Anti-CD80 antibody [EPR28978-182] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD80 antibody [EPR28978-182] - BSA and Azide free (AB325966)

This data was developed using ab322922, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded (A) HEK-293T (human embryonic kidney epithelial cell) transfected with a mouse CD80 expression vector containing a Myc-His tag. (B) HEK-293T transfected with empty vector containing a Myc-His tag. tissue labeling CD80 with ab322922 at 1/2000 (0.257 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on HEK-293T transfected with a Myc-His-tagged mouse CD80 construct (image A). No staining on HEK-293T transfected with empty plasmid (image B). The section was incubated with ab322922 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0 Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD80 antibody [EPR28978-182] - BSA and Azide free (AB325966)

This data was developed using ab322922, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse cardiac muscle tissue labeling CD80 with ab322922 at 1/2000 (0.257 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : no staining on mouse cardiac muscle. The section was incubated with ab322922 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Slides were incubated with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0 Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD80 antibody [EPR28978-182] - BSA and Azide free (AB325966)

This data was developed using ab322922, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse pancreas tissue labeling CD80 with ab322922 at 1/2000 (0.257 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : no staining on mouse pancreas. The section was incubated with ab322922 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Slides were incubated with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0 Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD80 antibody [EPR28978-182] - BSA and Azide free (AB325966)

This data was developed using ab322922, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse pancreatic tumor tissue labeling CD80 with ab322922 at 1/2000 (0.257 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on mouse pancreatic tumor. The section was incubated with ab322922 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Slides were incubated with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0 Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD80 antibody [EPR28978-182] - BSA and Azide free (AB325966)

This data was developed using ab322922, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded (A) Untreated mouse lung. (B) Mouse lung treated with Lipopolysaccharide (1 ug/mL) and Brefeldin A (1 ug/mL) for 16 hours. tissue labeling CD80 with ab322922 at 1/2000 (0.257 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on (A) scattered cells of untreated mouse lung and positive staining on (B) mouse lung treated with Lipopolysaccharide (1 ug/mL) and Brefeldin A (1 ug/mL) for 16 hours. The section was incubated with ab322922 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Slides were incubated with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0 Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD80 antibody [EPR28978-182] - BSA and Azide free (AB325966)

This data was developed using ab322922, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded (A) Untreated mouse spleen. (B) Mouse spleen treated with Lipopolysaccharide (1 ug/mL) for 16 hours and Brefeldin A (1 ug/mL) for overnight. tissue labeling CD80 with ab322922 at 1/2000 (0.257 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Weak staining on (A) scattered cells of untreated mouse spleen and (B) positive staining on mouse spleen treated with Lipopolysaccharide (1 ug/mL) for 16 hours and Brefeldin A (1 ug/mL) for overnight. The section was incubated with ab322922 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Slides were incubated with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0 Epitope Retrieval Solution2) for 20 mins

• mIHC

Supplier Data

Multiplex immunohistochemistry - Anti-CD80 antibody [EPR28978-182] - BSA and Azide free (AB325966)

This data was developed using ab322922, the same antibody clone in a different buffer formulation.

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded mouse spleen treated with lipopolysaccharide (1 ug/ml) for 16 hours and brefeldin a (1 ug/ml) for overnight tissue staining CD80 with ab322922 at a 1 : 2000 (0.257 ug/ml) dilution ab245235 anti-CD19 used at 1 : 1000 (0.444 ug/ml) dilution.

Panel A : merged staining of anti-CD80 (green; Opal™520) and anti-CD19 (magenta; Opal™570) on mouse spleen treated with Lipopolysaccharide.
Panel B : anti-CD80 staining immune cells in mouse spleen treated with Lipopolysaccharide.
Panel C : ant-CD19 staining B lymphocytes in mouse spleen treated with Lipopolysaccharide.
Panel D : Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab322922 and ab245235 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Nuclear counter stain with DAPI.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0 epitope retrieval solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD80 antibody [EPR28978-182] - BSA and Azide free (AB325966)

This data was developed using ab322922, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse thymus tissue labeling CD80 with ab322922 at 1/2000 (0.257 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on mouse thymus. The section was incubated with ab322922 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Slides were incubated with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0 Epitope Retrieval Solution2) for 20 mins