Anti-CD8 beta 抗体 [EPR26538-16] (ab300067)
![Immunocytochemistry/ Immunofluorescence - Anti-CD8 beta antibody [EPR26538-16] (AB300067)](https://www.abcam.co.jp/ps/products/300/ab300067/Images/AB300067-2-anti-cd8-beta-antibody-epr2653816-immunocytochemistry-human-pbmc.jpg "Immunocytochemistry/ Immunofluorescence - Anti-CD8 beta antibody [EPR26538-16] (AB300067)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR26538-16] to CD8 beta
- Suitable for: Flow Cyt, ICC/IF
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-CD8 beta antibody [EPR26538-16]
CD8 beta 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR26538-16] to CD8 beta -
由来種
Rabbit -
アプリケーション
適用あり: Flow Cyt, ICC/IFmore details
適用なし: IHC-Fr,IHC-P,IP or WB -
種交差性
交差種: Human
非交差種: Mouse, Rat -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- ICC/IF: human PBMC cells. Flow Cyt: Human peripheral blood mononuclear cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR26538-16 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab300067の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Flow Cyt |
1/500.
|
|
| ICC/IF |
1/500.
|
| 特記事項 |
|---|
|
Flow Cyt
1/500. |
|
ICC/IF
1/500. |
ターゲット情報
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機能
Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. -
組織特異性
Isoform 1, isoform 3, isoform 5, isoform 6, isoform 7 and isoform 8 are expressed in both thymus and peripheral CD8+ T-cells. Expression of isoform 1 is higher in thymus CD8+ T-cells than in peripheral CD8+ T-cells. Expression of isoform 6 is higher in peripheral CD8+ T-cells than in thymus CD8+ T-cells. -
配列類似性
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
翻訳後修飾
Phosphorylated as a consequence of T-cell activation. -
細胞内局在
Secreted and Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 926 Human
- Omim: 186730 Human
- SwissProt: P10966 Human
- Unigene: 405667 Human
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別名
- CD8b antigen antibody
- CD_antigen=CD8b antibody
- CD8 antigen 37 kDa chain antibody
see all
画像
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Immunocytochemistry/ Immunofluorescence - Anti-CD8 beta antibody [EPR26538-16] (AB300067)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeilized human PBMC (human Peripheral blood mononuclear cell) cells lebelling CD8 beta with ab300067 at 1/500 (1.04 µg/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 µg/ml) dilution (Green). Confocal image showing cytoplasmic and membranous staining in subsets of human PBMCs. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5 µg/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 µg/ml) dilution.
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![Flow Cytometry - Anti-CD8 beta antibody [EPR26538-16] (AB300067)](https://www.abcam.co.jp/ps/products/300/ab300067/Images/AB300067-3-anti-cd8-beta-antibody-epr2653816-flow-cytometry-human-peripheral-blood-mononuclear-cell.jpg)
Flow Cytometry - Anti-CD8 beta antibody [EPR26538-16] (AB300067)Flow cytometric analysis of Human peripheral blood mononuclear cell (PBMC) cells labelling CD8 beta with ab300067 at 1/500 dilution (0.1 μg) / Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control. Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody. Gated on viable cells. Cells were stained with rabbit IgG or ab300067, then stained with anti-CD3 conjugated to Alexa Fluor® 647.
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![Anti-CD8 beta antibody [EPR26538-16] (AB300067)](https://www.abcam.co.jp/ps/products/300/ab300067/Images/AB300067-485286-Benefits-of-Recombinant-antibodies.jpg)
Anti-CD8 beta antibody [EPR26538-16] (AB300067)
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (0)
ab300067 は論文での使用が確認できていません。