Anti-CD74 抗体 [In-1] (ab245692)
![Immunocytochemistry/ Immunofluorescence - Anti-CD74 antibody [In-1] (ab245692)](https://www.abcam.co.jp/ps/products/245/ab245692/Images/ab245692-336961-anti-cd74-antibody-in-1-immunofluorescence.jpg "Immunocytochemistry/ Immunofluorescence - Anti-CD74 antibody [In-1] (ab245692)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [In-1] to CD74
- Suitable for: ICC/IF, WB
- Reacts with: Mouse
製品の概要
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製品名
Anti-CD74 antibody [In-1]
CD74 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [In-1] to CD74 -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, WBmore details -
種交差性
交差種: Mouse -
免疫原
Tissue, cells or virus corresponding to CD74. Mouse lymphocytes of strain A injected into Wistar rats.
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ポジティブ･コントロール
- ICC/IF: Mouse splenocytes. WB: Mouse spleen and lymph node lysates.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.02% Proclin 300
Constituent: 99% PBS -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
In-1 -
アイソタイプ
IgG -
軽鎖の種類
kappa -
研究分野
関連製品
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Compatible Secondaries
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab245692の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF |
Use a concentration of 10 µg/ml.
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| WB |
Use a concentration of 0.0001 µg/ml. Predicted molecular weight: 35 kDa.
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| 特記事項 |
|---|
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ICC/IF
Use a concentration of 10 µg/ml. |
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WB
Use a concentration of 0.0001 µg/ml. Predicted molecular weight: 35 kDa. |
ターゲット情報
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機能
Plays a critical role in MHC class II antigen processing by stabilizing peptide-free class II alpha/beta heterodimers in a complex soon after their synthesis and directing transport of the complex from the endoplasmic reticulum to the endosomal/lysosomal system where the antigen processing and binding of antigenic peptides to MHC class II takes place. Serves as cell surface receptor for the cytokine MIF. -
配列類似性
Contains 1 thyroglobulin type-1 domain. -
細胞内局在
Cell membrane. Endoplasmic reticulum membrane. Golgi apparatus > trans-Golgi network. Endosome. Lysosome. Transits through a number of intracellular compartments in the endocytic pathway. It can either undergo proteolysis or reach the cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 16149 Mouse
- SwissProt: P04441 Mouse
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別名
- CD 74 antibody
- CD74 antibody
- CD74 antigen (invariant polypeptide of major histocompatibility complex, class II antigen-associated) antibody
see all
画像
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Immunocytochemistry/ Immunofluorescence - Anti-CD74 antibody [In-1] (ab245692)
PFA fixed, 0.15% Triton permeabilized mouse splenocytes labeling CD74 using ab245692 at 10 µg/ml for 1 hour (green) followed by Alexa Fluor® 488 secondary antibody (1 µg/ml), in ICC/IF. The nuclear counter stain is DAPI (blue).
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![Western blot - Anti-CD74 antibody [In-1] (ab245692)](https://www.abcam.co.jp/ps/products/245/ab245692/Images/ab245692-336960-anti-cd74-antibody-in-1-western-blot.jpg)
Western blot - Anti-CD74 antibody [In-1] (ab245692)All lanes : Anti-CD74 antibody [In-1] (ab245692) at 0.0001 µg/ml (incubated for 1 hr)
Lane 1 : Mouse spleen lysate (RIPA buffer)
Lane 2 : Mouse lymph node lysate (RIPA buffer)
Lysates/proteins at 35 µg per lane.
Predicted band size: 35 kDa10% SDS PAGE gel.
Detected by chemiluminescence.
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![Anti-CD74 antibody [In-1] (ab245692)](https://www.abcam.co.jp/ps/products/245/ab245692/Images/ab245692-3-benefits-of-recombinant-antibodies.png)
Anti-CD74 antibody [In-1] (ab245692)
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab245692 は 1 報の論文で使用されています。
- Zhan L et al. A MAC2-positive progenitor-like microglial population is resistant to CSF1R inhibition in adult mouse brain. Elife 9:N/A (2020). PubMed: 33054973