Anti-CD73 抗体 [7G2] (ab54217)
Key features and details
- Mouse monoclonal [7G2] to CD73
- Suitable for: Flow Cyt, ICC
- Reacts with: Human
- Isotype: IgG2a
製品の概要
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製品名
Anti-CD73 antibody [7G2]
CD73 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [7G2] to CD73 -
由来種
Mouse -
アプリケーション
適用あり: Flow Cyt, ICCmore details
適用なし: WB -
種交差性
交差種: Human -
免疫原
Full length purified Human placental CD73.
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ポジティブ・コントロール
- Flow Cyt: Human peripheral blood lymphocytes. ICC: U-87 MG cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
バッファー
pH: 7.40
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
7G2 -
アイソタイプ
IgG2a -
軽鎖の種類
kappa -
研究分野
関連製品
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab54217の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Flow Cyt | (1) |
Use a concentration of 1 - 2 µg/ml.
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
ICC | (1) |
1/100.
|
特記事項 |
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Flow Cyt
Use a concentration of 1 - 2 µg/ml. ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
ICC
1/100. |
ターゲット情報
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機能
Hydrolyzes extracellular nucleotides into membrane permeable nucleosides. -
関連疾患
Defects in NT5E are the cause of calcification of joints and arteries (CAJA) [MIM:211800]. A condition characterized by adult-onset calcification of the lower extremity arteries, including the iliac, femoral and tibial arteries, and hand and foot capsule joints. Age of onset has been reported as early as the second decade of life, usually involving intense joint pain or calcification in the hands. -
配列類似性
Belongs to the 5'-nucleotidase family. -
細胞内局在
Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 4907 Human
- Omim: 129190 Human
- SwissProt: P21589 Human
- Unigene: 153952 Human
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別名
- 5' NT antibody
- 5' nucleotidase (CD73) antibody
- 5' nucleotidase precursor antibody
see all
画像
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Immunofluorescence analysis of CD73 was performed using 70% confluent log phase U-87 MG cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with ab54217 at a concentration of 1 µg/mL in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with a Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 conjugate, (1:2000 dilution), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b: Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (1:300 dilution). Panel d represents the merged image showing membrane localization. Panel e represents HeLa cells showing no expression of CD73. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.
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ab54217 staining human peripheral blood lymphocytes by FACS analysis.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (30)
ab54217 は 30 報の論文で使用されています。
- Zhang Y et al. Definitive Endodermal Cells Supply an in vitro Source of Mesenchymal Stem/Stromal Cells. Commun Biol 6:476 (2023). PubMed: 37127734
- Perez JE et al. Modulated nanowire scaffold for highly efficient differentiation of mesenchymal stem cells. J Nanobiotechnology 20:282 (2022). PubMed: 35710420
- Sikora M et al. Bone marrow stromal cells (BMSCs CD45- /CD44+ /CD73+ /CD90+ ) isolated from osteoporotic mice SAM/P6 as a novel model for osteoporosis investigation. J Cell Mol Med 25:6634-6651 (2021). PubMed: 34075722
- Russo E et al. Energy Metabolism Analysis of Three Different Mesenchymal Stem Cell Populations of Umbilical Cord Under Normal and Pathologic Conditions. Stem Cell Rev Rep 16:585-595 (2020). PubMed: 32185666
- Ou X et al. Activation of SIRT1 promotes cartilage differentiation and reduces apoptosis of nucleus pulposus mesenchymal stem cells via the MCP1/CCR2 axis in subjects with intervertebral disc degeneration. Int J Mol Med 46:1074-1084 (2020). PubMed: 32705163