7, Y homolog antibody
- Antigen identified by monoclonal antibodies 12E7, F21 and O13 antibody
see all
画像
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Ab27271, prediluted, staining CD99 in paraffin embedded, formalin fixed human pancreas tissue by Immunohistochemistry.
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All lanes : Anti-CD99 antibody (ab27271) at 1 µg/ml
Lane 1 : Human thymus tissue lysate - total protein (ab30146)
Lane 2 : Human testis tissue lysate - total protein (ab30257)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size: 19 kDa
Observed band size: 32 kDa why is the actual band size different from the predicted?
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (5)
ab27271 は 5 報の論文で使用されています。
- Scopim-Ribeiro R et al. NSG Mice Facilitate ex vivo Characterization of Ewing Sarcoma Lung Metastasis Using the PuMA Model. Front Oncol 11:645757 (2021). PubMed: 33828989
- Huijbers EJM et al. Targeting Tumor Vascular CD99 Inhibits Tumor Growth. Front Immunol 10:651 (2019). PubMed: 31001265
- Cooper A et al. Ewing tumors that do not overexpress BMI-1 are a distinct molecular subclass with variant biology: a report from the Children's Oncology Group. Clin Cancer Res 17:56-66 (2011). IHC-P ; Human . PubMed: 21047978
- Makhoul R et al. Paranuclear dot-like immunostaining for CD99: presence in colonic adenomas and adenocarcinomas. Am J Surg Pathol 35:1749-50 (2011). PubMed: 21997696
- Luo W et al. GSTM4 is a microsatellite-containing EWS/FLI target involved in Ewing's sarcoma oncogenesis and therapeutic resistance. Oncogene 28:4126-32 (2009). IHC ; Human . PubMed: 19718047