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Anti-CD6/T12 抗体 [MEM-98] (ab9181)

Submit a review Submit a question References (2)
Western blot - Anti-CD6/T12 antibody [MEM-98] (ab9181)
  • Flow Cytometry - Anti-CD6/T12 antibody [MEM-98] (ab9181)
  • Flow Cytometry - Anti-CD6/T12 antibody [MEM-98] (ab9181)

Key features and details

  • Mouse monoclonal [MEM-98] to CD6/T12
  • Suitable for: WB, Flow Cyt
  • Reacts with: Human
  • Isotype: IgG1

製品の概要

  • 製品名

    Anti-CD6/T12 antibody [MEM-98]
    CD6/T12 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [MEM-98] to CD6/T12

  • 由来種

    Mouse

  • 特異性

    CD6 / T12 antigen (human)

  • アプリケーション

    適用あり: WB, Flow Cytmore details

  • 種交差性

    交差種: Human

  • 免疫原

    Full length native protein (purified) corresponding to Human CD6/T12.

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab9181の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 72 kDa.

works in WB application under non-reducing conditions
Flow Cyt
Use a concentration of 2 µg/ml.
特記事項
WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 72 kDa.

works in WB application under non-reducing conditions
Flow Cyt
Use a concentration of 2 µg/ml.

ターゲット情報

  • 機能

    Involved in cell adhesion. Binds to CD166.

  • 組織特異性

    Expressed by thymocytes, mature T-cells, a subset of B-cells known as B-1 cells, and by some cells in the brain.

  • 配列類似性

    Contains 3 SRCR domains.

  • 翻訳後修飾

    After T-cell activation, becomes hyperphosphorylated on Ser and Thr residues and phosphorylated on Tyr residues.
    Contains intrachain disulfide bond(s).

  • 細胞内局在

    Membrane.
  • Information by UniProt

  • 参照データベース

    • 別名

      • CD_antigen=CD6 antibody
      • CD6 antibody
      • CD6 antigen Tp120 antibody
      • CD6 molecule antibody
      • CD6_HUMAN antibody
      • FLJ44171 antibody
      • OX52 antibody
      • T cell differentiation antigen CD6 antibody
      • T-cell differentiation antigen CD6 antibody
      • T12 antibody
      • TP120 antibody
      see all

    画像

    • Western blot - Anti-CD6/T12 antibody [MEM-98] (ab9181)
      Western blot - Anti-CD6/T12 antibody [MEM-98] (ab9181)
      All lanes : Anti-CD6/T12 antibody [MEM-98] (ab9181) at 1 µg/ml

      Lane 1 : Molt-4 whole cell extracts, reducing
      Lane 2 : HeLa whole cell extracts, reducing
      Lane 3 : Molt-4 whole cell extracts, non-reducing
      Lane 4 : HeLa whole cell extracts, non-reducing

      Secondary
      All lanes : IRDye 800CW Goat-anti-Mouse IgG

      Predicted band size: 72 kDa



      Nitrocellulose membrane blot was probed with ab9181 used at 1 µg/ml, followed by IRDye 800CW Goat-anti-Mouse IgG (green). Mouse anti-GAPDH monoclonal antibody conjugated with DyLight 680 (0.1 µg/ml), was used as the loading control (red). Multiplex fluorescent Western blot detection was performed.

      CD6 molecules were detected at ~100 kDa in Molt-4 cell line under non-reducing conditions.

    • Flow Cytometry - Anti-CD6/T12 antibody [MEM-98] (ab9181)
      Flow Cytometry - Anti-CD6/T12 antibody [MEM-98] (ab9181)

      Flow cytometric analysis of human CD6 positive lymphocytes (red-filled) / neutrophil granulocytes (black-dashed) from peripheral whole blood cells labeling CD6/T12 with ab9181 at 2µg/ml. Secondary used is a goat anti-mouse-APC.

    • Flow Cytometry - Anti-CD6/T12 antibody [MEM-98] (ab9181)
      Flow Cytometry - Anti-CD6/T12 antibody [MEM-98] (ab9181)

      Flow cytometric analysis of human peripheral whole blood labeling CD6/T12 with ab9181 at 2µg/ml. Secondary used is a goat anti-mouse-APC.

    参考文献 (2)

    ab9181 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab9181 は 2 報の論文で使用されています。

    • Zhao L  et al. Expression level of NEAT1 differentiates benign and malignant thyroid nodules by regulating NEAT1/miR-9/PTEN and NEAT1/miR-124/PDCD6 signalling. Int J Mol Med 46:1661-1670 (2020). PubMed: 32901835
    • Bazil V  et al. Monoclonal antibodies against human leucocyte antigens. III. Antibodies against CD45R, CD6, CD44 and two newly described broadly expressed glycoproteins MEM-53 and MEM-102. Folia Biol (Praha) 35:289-97 (1989). PubMed: 2534480

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