Anti-CD75 抗体 [B-L5] (ab27316)
Key features and details
- Mouse monoclonal [B-L5] to CD75
- Suitable for: Flow Cyt, IP
- Reacts with: Human
- Isotype: IgM
製品の概要
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製品名
Anti-CD75 antibody [B-L5]
CD75 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [B-L5] to CD75 -
由来種
Mouse -
特異性
Recognises lactosamines.
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アプリケーション
適用あり: Flow Cyt, IPmore details -
種交差性
交差種: Human -
免疫原
Tissue, cells or virus corresponding to Human CD75. Raji cell line.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.30
Preservative: 0.09% Sodium azide
Constituents: 98.91% PBS, 1% BSA -
Concentration information loading...
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特記事項(精製)
This antibody was purified by gel filtration. -
ポリ/モノ
モノクローナル -
クローン名
B-L5 -
ミエローマ
x63-Ag8.653 -
アイソタイプ
IgM -
軽鎖の種類
kappa -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab27316の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Flow Cyt |
Use 10µl for 106 cells.
ab91545 - Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody.
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IP |
Use a concentration of 5 µg/ml.
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特記事項 |
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Flow Cyt
Use 10µl for 106 cells. ab91545 - Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody.
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IP
Use a concentration of 5 µg/ml. |
ターゲット情報
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機能
Transfers sialic acid from the donor of substrate CMP-sialic acid to galactose containing acceptor substrates. -
パスウェイ
Protein modification; protein glycosylation. -
配列類似性
Belongs to the glycosyltransferase 29 family. -
翻訳後修飾
The soluble form derives from the membrane form by proteolytic processing.
The HB-6, CDW75, and CD76 differentiation antigens are cell-surface carbohydrate determinants generated by this enzyme. -
細胞内局在
Golgi apparatus > Golgi stack membrane. Secreted. Membrane-bound form in trans cisternae of Golgi. Secreted into the body fluid. - Information by UniProt
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参照データベース
- Entrez Gene: 6480 Human
- Omim: 109675 Human
- SwissProt: P15907 Human
- Unigene: 207459 Human
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別名
- 6 sialyltransferase antibody
- 6-sialyltransferase 1 antibody
- 6-ST 1 antibody
see all
画像
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CD75 was immunoprecipitated using 0.5mg HepG2 whole cell extract, 5µg of Mouse monoclonal to CD75 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, HepG2 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab27316.
Secondary: Goat polyclonal to mouse IgG light chain specific (HRP) at 1/20,000 dilution.
Band: 47kDa; CD75
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Ab27316, at a dilution of 10µl for 106 cells or 100 µl of whole blood, staining CD75 by Flow Cytometry.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab27316 は論文での使用が確認できていません。