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AB245692

Anti-CD74 抗体 [In-1]

Anti-CD74 antibody [In-1]

5

(1 Review)

|

(6 Publications)

Rabbit Recombinant Monoclonal CD74 antibody. Suitable for WB, ICC/IF and reacts with Mouse samples. Cited in 6 publications. Immunogen corresponding to Cell preparation containing Cd74 protein.

別名を表示する

CD74, Ii, Cd74, H-2 class II histocompatibility antigen gamma chain, Ia antigen-associated invariant chain, MHC class II-associated invariant chain, Ii chain

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-CD74 antibody [In-1] (AB245692)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CD74 antibody [In-1] (AB245692)

PFA fixed, 0.15% Triton permeabilized mouse splenocytes labeling CD74 using ab245692 at 10 μg/ml for 1 hour (green) followed by Alexa Fluor® 488 secondary antibody (1 μg/ml), in ICC/IF. The nuclear counter stain is DAPI (blue).

Western blot - Anti-CD74 antibody [In-1] (AB245692)
  • WB

Supplier Data

Western blot - Anti-CD74 antibody [In-1] (AB245692)

10% SDS PAGE gel.

Detected by chemiluminescence.

All lanes:

Western blot - Anti-CD74 antibody [In-1] (ab245692) at 0.0001 µg/mL

Lane 1:

Mouse spleen lysate (RIPA buffer) at 35 µg

Lane 2:

Mouse lymph node lysate (RIPA buffer) at 35 µg

Predicted band size: 34 kDa

false

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

In-1

アイソタイプ

IgG

軽鎖のタイプ

kappa

キャリアフリー

No

交差種

Mouse

アプリケーション

ICC/IF, WB

applications

免疫原

Cell preparation containing Cd74 protein. The exact immunogen used to generate this antibody is proprietary information.

P04441

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.0001 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "10 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
Preservative: 0.02% Proclin 300 Constituents: PBS
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

CD74 also known as the invariant chain or li is a protein with a mass of about 33 kDa. It is widely expressed on the surface of immune cells such as B cells dendritic cells and macrophages. CD74 plays an important role in the immune system by acting as a chaperone for major histocompatibility complex class II (MHC II) molecules guiding their migration to endosomes where they encounter antigenic peptides. The CD74 protein also functions as a cell surface receptor for macrophage migration inhibitory factor (MIF) enhancing the immune response process.
Biological function summary

CD74 facilitates multiple immune regulatory processes. It forms a complex with the MHC II molecules aiding in their proper folding and peptide loading in the endoplasmic reticulum. This complex then travels through the Golgi apparatus towards the lysosomal compartments where antigens are presented to initiate adaptive immune responses. In addition CD74 is involved in signal transduction pathways that regulate cell proliferation and survival influenced by interactions with MIF and other molecules.

Pathways

The role of CD74 extends into both the antigen presentation and MIF signaling pathways. Within the antigen presentation pathway CD74's activity is tightly connected with MHC II and subsequently to CD4+ T cells facilitating the activation of these immune cells. The MIF signaling pathway involves CD74 interacting with CD44 a receptor that further propagates the signaling cascades essential for immune modulation and cell survival.

CD74 is linked to inflammatory and autoimmune conditions such as rheumatoid arthritis and systemic lupus erythematosus. Aberrant expression of CD74 influences the presentation of autoantigens and may contribute to the chronic inflammation seen in these disorders. Its connection to MIF which is a pro-inflammatory cytokine further implicates CD74 in these autoimmune scenarios by promoting persistent immune activation. Researchers are also exploring CD74 as a therapeutic target in certain cancers due to its influence on immune surveillance and tumor progression.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Plays a critical role in MHC class II antigen processing by stabilizing peptide-free class II alpha/beta heterodimers in a complex soon after their synthesis and directing transport of the complex from the endoplasmic reticulum to compartments where peptide loading of class II takes place. Enhance also the stimulation of T-cell responses through interaction with CD44.. Isoform Long. Stabilizes the conformation of mature CTSL by binding to its active site and serving as a chaperone to help maintain a pool of mature enzyme in endocytic compartments and extracellular space of antigen-presenting cells (APCs).. Class-II-associated invariant chain peptide. Binds to the peptide-binding site of MHC class II alpha/beta heterodimers forming an alpha-beta-CLIP complex, thereby preventing the loading of antigenic peptides to the MHC class II complex until its release by HLA-DM in the endosome.
See full target information Cd74
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文献 (6)

Recent publications for all applications. Explore the full list and refine your search

iScience 27:110735 PubMed39280609

2024

Single-cell RNA-seq reveals a resolving immune phenotype in the oral mucosa.

Applications

Unspecified application

Species

Unspecified reactive species

Paul Cantalupo,Alex Diacou,Sangmin Park,Vishal Soman,Jiamiao Chen,Deshawna Glenn,Uma Chandran,Daniel Clark

Aging cell 23:e14172 PubMed38747044

2024

Exercise rejuvenates microglia and reverses T cell accumulation in the aged female mouse brain.

Applications

Unspecified application

Species

Unspecified reactive species

Solal Chauquet,Emily F Willis,Laura Grice,Samuel B R Harley,Joseph E Powell,Naomi R Wray,Quan Nguyen,Marc J Ruitenberg,Sonia Shah,Jana Vukovic

BMC oral health 24:407 PubMed38556862

2024

KAT2A-mediated succinylation modification of notch1 promotes the proliferation and differentiation of dental pulp stem cells by activating notch pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Longwei Ye,Zeqin Yu,Lin He,Jie Yuan,Xiaodan Zhang,Lei Li,Xin Huang,Yanyan Ma,Lei Zhang

eLife 12: PubMed37057892

2023

MHC class I and MHC class II reporter mice enable analysis of immune oligodendroglia in mouse models of multiple sclerosis.

Applications

Unspecified application

Species

Unspecified reactive species

Em P Harrington,Riley B Catenacci,Matthew D Smith,Dongeun Heo,Cecilia E Miller,Keya R Meyers,Jenna Glatzer,Dwight E Bergles,Peter A Calabresi

Nature neuroscience 26:416-429 PubMed36635496

2023

A TREM2-activating antibody with a blood-brain barrier transport vehicle enhances microglial metabolism in Alzheimer's disease models.

Applications

Unspecified application

Species

Unspecified reactive species

Bettina van Lengerich,Lihong Zhan,Dan Xia,Darren Chan,David Joy,Joshua I Park,David Tatarakis,Meredith Calvert,Selina Hummel,Steve Lianoglou,Michelle E Pizzo,Rachel Prorok,Elliot Thomsen,Laura M Bartos,Philipp Beumers,Anja Capell,Sonnet S Davis,Lis de Weerd,Jason C Dugas,Joseph Duque,Timothy Earr,Kapil Gadkar,Tina Giese,Audrey Gill,Johannes Gnörich,Connie Ha,Malavika Kannuswamy,Do Jin Kim,Sebastian T Kunte,Lea H Kunze,Diana Lac,Kendra Lechtenberg,Amy Wing-Sze Leung,Chun-Chi Liang,Isabel Lopez,Paul McQuade,Anuja Modi,Vanessa O Torres,Hoang N Nguyen,Ida Pesämaa,Nicholas Propson,Marvin Reich,Yaneth Robles-Colmenares,Kai Schlepckow,Luna Slemann,Hilda Solanoy,Jung H Suh,Robert G Thorne,Chandler Vieira,Karin Wind-Mark,Ken Xiong,Y Joy Yu Zuchero,Dolo Diaz,Mark S Dennis,Fen Huang,Kimberly Scearce-Levie,Ryan J Watts,Christian Haass,Joseph W Lewcock,Gilbert Di Paolo,Matthias Brendel,Pascal E Sanchez,Kathryn M Monroe

eLife 9: PubMed33054973

2020

A MAC2-positive progenitor-like microglial population is resistant to CSF1R inhibition in adult mouse brain.

Applications

Unspecified application

Species

Unspecified reactive species

Lihong Zhan,Li Fan,Lay Kodama,Peter Dongmin Sohn,Man Ying Wong,Gergey Alzaem Mousa,Yungui Zhou,Yaqiao Li,Li Gan
View all publications
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