Anti-CD79a 抗体 [EPR26537-114] (BSA and Azide free) (ab300151)

製品名

Anti-CD79a antibody [EPR26537-114] (BSA and Azide free)
CD79a 一次抗体製品一覧
製品の詳細

Rabbit monoclonal [EPR26537-114] to CD79a - BSA and Azide free
由来種

Rabbit
アプリケーション

適用あり: WB, IHC-P, ICC/IF, IHC-Fr, IPmore details
適用なし: Flow Cyt
種交差性

交差種: Mouse, Rat
非交差種: Human
免疫原

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
ポジティブ･コントロール
- WB: Rat lymph node, Rat spleen, Mouse lymph node, A20, Untreated Rat lymph node and Rat lymph node treated with Protein Deglycosylation MIX II lysates. IHC-P: Mouse spleen, Mouse large B-cell lymphoma, Rat spleen. IHC-Fr: Mouse spleen and Rat spleen tissues. ICC/IF: Mouse splenocytes cells. IP: Mouse lymph node and Rat spleen cells.
特記事項
Ab300151 is a carrier free version of ab300150.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

製品の状態

Liquid
保存方法

Shipped at 4°C. Store at +4°C.
バッファー

pH: 7.2
Constituent: 100% PBS
キャリア・フリー

はい
Concentration information loading...
精製度

Protein A purified
ポリ/モノ

モノクローナル
クローン名

EPR26537-114
アイソタイプ

IgG
研究分野

The Abpromise guarantee

Abpromise保証は、次のテスト済みアプリケーションにおけるab300151の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション	Abreviews	特記事項
WB		Use at an assay dependent concentration. Predicted molecular weight: 25 kDa.
IHC-P		Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF		Use at an assay dependent concentration.
IHC-Fr		Use at an assay dependent concentration.
IP		Use at an assay dependent concentration.

特記事項
WB Use at an assay dependent concentration. Predicted molecular weight: 25 kDa.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
IP Use at an assay dependent concentration.

追加情報

Is unsuitable for Flow Cyt.

機能

Required in cooperation with CD79B for initiation of the signal transduction cascade activated by binding of antigen to the B-cell antigen receptor complex (BCR) which leads to internalization of the complex, trafficking to late endosomes and antigen presentation. Also required for BCR surface expression and for efficient differentiation of pro- and pre-B-cells. Stimulates SYK autophosphorylation and activation. Binds to BLNK, bringing BLNK into proximity with SYK and allowing SYK to phosphorylate BLNK. Also interacts with and increases activity of some Src-family tyrosine kinases. Represses BCR signaling during development of immature B cells.
組織特異性

B-cells.
関連疾患

Defects in CD79A are the cause of agammaglobulinemia type 3 (AGM3) [MIM:613501]. It is a primary immunodeficiency characterized by profoundly low or absent serum antibodies and low or absent circulating B cells due to an early block of B-cell development. Affected individuals develop severe infections in the first years of life. Note=Two different mutations, one at the splice donor site of intron 2 and the other at the splice acceptor site for exon 3, have been identified. Both mutations give rise to a truncated protein.
配列類似性

Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 ITAM domain.
翻訳後修飾

Phosphorylated on tyrosine, serine and threonine residues upon B-cell activation. Phosphorylation of tyrosine residues by Src-family kinases is an early and essential feature of the BCR signaling cascade. The phosphorylated tyrosines serve as docking sites for SH2-domain containing kinases, leading to their activation which in turn leads to phosphorylation of downstream targets. Phosphorylation of serine and threonine residues may prevent subsequent tyrosine phosphorylation.
細胞内局在

Cell membrane. Following antigen binding, the BCR has been shown to translocate from detergent-soluble regions of the cell membrane to lipid rafts although signal transduction through the complex can also occur outside lipid rafts.
Target information above from: UniProt accession P11912 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
参照データベース
- Entrez Gene: 12518 Mouse
- Entrez Gene: 681236 Rat
- SwissProt: P11911 Mouse
- Unigene: 1355 Mouse
別名
- B lymphocyte-specific MB1 protein antibody
- B-cell antigen receptor complex-associated protein alpha chain antibody
- CD 79a antibody
- CD79a antibody
- CD79a antigen (immunoglobulin-associated alpha) antibody
- CD79A antigen antibody
- CD79a molecule, immunoglobulin-associated alpha antibody
- CD79A_HUMAN antibody
- Ig alpha antibody
- Ig-alpha antibody
- IGA antibody
- IgM-alpha antibody
- Immunoglobulin-associated alpha antibody
- Ly54 antibody
- MB-1 membrane glycoprotein antibody
- MB1 antibody
- Membrane-bound immunoglobulin-associated protein antibody
- Surface IgM-associated protein antibody
see all

Western blot - Anti-CD79a antibody [EPR26537-114] (BSA and Azide free) (AB300151)

All lanes : Anti-CD79a antibody [EPR26537-114] (ab300150) at 1/1000 dilution

Lane 1 : Rat lymph node tissue lysate
Lane 2 : Rat spleen tissue lysate
Lane 3 : Rat brain tissue lysate
Lane 4 : Rat skin tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 100000 cells

Predicted band size: 25 kDa
Observed band size: 25, 30-50 kDa why is the actual band size different from the predicted?

Exposure time: 81 seconds

This data was developed using ab300150, the same antibody clone but with different buffer formulation.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Low expression: brain, skin(Human Protein Atlas).
The molecular weight observed is consistent with what has been described in the literature (PMID:15591116).
Western blot - Anti-CD79a antibody [EPR26537-114] (BSA and Azide free) (AB300151)

All lanes : Anti-CD79a antibody [EPR26537-114] (ab300150) at 1/1000 dilution

Lane 1 : Mouse lymph node tissue lysate
Lane 2 : Mouse brain tissue lysate
Lane 3 : Mouse skin tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 25 kDa
Observed band size: 25, 30-50 kDa why is the actual band size different from the predicted?

Exposure time: 81 seconds

This data was developed using ab300150, the same antibody clone but with different buffer formulation.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Low expression: brain, skin(Human Protein Atlas).
The molecular weight observed is consistent with what has been described in the literature (PMID:15591116).
Western blot - Anti-CD79a antibody [EPR26537-114] (BSA and Azide free) (ab300151)

All lanes : Anti-CD79a antibody [EPR26537-114] (ab300150) at 1000 cells

Lane 1 : A20 (Mouse reticulum sarcoma B lymphocyte) whole cell lysate
Lane 2 : C2C12 (Mouse myoblasts myoblast) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Developed using the ECL technique.

Predicted band size: 25 kDa
Observed band size: 25, 30-50 kDa why is the actual band size different from the predicted?

Exposure time: 180 seconds

This data was developed using ab300150, the same antibody clone but with different buffer formulation.

Blocking and dilution buffer and concentration: 5% NFDM/TBST

Low expression: C2C12 (database:Harmonizome).

The molecular weight observed is consistent with what has been described in the literature (PMID: 15591116).

This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.
Western blot - Anti-CD79a antibody [EPR26537-114] (BSA and Azide free) (AB300151)

All lanes : Anti-CD79a antibody [EPR26537-114] (ab300150) at 1/1000 dilution

Lane 2 : Rat lymph node tissue lysate treated with Protein Deglycosylation MIX II

Lysates/proteins at 15 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

Predicted band size: 25 kDa
Observed band size: 25, 30-50 kDa why is the actual band size different from the predicted?

Exposure time: 92 seconds

This data was developed using ab300150, the same antibody clone but with different buffer formulation.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

CD79a is a glycosylated protein and can be deglycosylated by Protein Deglycosylation MIX II.
The molecular weight observed is consistent with what has been described in the literature (PMID:15591116).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD79a antibody [EPR26537-114] (BSA and Azide free) (AB300151)

This data was developed using ab300150, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labelling CD79a with ab300150 at 1/5000 (0.123 µg/ml) followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). Membraneous staining on mouse spleen.The section was incubated with ab300150 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: PBS was used instead of ab300150 followed by ready to use secondary antibody LeicaDS9800 (BOND™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD79a antibody [EPR26537-114] (BSA and Azide free) (AB300151)

This data was developed using ab300150, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse large B-cell lymphoma tissue labelling CD79a with ab300150 at 1/5000 (0.123 µg/ml) followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). Membraneous staining on mouse large B-cell lymphoma. The section was incubated with ab300150 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: PBS was used instead of am300150 followed by ready to use secondary antibody LeicaDS9800 (BOND™ Polymer Refine Detection) was used.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD79a antibody [EPR26537-114] (BSA and Azide free) (AB300151)

This data was developed using ab300150, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Rat spleen tissue labelling CD79a with ab300150 at 1/5000 (0.123 µg/ml) followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection) was used. Membraneous staining on rat spleen.The section was incubated with ab300150 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND^® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control: PBS was used instead of primary antibody (an300150) followed by ready to use secondary antibody LeicaDS9800 (BOND™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD79a antibody [EPR26537-114] (BSA and Azide free) (AB300151)

This data was developed using ab300150, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labelling CD79a with ab300150 at 1/5000 (0.123 µg/ml) followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection) was used. Negative control: no staining on mouse cerebrum. The section was incubated with ab300150 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: PBS was used instead of primary antibody (an300150) followed by ready to use secondary antibody LeicaDS9800 (BOND™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD79a antibody [EPR26537-114] (BSA and Azide free) (AB300151)

This data was developed using ab300150, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labelling CD79a with ab300150 at 1/5000 (0.123 µg/ml) followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection) was used. Negative control: no staining on rat cerebrum.The section was incubated with ab300150 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND^® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control: PBS was used instead of primary antibody (ab300150) followed by ready to use secondary antibody LeicaDS9800 (BOND™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunohistochemistry (Frozen sections) - Anti-CD79a antibody [EPR26537-114] (BSA and Azide free) (AB300151)

This data was developed using ab300150, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse spleen (fresh) tissue labeling CD79a with ab300150 at 1/500 (1.232 µg/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 (2 µg/mL) dilution (Green). Positive staining on mouse spleen is observed. The nuclear counterstain was DAPI (Blue).

Secondary antibody control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbedat 1/1000 (2 µg/mL) dilution.
Immunohistochemistry (Frozen sections) - Anti-CD79a antibody [EPR26537-114] (BSA and Azide free) (AB300151)

This data was developed using ab300150, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat spleen (fresh) tissue labeling CD79a with ab300150 at 1/500 (1.232 µg/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 2 µg/mL dilution (Green). Positive staining on rat spleen is observed. The nuclear counterstain was DAPI (Blue).

Secondary antibody control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbedat 1/1000 (2 µg/mL) dilution.
Immunocytochemistry/ Immunofluorescence - Anti-CD79a antibody [EPR26537-114] (BSA and Azide free) (ab300151)

This data was developed using ab300150, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeilized Mouse splenocytes cells lebelling CD79a with ab300150 at 1/100 (6.16 µg/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed antibody at 1/1000 (2 µg/ml) dilution (Green). Confocal image showing membranous and cytoplasmic staining in subsets of mouse splenocytes. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor^® 594) was used to counterstain tubulin at 1/200 (2.5 µg/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 (2 µg/ml) dilution.
Immunoprecipitation - Anti-CD79a antibody [EPR26537-114] (BSA and Azide free) (AB300151)

This data was developed using ab300150, the same antibody clone in a different buffer formulation.

CD79a was immunoprecipitated from 0.35 mg Mouse lymph node tissue lysate 10 µg with ab300150 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab300150 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1: Mouse lymph node tissue lysate 10 µg

Lane 2: ab300150 IP in Mouse lymph node tissue lysate

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab300150 in Mouse lymph node tissue lysate

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 15 seconds
Immunoprecipitation - Anti-CD79a antibody [EPR26537-114] (BSA and Azide free) (AB300151)

This data was developed using ab300150, the same antibody clone in a different buffer formulation.

CD79a was immunoprecipitated from 0.35 mg rat spleen tissue lysate 10 µg with ab300150 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab300150 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1: Rat spleen tissue lysate 10 µg

Lane 2: ab300150 IP in rat spleen tissue lysate

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab300150 in Rat spleen tissue lysate

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 15 seconds
Anti-CD79a antibody [EPR26537-114] (BSA and Azide free) (AB300151)

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheet download

Download

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ab300151 は論文での使用が確認できていません。

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製品の概要

製品名

製品の詳細

由来種

アプリケーション

種交差性

免疫原

ポジティブ･コントロール

特記事項

製品の特性

製品の状態

保存方法

バッファー

キャリア・フリー

精製度

ポリ/モノ

クローン名

アイソタイプ

研究分野

関連製品

Alternative Versions

Compatible Secondaries

Related Buffer

アプリケーション

The Abpromise guarantee

ターゲット情報

機能

組織特異性

関連疾患

配列類似性

翻訳後修飾

細胞内局在

参照データベース

別名

画像

プロトコール

データシートおよび資料

Datasheet download

Certificate of Compliance

参考文献 (0)

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