Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
遺伝子ノックアウト細胞株 検証済

Anti-CD81 抗体 [1D6] - Low endotoxin, Azide free (ab35026)

Reviews (1)Q&A (1)References (6)
Flow Cytometry - Anti-CD81 antibody [1D6] - Low endotoxin, Azide free (ab35026)

    Key features and details

    • Mouse monoclonal [1D6] to CD81 - Low endotoxin, Azide free
    • Suitable for: Flow Cyt
    • Knockout validated
    • Reacts with: Human
    • Isotype: IgG1

    Conjugates logo Related conjugates and formulations

    FITC

    製品の概要

    • 製品名

      Anti-CD81 antibody [1D6] - Low endotoxin, Azide free
      CD81 一次抗体 製品一覧

    • 製品の詳細

      Mouse monoclonal [1D6] to CD81 - Low endotoxin, Azide free

    • 由来種

      Mouse

    • 特異性

      ab35026 recognises CD81, a 26kD cell surface antigen and a member of the tetraspanin family.

    • アプリケーション

      適用あり: Flow Cytmore details
      適用なし: WB

    • 種交差性

      交差種: Human

    • 免疫原

      Tissue, cells or virus corresponding to Human CD81. Aggregated OCI-LY8 cells.

    • ポジティブ･コントロール

      • Flow Cyt: HAP1-WT cells.

    • 特記事項

      ab35026 is a potent CD81 reagent, induces homotypic adhesion and has powerful anti proliferative effects.

      Endotoxin Level: <0.01 Eu/ug (low endotoxin grade).

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    製品の特性

    • 製品の状態

      Liquid

    • 保存方法

      Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    • バッファー

      pH: 7.40
      Constituent: PBS

    • キャリア・フリー

      はい
    • Concentration information loading...

    • 精製度

      Protein G purified

    • 特記事項（精製）

      This antibody was purified from tissue culture supernatant.

    • 一次抗体 備考

      Ab35026 is a potent CD81 reagent, induces homotypic adhesion and has powerful anti proliferative effects. Endotoxin Level: <0.01 Eu/ug (low endotoxin grade).

    • ポリ/モノ

      モノクローナル

    • クローン名

      1D6

    • ミエローマ

      P3x63-Ag8.653

    • アイソタイプ

      IgG1

    • 研究分野

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab35026の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    Flow Cyt
    1/5 - 1/10.

    Use 10µl of the suggested working dilution to label 106 cells in 100µl.
    ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
    特記事項
    Flow Cyt
    1/5 - 1/10.

    Use 10µl of the suggested working dilution to label 106 cells in 100µl.
    ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
    追加情報
    Is unsuitable for WB.

    ターゲット情報

    • 機能

      May play an important role in the regulation of lymphoma cell growth. Interacts with a 16-kDa Leu-13 protein to form a complex possibly involved in signal transduction. May acts a the viral receptor for HCV.

    • 組織特異性

      Hematolymphoid, neuroectodermal and mesenchymal tumor cell lines.

    • 関連疾患

      Defects in CD81 are the cause of immunodeficiency common variable type 6 (CVID6) [MIM:613496]; also called antibody deficiency due to CD81 defect. CVID6 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B cells is usually in the normal range, but can be low.

    • 配列類似性

      Belongs to the tetraspanin (TM4SF) family.

    • 翻訳後修飾

      Not glycosylated.

    • 細胞内局在

      Membrane.
    • Information by UniProt

    • 参照データベース

      • 別名

        • 26 kDa cell surface protein TAPA 1 antibody
        • 26 kDa cell surface protein TAPA-1 antibody
        • 26 kDa cell surface protein TAPA1 antibody
        • CD 81 antibody
        • CD81 antibody
        • CD81 antigen (target of antiproliferative antibody 1) antibody
        • CD81 antigen antibody
        • CD81 molecule antibody
        • CD81_HUMAN antibody
        • CVID6 antibody
        • S5.7 antibody
        • TAPA 1 antibody
        • TAPA1 antibody
        • Target of the antiproliferative antibody 1 antibody
        • Tetraspanin 28 antibody
        • Tetraspanin-28 antibody
        • Tetraspanin28 antibody
        • Tspan 28 antibody
        • Tspan-28 antibody
        • Tspan28 antibody
        see all

      画像

      • Flow Cytometry - Anti-CD81 antibody [1D6] - Low endotoxin, Azide free (ab35026)
        Flow Cytometry - Anti-CD81 antibody [1D6] - Low endotoxin, Azide free (ab35026)

        Overlay histogram showing HAP1 wildtype (green line) and HAP1-CD81 knockout cells (red line) stained with ab35026. The cells were fixed with 4% formaldehyde (10 min)  and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab35026, 0.1µg/ml) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) presorbed (ab150117) at 1/2000 dilution for 30 min at 22°C.

        A mouse IgG1 isotype control antibody  (ab170190) was used at the same concentration and conditions as the primary antibody (HAP1 wildtype - black line, HAP1-CD81  knockout - grey line). Unlabelled sample was also used as a control (this line is not shown for the purpose of simplicity).

        Acquisition of >5,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter.

        This antibody can also be used in HAP1 cells fixed with 80% methanol (5 min), permeabilized with 0.1% PBS-Triton X-100 for 15 min under the same conditions.

      参考文献 (6)

      ab35026 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab35026 は 6 報の論文で使用されています。

      • Scognamiglio I  et al. Exosomal microRNAs synergistically trigger stromal fibroblasts in breast cancer. Mol Ther Nucleic Acids 28:17-31 (2022). PubMed: 35317202
      • Baruah P  et al. TLR9 Mediated Tumor-Stroma Interactions in Human Papilloma Virus (HPV)-Positive Head and Neck Squamous Cell Carcinoma Up-Regulate PD-L1 and PD-L2. Front Immunol 10:1644 (2019). PubMed: 31379843
      • Wu B  et al. Exosomes isolated from CAPS1-overexpressing colorectal cancer cells promote cell migration. Oncol Rep 42:2528-2536 (2019). PubMed: 31638236
      • Yokoi A  et al. Mechanisms of nuclear content loading to exosomes. Sci Adv 5:eaax8849 (2019). PubMed: 31799396
      • Ngalame NNO  et al. Arsenic Alters Exosome Quantity and Cargo to Mediate Stem Cell Recruitment Into a Cancer Stem Cell-Like Phenotype. Toxicol Sci 165:40-49 (2018). PubMed: 30169766
      • Drummond HA  et al. Renal inflammation and elevated blood pressure in a mouse model of reduced {beta}-ENaC. Am J Physiol Renal Physiol 301:F443-9 (2011). WB ; Mouse . PubMed: 21543417

      レビューと Q&A

      Show All レビュー Q&A
      レビューを送る 質問を送る

      1-2 of 2 Abreviews or Q&A

      Immunocytochemistry/ Immunofluorescence abreview for Anti-TAPA1 antibody [1D6]

       Good
      Abreviews
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Human Cell (SH-SY5Y were transfected with cDNA encoding CD81)
      Permeabilization
      Yes - 0,2% Triton PBS
      Specification
      SH-SY5Y were transfected with cDNA encoding CD81
      Blocking step
      BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
      Fixative
      Paraformaldehyde
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      投稿 Jul 29 2015

      Question

      Phone call requesting information on if the Anti-TAPA1 antibody [1D6] - Azide free (ab35026) would be suitable for staining cultured cells.

      Read More
      Answer

      Thank you for contacting us today in regards to the Anti-TAPA1 antibody [1D6] - Azide free (ab35026).

      As discussed over the phone, I have checked into whether the antibody has been used in ICC. I can confirm that the antibody has been used in the staining of HUVEC cells and found to work well in this application. Unfortunately I do not have the exact protocol used, or an image of the results but the antibody should be compatible with this application.

      We would be very interested to hear how you get on with your experiments. If you would like some guidelines as to how to set up your experiments we have quite a good protocol guide which can be accessed from the following link:

      https://docs.abcam.com/pdf/misc/abcam-protocols-book-2010.pdf

      I would also like to draw attention to our Abreview system. If you were to submit the results you have observed with the antibody (by filling in an Abreview, should only take 5-10 minutes), you would rewarded with Abpoints which can be exchanged for either Amazon vouchers or money off your next order with Abcam. You would be rewarded separately for the Western blotting and ICC experiments. For more information on this offer, please follow the link below:

      www.abcam.com/abreviews

      I hope this has been of help. If I can be of any further assistance, please do not hesitate to let me know.

      Read More

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com