Anti-CD74 抗体 [In-1] (ab245692)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [In-1] to CD74
- Suitable for: ICC/IF, WB
- Reacts with: Mouse
製品の概要
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製品名
Anti-CD74 antibody [In-1]
CD74 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [In-1] to CD74 -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, WBmore details -
種交差性
交差種: Mouse -
免疫原
Tissue, cells or virus corresponding to CD74. Mouse lymphocytes of strain A injected into Wistar rats.
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ポジティブ・コントロール
- ICC/IF: Mouse splenocytes. WB: Mouse spleen and lymph node lysates.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.02% Proclin 300
Constituent: 99% PBS -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
In-1 -
アイソタイプ
IgG -
軽鎖の種類
kappa -
研究分野
関連製品
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Compatible Secondaries
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab245692の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF |
Use a concentration of 10 µg/ml.
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WB |
Use a concentration of 0.0001 µg/ml. Predicted molecular weight: 35 kDa.
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特記事項 |
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ICC/IF
Use a concentration of 10 µg/ml. |
WB
Use a concentration of 0.0001 µg/ml. Predicted molecular weight: 35 kDa. |
ターゲット情報
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機能
Plays a critical role in MHC class II antigen processing by stabilizing peptide-free class II alpha/beta heterodimers in a complex soon after their synthesis and directing transport of the complex from the endoplasmic reticulum to the endosomal/lysosomal system where the antigen processing and binding of antigenic peptides to MHC class II takes place. Serves as cell surface receptor for the cytokine MIF. -
配列類似性
Contains 1 thyroglobulin type-1 domain. -
細胞内局在
Cell membrane. Endoplasmic reticulum membrane. Golgi apparatus > trans-Golgi network. Endosome. Lysosome. Transits through a number of intracellular compartments in the endocytic pathway. It can either undergo proteolysis or reach the cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 16149 Mouse
- SwissProt: P04441 Mouse
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別名
- CD 74 antibody
- CD74 antibody
- CD74 antigen (invariant polypeptide of major histocompatibility complex, class II antigen-associated) antibody
see all
画像
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PFA fixed, 0.15% Triton permeabilized mouse splenocytes labeling CD74 using ab245692 at 10 µg/ml for 1 hour (green) followed by Alexa Fluor® 488 secondary antibody (1 µg/ml), in ICC/IF. The nuclear counter stain is DAPI (blue).
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All lanes : Anti-CD74 antibody [In-1] (ab245692) at 0.0001 µg/ml (incubated for 1 hr)
Lane 1 : Mouse spleen lysate (RIPA buffer)
Lane 2 : Mouse lymph node lysate (RIPA buffer)
Lysates/proteins at 35 µg per lane.
Predicted band size: 35 kDa10% SDS PAGE gel.
Detected by chemiluminescence.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab245692 は 1 報の論文で使用されています。
- Zhan L et al. A MAC2-positive progenitor-like microglial population is resistant to CSF1R inhibition in adult mouse brain. Elife 9:N/A (2020). PubMed: 33054973