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  1. Link

    products/primary-antibodies/cd73-antibody-7g2-ab54217.pdf

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Cardiovascular Hypoxia Associated Proteins
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Anti-CD73 抗体 [7G2] (ab54217)

  • Datasheet
  • SDS
Reviews (3)Q&A (11)References (30)

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Abpromise

保証された製品品質、優れたカスタマー・サポート。 詳細はこちら。

Immunocytochemistry - Anti-CD73 antibody [7G2] (ab54217)
  • Flow Cytometry - Anti-CD73 antibody [7G2] (ab54217)

Key features and details

  • Mouse monoclonal [7G2] to CD73
  • Suitable for: Flow Cyt, ICC
  • Reacts with: Human
  • Isotype: IgG2a

こちらの製品もご検討ください

一次抗体
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Anti-CD105 antibody [EPR10145-12] (ab169545)
標識
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Alexa Fluor® 488 Conjugation Kit (Fast) - Lightning-Link® (ab236553)
一次抗体
Product image
Anti-CD90 / Thy1 antibody [EPR28145-53] (ab307736)

関連製品

製品の概要

  • 製品名

    Anti-CD73 antibody [7G2]
    CD73 一次抗体 製品一覧
  • 製品の詳細

    Mouse monoclonal [7G2] to CD73
  • 由来種

    Mouse
  • アプリケーション

    適用あり: Flow Cyt, ICCmore details
    適用なし: WB
  • 種交差性

    交差種: Human
  • 免疫原

    Full length purified Human placental CD73.

  • ポジティブ・コントロール

    • Flow Cyt: Human peripheral blood lymphocytes. ICC: U-87 MG cells.
  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • バッファー

    pH: 7.40
    Preservative: 0.1% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • 精製度

    Protein A purified
  • ポリ/モノ

    モノクローナル
  • クローン名

    7G2
  • アイソタイプ

    IgG2a
  • 軽鎖の種類

    kappa
  • 研究分野

    • Cardiovascular
    • Hypoxia
    • Associated Proteins
    • Cardiovascular
    • Atherosclerosis
    • Vascular Inflammation
    • Leukocyte recruitment
    • Other

関連製品

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Mouse IgG2a, kappa monoclonal [MG2a-53] - Isotype control (ab18415)
  • Recombinant Protein

    • Recombinant mouse CD73 protein (His tag) (ab223023)

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab54217の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt (1)
Use a concentration of 1 - 2 µg/ml.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

ICC (1)
1/100.
特記事項
Flow Cyt
Use a concentration of 1 - 2 µg/ml.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

ICC
1/100.
追加情報
Is unsuitable for WB.

ターゲット情報

  • 機能

    Hydrolyzes extracellular nucleotides into membrane permeable nucleosides.
  • 関連疾患

    Defects in NT5E are the cause of calcification of joints and arteries (CAJA) [MIM:211800]. A condition characterized by adult-onset calcification of the lower extremity arteries, including the iliac, femoral and tibial arteries, and hand and foot capsule joints. Age of onset has been reported as early as the second decade of life, usually involving intense joint pain or calcification in the hands.
  • 配列類似性

    Belongs to the 5'-nucleotidase family.
  • 細胞内局在

    Cell membrane.
  • Target information above from: UniProt accession P21589 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 4907 Human
    • Omim: 129190 Human
    • SwissProt: P21589 Human
    • Unigene: 153952 Human
    • 別名

      • 5' NT antibody
      • 5' nucleotidase (CD73) antibody
      • 5' nucleotidase precursor antibody
      • 5' nucleotidase, ecto antibody
      • 5' nucleotidase, ecto (CD73) antibody
      • 5'-NT antibody
      • 5'-nucleotidase antibody
      • 5NTD_HUMAN antibody
      • CD73 antibody
      • CD73 antigen antibody
      • E5NT antibody
      • Ecto 5' nucleotidase antibody
      • Ecto-5'-nucleotidase antibody
      • eN antibody
      • eNT antibody
      • NT antibody
      • NT5 antibody
      • NT5E antibody
      • NTE antibody
      • Purine 5 Prime Nucleotidase antibody
      see all

    画像

    • Immunocytochemistry - Anti-CD73 antibody [7G2] (ab54217)
      Immunocytochemistry - Anti-CD73 antibody [7G2] (ab54217)
      Immunofluorescence analysis of CD73 was performed using 70% confluent log phase U-87 MG cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with ab54217 at a concentration of 1 µg/mL in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with a Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 conjugate, (1:2000 dilution), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b: Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (1:300 dilution). Panel d represents the merged image showing membrane localization. Panel e represents HeLa cells showing no expression of CD73. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

       

    • Flow Cytometry - Anti-CD73 antibody [7G2] (ab54217)
      Flow Cytometry - Anti-CD73 antibody [7G2] (ab54217)
      ab54217 staining human peripheral blood lymphocytes by FACS analysis.

    プロトコール

    • Flow cytometry protocols
    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    データシートおよび資料

    • SDS download

    • Datasheet download

      Download

    参考文献 (30)

    ab54217 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab54217 は 30 報の論文で使用されています。

    • Zhang Y  et al. Definitive Endodermal Cells Supply an in vitro Source of Mesenchymal Stem/Stromal Cells. Commun Biol 6:476 (2023). PubMed: 37127734
    • Perez JE  et al. Modulated nanowire scaffold for highly efficient differentiation of mesenchymal stem cells. J Nanobiotechnology 20:282 (2022). PubMed: 35710420
    • Sikora M  et al. Bone marrow stromal cells (BMSCs CD45- /CD44+ /CD73+ /CD90+ ) isolated from osteoporotic mice SAM/P6 as a novel model for osteoporosis investigation. J Cell Mol Med 25:6634-6651 (2021). PubMed: 34075722
    • Russo E  et al. Energy Metabolism Analysis of Three Different Mesenchymal Stem Cell Populations of Umbilical Cord Under Normal and Pathologic Conditions. Stem Cell Rev Rep 16:585-595 (2020). PubMed: 32185666
    • Ou X  et al. Activation of SIRT1 promotes cartilage differentiation and reduces apoptosis of nucleus pulposus mesenchymal stem cells via the MCP1/CCR2 axis in subjects with intervertebral disc degeneration. Int J Mol Med 46:1074-1084 (2020). PubMed: 32705163
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-10 of 14 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-CD73 antibody [7G2]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Horse Cell (Adipose and bone marrow stem cells)
    Specification
    Adipose and bone marrow stem cells
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - TRITON x100 0,1%
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 37°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    MS. Vega Villar

    Verified customer

    投稿 Mar 29 2012

    Question


    Je suis intéressé d’avoir l’hybridome correspondant à l’anticorps anti-CD73 humain clone 7G2 (n° cat ab54217). Pouvez vous s’il vous plait me dire si vous vendez cet hybridome?

    Merci d’avance

    Read More

    Abcam community

    Verified customer

    Asked on Oct 17 2012

    Answer

    Merci de nous avoir contactés.

    Malheureusement, nous n'avons pas d’hybridome dans notre catalogue.

    Je vous recommande de regarder sur le site Web Biocompare qui vous permettra de rechercher des d'anticorps et leurs fournisseurs. Le lien est le suivant:
    http://www.biocompare.com

    J'espère que ces informations vous seront utiles. N'hésitez pas à nous contacter de nouveau si vous avez d'autres questions.

    Read More

    Abcam Scientific Support

    Answered on Oct 17 2012

    Question

    Our customer purchased ab54217 to test IHC-Fr.
    It mentioned dilution ratio is 1-5mg/ml on the datasheet and I think it is too much volume.
    Could you explain this issue?
    Please check attached file.
    Best regards

    Read More

    Abcam community

    Verified customer

    Asked on Jul 31 2012

    Answer

    Thank you for contacting us.

    We apologize for this error. The concentration should be read as 1-5ug/ml; the datasheet has been updated.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    Abcam Scientific Support

    Answered on Jul 31 2012

    Question

    Could you please refund the money? We try to get this antibody from another company. Do you need any further information for refunding the money?

    Thank you for the information.

    Read More

    Abcam community

    Verified customer

    Asked on Jun 25 2012

    Answer

    Thank you for your reply.

    I have asked our Finance department to issue a credit note for you. The credit note may be used in one of the following ways:
    (1) Redeemed against the original invoice if this hasn't already been paid.
    (2) Held on the account for use against a future order.
    (3) A full refund can be offered where no other invoices are outstanding.

    To specifically receive a refund please ask your Finance department to contact our Finance department at creditcontrol@abcam.com or by telephone using the information at the “Contact Us” link in the top right corner of our website.

    I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service should you require further expert advice

    Read More

    Abcam Scientific Support

    Answered on Jun 25 2012

    Question

    Is there another batch of this product available?

    Read More

    Abcam community

    Verified customer

    Asked on Jun 06 2012

    Answer

    Thank you for contacting us.

    Apologies for the delay in getting back to you. Unfortunately we do not have another batch of this product available. As I mentioned previously I would be happy to refund the item or issue a free unit of another product.

    Apologies for any inconvenience caused.

    Read More

    Abcam Scientific Support

    Answered on Jun 06 2012

    Question

    We received a new antiboday from the same batch and different lot. This did not work. We would like to have a new antibody from a different batch.

    Read More

    Abcam community

    Verified customer

    Asked on Jun 01 2012

    Answer

    I am sorry for the delay in responding to your request for another batch of ab54217.

    I have been in contact with the lab and unfortunately there is not another lot available. I would be happy to refund the item or issue a free unit of another product. Please do let me know how you would like to proceed.

    Looking forward to your reply.

    Read More

    Abcam Scientific Support

    Answered on Jun 01 2012

    Question

    The FOCR did not work

    Read More

    Abcam community

    Verified customer

    Asked on May 15 2012

    Answer

    Thank you for responding to our survey.
    I am sorry to hear the replacement vial did not work as expected. I am currently in contact with the lab to identify whether another batch is available. I will be back in touch as soon as I can. If there is anything I can do in the meantime, please just let me know.
    Thank you for your patience.

    Read More

    Abcam Scientific Support

    Answered on May 15 2012

    Question

    Thank you very much for your reply, as well as your help. The antibodies chosen are in the table below.
    Antibody
    Ab Cat #Mouse monoclonal [SN6] to CD105 (FITC) Ab11415
    Mouse monoclonal [F15-42-1] to CD90 / Thy1 (FITC)Ab11155
    Many thanks for your reply
    Regards

    Read More

    Abcam community

    Verified customer

    Asked on Apr 20 2012

    Answer

    DISCOUNT CODE: XXXXXXXXXXXXXXX
    Expiration date: 18-08-2012

    DISCOUNT CODE: XXXXXXXXXXXXXXX
    Expiration date: 18-08-2012

    I am very pleased to hear you would like to accept our offer and test ab11415 and ab11155in [Sheep]. This code will give you: 1 free [PRIMARY ANTIBODY/PROTEIN] OR VALUE OFF OF ORDER before the expiration date. To redeem this offer, please submit an Abreview for [Sheep] and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

    Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

    Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

    The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

    Read More

    Abcam Scientific Support

    Answered on Apr 20 2012

    Question

    Looking for an anti-CD73 with neutralising properties.

    Read More

    Abcam community

    Verified customer

    Asked on Mar 28 2012

    Answer

    Merci de nous avoir contactés et désolé pour le délai de ma réponse.
    Je viens d'être informé que l'anti-CD73 clone [7G2] (référence ab54217) a été décrit pour des tests d'inhibition dans la publication suivante : Thomson LF, et al. Tissue Antigens 35(1):9-19, 1990. Je n'ai malheureusement pas accès à cet article et ne peut donc pas vous donner plus de détails sur le protocole utilisé.
    Nous ne fournissons pas cette référence sans conservateur dans le tampon.
    J'espère que ces informations vous seront utiles. N'hésitez pas à nous contacter de nouveau si vous avez d'autres questions.

    Read More

    Abcam Scientific Support

    Answered on Mar 28 2012

    Question

    Dear Sirs,

    Can you suggest consitutions for the following ab’s

    AB11414 - Mouse monoclonal [SN6] to CD105

    AB54217 - Mouse monoclonal [7G2] to CD73

    AB49492 - Mouse monoclonal [N1238] to CD146

    Very best regards,

    Read More

    Abcam community

    Verified customer

    Asked on Mar 28 2012

    Answer

    Thank you for your reply.

    For dilution of the antibodies ready to use in the experiment, as discussed in the previous email I can suggest to dilute antibodiesin PBS orTBS. It is sometimes helpful to include 0.2% Tween 20, however this may need to be optimized individually. If there is high background staining, then 2 to 5% blocking agent can also be included.

    You may like to consider reviewing our online protocols from our Scientific support protocols and troubleshooting pages. IHC documents are under Immunostaining. These will contain some further information that I hope may be helpful:

    https://www.abcam.com/index.html?pageconfig=popular_protocols

    Should you have any further questions, please do not hesitate to contact us.

    Read More

    Abcam Scientific Support

    Answered on Mar 28 2012

    1-10 of 14 Abreviews or Q&A

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    For licensing inquiries, please contact partnerships@abcam.com

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