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AB236271

Anti-CD86 抗体 [C86/2160R] - BSA and Azide free

Anti-CD86 antibody [C86/2160R] - BSA and Azide free

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(1 Review)

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(1 Publication)

Rabbit Recombinant Monoclonal CD86 antibody. Carrier free. Suitable for IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human CD86.

別名を表示する

CD86, CD28LG2, T-lymphocyte activation antigen CD86, Activation B7-2 antigen, B70, BU63, CTLA-4 counter-receptor B7.2, FUN-1

1 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD86 antibody [C86/2160R] - BSA and Azide free (AB236271)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD86 antibody [C86/2160R] - BSA and Azide free (AB236271)

Formalin-fixed, paraffin-embedded human colon tissue stained for CD86 with ab234401 at 4 μg/ml in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab234401).

関連する標識済み抗体及び組成の異なる製品 (1)

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

C86/2160R

アイソタイプ

IgG

キャリアフリー

Yes

交差種

Human

アプリケーション

IHC-P

applications

免疫原

Recombinant Full Length Protein corresponding to Human CD86.

P42081

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代替製品

Primary Antibodies

AB213045

Anti-CD86 antibody [C86/1146] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD86 antibody [C86/1146] - BSA and Azide free (AB213045)

  • 0
  • 0
Primary Antibodies

AB256270

Alexa Fluor® 488 Anti-CD86 antibody [OX48]

Flow Cytometry - Alexa Fluor® 488 Anti-CD86 antibody [OX48] (AB256270)

  • 0
  • 0
Primary Antibodies

AB256271

Alexa Fluor® 647 Anti-CD86 antibody [OX48]

Flow Cytometry - Alexa Fluor® 647 Anti-CD86 antibody [OX48] (AB256271)

  • 0
  • 0
Primary Antibodies

AB242020

Anti-CD86 antibody [EPR21962] - BSA and Azide free

RabMAb|Recombinant|KO Validated

Flow Cytometry - Anti-CD86 antibody [EPR21962] - BSA and Azide free (AB242020)

  • 0
  • 0
Primary Antibodies

AB239075

Anti-CD86 antibody [EPR21962]

20ul selling size|RabMAb|Recombinant|KO Validated

Flow Cytometry - Anti-CD86 antibody [EPR21962] (AB239075)

  • 0
  • 0

組合せ製品

Assay Kits

AB45921

Human CD86 ELISA Kit

ELISA - Human CD86 ELISA Kit (AB45921)

  • 0
  • 0
Primary Antibodies

AB64693

Anti-CD206 antibody

BOND RX™ Validated

Western blot - Anti-CD206 antibody (AB64693)

  • 5
  • 42
Primary Antibodies

AB182422

Anti-CD163 antibody [EPR19518]

BOND RX™ Validated|20ul selling size|RabMAb|Advanced Validation|Recombinant

Flow Cytometry - Anti-CD163 antibody [EPR19518] (AB182422)

  • 5
  • 17
Primary Antibodies

AB124964

Anti-alpha smooth muscle Actin antibody [EPR5368]

BOND RX™ Validated|RabMAb|Recombinant|KO Validated|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [EPR5368] (AB124964)

  • 5
  • 19
Primary Antibodies

AB955

Anti-CD68 antibody [KP1]

Recombinant|Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [KP1] (AB955)

  • 4
  • 48
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "2-4 µg/mL", "IHCP-species-notes": "<p>Incubate with primary antibody for 30 minutes at room temperature.</p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." } } }
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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A/G
精製に関する特記事項
Purified from bioreactor concentrate by Protein A/G.
バッファー組成
pH: 7.2 - 7.4 Constituents: PBS
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

CD86 also known as B7-2 is a protein involved in the regulation of the immune response. It has an approximate mass of 70 kDa and is expressed on antigen-presenting cells like dendritic cells monocytes and macrophages. Notably CD86 is present on macrophages including those in tissues such as skin and lymphoid organs. Expressed on these cells CD86 serves as a vital mediator in the co-stimulatory signals necessary for T cell activation and survival.
Biological function summary

CD86 plays a significant role in the immune system by providing secondary signals for T cell activation and differentiation. It is a part of the B7 protein family and forms a complex with CD28 and CTLA-4 on T cells. When CD86 binds to CD28 it sends positive co-stimulatory signals which promote T cell proliferation and cytokine production. On the other hand interaction with CTLA-4 transmits an inhibitory signal which reduces immune response. This dual interaction helps to balance immune activation and tolerance.

Pathways

CD86 takes part in important immune-related signaling pathways particularly the T cell receptor signaling pathway and the PI3K-Akt signaling pathway. Both pathways are fundamental for initiating immune responses. CD86's interaction with CD28 activates downstream signaling cascades including PI3K-Akt which is important for cell survival and growth. Additionally CD86 collaborates with other proteins such as CD80 another co-stimulatory molecule to amplify T cell activation within these pathways.

CD86 is associated with autoimmune diseases and transplant rejection. In autoimmune diseases like rheumatoid arthritis the overexpression or dysregulation of CD86 can lead to excessive T cell activation causing immune system attacks on the body's own tissues. Similarly in transplant rejection CD86 may contribute by enhancing immune response against transplanted organs. The engagement between CD86 and CD28 is a critical factor in these conditions and therapies targeting this interaction are under exploration to mitigate the immune response.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Costimulatory molecule that belongs to the immunoglobulin superfamily that plays an important role in T-lymphocyte activation (PubMed : 12196291, PubMed : 7694363). Acts as the primary auxiliary signal augmenting the MHC/TCR signal in naive T-cells by acting as a ligand for the CD28 receptor which is constitutively expressed on the cell surface of T-cells (PubMed : 12196291, PubMed : 7694363). May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation (PubMed : 7527824). Also involved in the regulation of B cells function, plays a role in regulating the level of IgG(1) produced. Upon CD40 engagement, activates NF-kappa-B signaling pathway via phospholipase C and protein kinase C activation (By similarity). Also acts as an inhibitor of T-cell activation by acting as a ligand for CTLA4, a decoy receptor, thereby blocking CD28-mediated T-cell priming (PubMed : 11279501).. Isoform 2. Interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation.. (Microbial infection) Acts as a receptor for adenovirus subgroup B.
See full target information CD86
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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 12:e2415205 PubMed40151877

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Applications

Unspecified application

Species

Unspecified reactive species

Shiyu Hu,Feng Zhang,Jingpu Wang,Jian Zhang,Chenguang Li,Yang Lyu,Yiwen Wang,Rong Huang,Yang Gao,Hongbo Yang,Juying Qian,Wenwen Tang,Jiatian Cao,Junbo Ge
View all publications
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