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AB4055

Anti-CD8 alpha 抗体

Anti-CD8 alpha antibody

4

(14 Reviews)

|

(184 Publications)

Anti-CD8 alpha antibody (ab4055) is a rabbit polyclonal antibody detecting CD8 alpha in IHC-P. Suitable for Human.

- Over 180 publications
- Trusted since 2003

別名を表示する

CD8a, MAL, CD8A, T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 alpha antibody (AB4055)
  • IHC-P

AbReview60142****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 alpha antibody (AB4055)

ab4055 staining CD8 alpha in human spleen tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 2% BSA for 10 minutes at 21°C; antigen retrieval was by heat mediation in citric acid. Samples were incubated with the primary antibody (1/100 in TBS/BSA/azide) for 16 hours at 21°C. A biotin-conjugated goat anti-rabbit IgG polyclonal (1/300) was used as the secondary antibody.

This image is courtesy of an Abreview submitted by Carl Hobbs.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 alpha antibody (AB4055)
  • IHC-P

AbReview34984****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 alpha antibody (AB4055)

ab4055 staining CD8 alpha in Human lymph node tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked for 5 minutes at 25°C; antigen retrieval was by heat mediation. Samples were incubated with primary antibody (1/200) for 1 hour at 25°C. A Biotin-conjugated Donkey anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody.

This image is courtesy of an Abreview submitted by R Berahovich

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 alpha antibody (AB4055)
  • IHC-P

AbReview6718****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 alpha antibody (AB4055)

ab4055 at 1/500 dilution staining CD8 alpha from human PBMC (Peripheral blood mononuclear cells) tissue sections by IHC-P. The tissue was paraformaldehyde fixed and a heat mediated antigen retrieval step was performed prior to incubation with the antibody for 20 minutes. A biotinylated goat anti-rabbit antibody was used as the secondary.

This image is courtesy of an Abreview submitted by Miss Jill Montgomery

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 alpha antibody (AB4055)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 alpha antibody (AB4055)

ab4055 at 1/200 staining CD8 alpha in human tonsil tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded sections).

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

No

交差種

Human

アプリケーション

IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

特異性

From Mar 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help. You may also be interested in our alternative recombinant antibody, ab178089.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200", "IHCP-species-notes": "<p></p>" }, "Rhesus monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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下記製品もご検討ください

AB178089

Anti-CD8 alpha antibody [SP239]

3
1 Reviews
製品ページを見る
こちらの製品も推奨する理由

私たちは、より高品質な製品をお届けするために、製品構成を常に改善しています。今回ご紹介する代替製品は、性能の向上、使いやすさや利便性の向上、またはより早くお届けできる点などを踏まえて選定しています。

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製品の詳細

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Immunogen
バッファー組成
pH: 7.4 Preservative: 0.1% Sodium azide Constituents: PBS, 1% BSA
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

CD8 alpha also known as CD8A or CD8 protein is a glycoprotein subunit expressed on the surface of cytotoxic T lymphocytes. It has a mass of approximately 32 kDa. Found on the surface cell membrane CD8 alpha functions primarily in the immune response specifically in the recognition of antigens bound to major histocompatibility complex (MHC) class I molecules. Often scientists use CD8 antibodies for detection and CD8 IHC or immunohistochemistry for localization studies.
Biological function summary

The CD8 alpha protein plays a critical role in T-cell mediated immune responses. It forms a heterodimer with the CD8 beta chain creating the CD8 alpha-beta complex that strengthens T-cell interaction with antigen-presenting cells. CD8 alpha also helps in signaling processes that activate T cells equipping them to destroy infected or malignant cells. Researchers often study CD8 alpha peptides to understand its interactions better.

Pathways

CD8 alpha is integral to the T-cell receptor signaling pathway and the cytotoxic T lymphocyte (CTL) pathway. The T-cell receptor complex which includes the CD8 molecule transmits signals that are important for T-cell activation and function. CD8 interacts with key proteins such as the T-cell receptor (TCR) and MHC class I molecules facilitating targeted responses against pathogens. These pathways highlight CD8 alpha’s role in adaptive immunity.

CD8 alpha is most prominently associated with viral infections and cancer. Conditions like HIV and some forms of leukemia show altered CD8 function highlighting the protein's role in immune surveillance. In HIV infection for instance CD8 T cells reduce in number impairing the immune response. CD8 alpha’s connection to the immune system places it alongside other immune proteins such as CD4 and MHC molecules in the context of immune dysfunction.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

The protein expressed by the gene CD8A is an integral membrane glycoprotein crucial for immune responses, operating mainly in T-cells as a coreceptor for MHC class I molecule : peptide complexes. These peptides originate from cytosolic proteins, unlike class II peptides, which are from extracellular proteins. CD8A interacts with the T-cell receptor (TCR) and MHC class I proteins on antigen-presenting cells, recruiting the Src kinase LCK to the TCR-CD3 complex. LCK phosphorylates substrates, triggering signaling pathways that lead to the production of lymphokines, enhanced motility, adhesion, and activation of cytotoxic T-lymphocytes (CTLs), thereby aiding in the recognition and elimination of infected or tumor cells. Additionally, in natural killer (NK) cells, CD8A homodimers on the cell surface provide a survival mechanism for conjugating with and lysing multiple target cells. CD8A homodimers also facilitate the survival and differentiation of activated lymphocytes into memory CD8 T-cells. This supplementary information is collated from multiple sources and compiled automatically.
See full target information CD8A
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文献 (184)

Recent publications for all applications. Explore the full list and refine your search

Cancers 15: PubMed38136261

2023

Long-Term Survival of Patients with Stage T1N0M1 Renal Cell Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Viktoria Schütz,Huan Lin,Adam Kaczorowski,Stefanie Zschäbitz,Dirk Jäger,Albrecht Stenzinger,Anette Duensing,Jürgen Debus,Markus Hohenfellner,Stefan Duensing

Frontiers in veterinary science 10:1268968 PubMed38046568

2023

Okra [ (L.) Moench] improved blood glucose and restored histopathological alterations in splenic tissues in a rat model with streptozotocin-induced type 1 diabetes through CD8 T cells and NF-kβ expression.

Applications

Unspecified application

Species

Unspecified reactive species

Mohamed A Alblihd,Khalaf F Alsharif,Asmaa A Hamad,Fatma Abo Zakaib Ali,Manal T Hussein,Alaa S Alhegaili,Mohamed Ahmed Hassan,Osama M Al-Amer,Nisreen Khalid Aref Albezrah,Abdulraheem Ali Almalki,Alaa Jameel A Albarakati,Khalid S Alghamdi,Khalid J Alzahrani,Ashraf Albrakati,Elham Hamed Alrubai,Naira ElAshmouny,Ehab Kotb Elmahallawy

iScience 26:108414 PubMed38047083

2023

Helicobacter pylori CagA promotes immune evasion of gastric cancer by upregulating PD-L1 level in exosomes.

Applications

Unspecified application

Species

Unspecified reactive species

Jinfeng Wang,Rilin Deng,Shuai Chen,Shun Deng,Qi Hu,Biaoming Xu,Junjun Li,Zhuo He,Mingjing Peng,Sanlin Lei,Tiexiang Ma,Zhuo Chen,Haizhen Zhu,Chaohui Zuo

Scientific reports 13:17067 PubMed37816950

2023

Use of pediatric thymus to humanize mice for HIV-1 mucosal transmission.

Applications

Unspecified application

Species

Unspecified reactive species

Chandra N Roy,Sherry T Shu,Christopher Kline,Lora Rigatti,Thomas E Smithgall,Zandrea Ambrose

Journal of neurogastroenterology and motility 29:486-500 PubMed37586778

2023

Imbalance of Innate and Adaptive Immunity in Esophageal Achalasia.

Applications

Unspecified application

Species

Unspecified reactive species

Lu Yao,Zuqiang Liu,Weifeng Chen,Jiaqi Xu,Xiaoyue Xu,Jiaxin Xu,Liyun Ma,Xiaoqing Li,Quanlin Li,Pinghong Zhou

Communications biology 6:830 PubMed37563418

2023

Dynamic thresholding and tissue dissociation optimization for CITE-seq identifies differential surface protein abundance in metastatic melanoma.

Applications

Unspecified application

Species

Unspecified reactive species

Ulrike Lischetti,Aizhan Tastanova,Franziska Singer,Linda Grob,Matteo Carrara,Phil F Cheng,Julia M Martínez Gómez,Federica Sella,Veronika Haunerdinger,Christian Beisel,Mitchell P Levesque

Molecular therapy oncolytics 30:103-116 PubMed37635744

2023

Oncolytic virotherapy with intratumoral injection of vaccinia virus TG6002 and 5-fluorocytosine administration in dogs with malignant tumors.

Applications

Unspecified application

Species

Unspecified reactive species

Jérémy Béguin,Eve Laloy,Sandrine Cochin,Murielle Gantzer,Isabelle Farine,Christelle Pichon,Baptiste Moreau,Johann Foloppe,Jean-Marc Balloul,Christelle Machon,Jérôme Guitton,Dominique Tierny,Bernard Klonjkowski,Eric Quéméneur,Christelle Maurey,Philippe Erbs

Journal of neuro-oncology 163:663-674 PubMed37418134

2023

Tumor immune microenvironment in pituitary neuroendocrine tumors (PitNETs): increased M2 macrophage infiltration and PD-L1 expression in PIT1-lineage subset.

Applications

Unspecified application

Species

Unspecified reactive species

Mei Luo,Rui Tang,Haijun Wang

Frontiers in immunology 14:1172768 PubMed37383237

2023

Prognostic impact of intra- and peritumoral immune cell subpopulations in head and neck squamous cell carcinomas - comprehensive analysis of the TCGA-HNSC cohort and immunohistochemical validation on 101 patients.

Applications

Unspecified application

Species

Unspecified reactive species

Moritz Knebel,Sandrina Körner,Jan Philipp Kühn,Silke Wemmert,Lukas Brust,Sigrun Smola,Mathias Wagner,Rainer M Bohle,Luc G T Morris,Abhinav Pandey,Bernhard Schick,Maximilian Linxweiler

BMC gastroenterology 23:194 PubMed37277702

2023

The predictive value of CD4, CD8, and C-reactive protein in the prognosis of schistosomal and non-schistosomal colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Meihong Cheng,Hongyan Jing,Dacheng Bu,Jing Liu,Kui Lu,Jican Liu,Yanchao Xu,Ting Zhu,Yingyong Hou,Junxia Yao,Qilin Zhai,Weixia Wang
View all publications
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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。
保証に関する詳細については利用規約をご確認ください。
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