AB31630

Anti-CD68 抗体 [ED1]

Name: Anti-CD68 抗体 [ED1] (ab31630) | Abcam
Brand: Abcam Limited
SKU: AB31630
Price: 57000 JPY
Availability: InStock
Rating: 5 (21 reviews)

Anti-CD68 antibody [ED1]

Lab Essentials
詳細を見る

(21 Reviews)

(490 Publications)

Anti-CD68 antibody [ED1] (ab31630) is a mouse monoclonal antibody detecting CD68 in Flow Cytometry (Intra). Suitable for Rat.

- Over 400 publications
- Trusted since 2006

別名を表示する

CD68 antigen, CD68 molecule, CD68_HUMAN, DKFZp686M18236, GP11, Gp110, LAMP4, MACROPHAGE ANTIGEN CD68, Macrophage antigen CD68 (microsialin), Macrosialin, SCARD1, Scavenger receptor class D member 1

2 Images

Flow Cytometry (Intracellular) - Anti-CD68 antibody [ED1] (AB31630)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-CD68 antibody [ED1] (AB31630)

Staining of rat peritoneal macrophages with Alexa Fluor^®647 conjugated mouse anti rat CD68, clone ED1 (ab31630, red filled histogram) following permeabilization with Leucoperm.

Flow Cytometry - Anti-CD68 antibody [ED1] (AB31630)

Flow Cyt

CiteAb

Flow Cytometry - Anti-CD68 antibody [ED1] (AB31630)

CD68 flow cytometry using Anti-CD68 antibody [ED1] ab31630. IPublication image and figure legend from Xia, Z., Wang, J., et al., 2021, Braz J Med Biol Res, PubMed 34320121.

Emodin modulated in vivo M1 and M2 polarization of macrophages in implanted polyvinyl alcohol (PVA) sponges. A-D, The implanted PVA sponges were removed and assessed for M1 (A and C) and M2 (B and D) populations at 3 days (A and B) and 7 days (C and D) after implantation. The percentages of M1 and M2 macrophages and total macrophages in sponge exudates were determined by flow cytometry using the indicated markers. Representative flow profiles are shown (left), and the percentages of M1/M2 populations are summarized (right). Data are reported as means±SE for n=3 for each group. *P,0.05, **P<0.01, ***P<0.001 vs the Control group (two-tailed t-test).

Key facts

宿主種

Mouse

クローン性

Monoclonal

クローン番号

ED1

アイソタイプ

IgG1

キャリアフリー

交差種

Rat

アプリケーション

Flow Cyt (Intra)

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

特異性

ab31630 recognises a single chain glycoprotein of 110kD that is expressed predominantly on the lysosomal membrane of myeloid cells. Weak cell surface expression also occurs. The antigen is expressed by the majority of tissue macrophages and weakly by peripheral blood granulocytes. Studies have shown that the antigen recognised by ED1 is the rat homologue of human CD68.

style

left-column

代替製品

Primary Antibodies

AB53444

Anti-CD68 antibody [FA-11]

Lab Essentials|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-CD68 antibody [FA-11] (AB53444)

Primary Antibodies

AB233172

Anti-CD68 antibody [KP1] - BSA and Azide free

Advanced Validation|Recombinant

Mass Cytometry - Anti-CD68 antibody [KP1] - BSA and Azide free (AB233172)

Primary Antibodies

AB222914

Alexa Fluor® 488 Anti-CD68 antibody [KP1]

Recombinant|Advanced Validation

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-CD68 antibody [KP1] (AB222914)

Primary Antibodies

AB277938

Alexa Fluor® 594 Anti-CD68 antibody [EPR20545]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 594 Anti-CD68 antibody [EPR20545] (AB277938)

Primary Antibodies

AB224029

Alexa Fluor® 647 Anti-CD68 antibody [EPR20545]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-CD68 antibody [EPR20545] (AB224029)

Primary Antibodies

AB201844

Alexa Fluor® 488 Anti-CD68 antibody [FA-11]

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-CD68 antibody [FA-11] (AB201844)

Primary Antibodies

AB201845

Alexa Fluor® 647 Anti-CD68 antibody [FA-11]

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-CD68 antibody [FA-11] (AB201845)

Primary Antibodies

AB227458

Anti-CD68 antibody [EPR20545] - BSA and Azide free

BOND RX™ Validated|RabMAb|Advanced Validation|Recombinant

Multiplex immunohistochemistry - Anti-CD68 antibody [EPR20545] - BSA and Azide free (AB227458)

Primary Antibodies

AB125212

Anti-CD68 antibody

Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody (AB125212)

Primary Antibodies

AB213363

Anti-CD68 antibody [EPR20545]

Multiplex immunohistochemistry - Anti-CD68 antibody [EPR20545] (AB213363)

組合せ製品

Assay Kits

AB287167

Human CD68 ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human CD68 ELISA Kit (AB287167)

Primary Antibodies

AB182422

Anti-CD163 antibody [EPR19518]

BOND RX™ Validated|20ul selling size|RabMAb|Advanced Validation|Recombinant

Flow Cytometry - Anti-CD163 antibody [EPR19518] (AB182422)

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

Primary Antibodies

AB64693

Anti-CD206 antibody

BOND RX™ Validated

Western blot - Anti-CD206 antibody (AB64693)

Primary Antibodies

AB178846

Anti-Iba1 antibody [EPR16588] - Microglia marker

20ul selling size|BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody [EPR16588] - Microglia marker (AB178846)

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Rat": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/50 - 1/100", "FlowCytIntra-species-notes": "Use 10μl of the suggested working dilution to label 106 cells in 100μl. <a href='/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody." } } }

style

left-column

製品の詳細

Product Specifications

Anti-CD68 antibody [ED1] (ab31630) is a mouse monoclonal antibody and is validated for use in Flow Cyt (Intra) in rat samples.
Anti-CD68 antibody [ED1] (ab31630) specifically detects CD68 (UniProt ID: P34810; Molecular weight: 35kDa) and is sold in 125 µg selling sizes.

Quality and Validation

Abcam's high quality validation processes ensure Anti-CD68 antibody [ED1] (ab31630) has high sensitivity and specificity.
Anti-CD68 antibody [ED1] (ab31630) has been cited over 403 times in peer reviewed journals and is trusted by the scientific community.
Anti-CD68 antibody [ED1] (ab31630) has 20 independent reviews from customers.

Target Information

CD68 is a transmembrane glycoprotein predominantly expressed on macrophages, playing a crucial role in phagocytosis and antigen presentation. It is involved in various pathological conditions, including neuroinflammatory diseases, where it helps clear cellular debris and modulate immune response. CD68 expression is associated with several diseases, particularly those involving macrophage activity, including, cancer, neuroinflammation, Granular cell tumor and histiocytic sarcoma.

This product should be stored undiluted.

style

left-column

出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

精製に関する特記事項

Purified from tissue culture supernatant.

バッファー組成

pH: 7.4 Preservative: 0.09% Sodium azide Constituents: PBS

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

style

left-column

補足情報

This supplementary information is collated from multiple sources and compiled automatically.

CD68 is a glycoprotein that functions as a scavenger receptor. It plays an important role in the regulation of cellular debris and apoptotic cell clearance. Researchers often refer to it as KP1 or macrosialin in the literature. CD68 with a molecular weight of approximately 110 kDa is highly expressed in macrophages and monocytes. The mucin-like structure of CD68 allows it to be heavily glycosylated which aids in its function. Researchers detect CD68 in tissues through methods like immunohistochemistry (IHC) immunofluorescence and CD68 staining and it is widely used as a macrophage marker in research.

Biological function summary

CD68 facilitates functions associated with the innate immune response. It serves as an important part of macrophages which contribute to immune surveillance and tissue homeostasis. Though it primarily operates on its own in some contexts CD68 might assist in forming complexes with other receptors to modulate phagocytic activity. This target is significant in atherosclerotic plaque stability as macrophages engulf lipids and cell debris through processes facilitated by CD68.

Pathways

CD68 is involved in pathways connected to inflammation and phagocytosis. The CD68 protein works closely with other scavenger receptors like CD36 to mediate uptake of oxidized low-density lipoproteins (oxLDL) in the lipid metabolism pathway. Additionally CD68 engagement can influence the toll-like receptor (TLR) signaling pathways thereby linking innate immunity and inflammatory responses critical for host defense and disease progression.

CD68 is associated with atherosclerosis and multiple sclerosis. In atherosclerosis its role becomes evident as it accumulates in macrophages within the plaques making it a marker of disease severity. In multiple sclerosis CD68 expression in macrophages and microglia indicates active demyelination and inflammation. It also relates to proteins such as CD36 in atherosclerosis where both mediate the uptake of modified LDL contributing to foam cell formation in plaques.

style

left-column

style

left-column

製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

ターゲットの情報

See full target information Cd68

style

left-column

文献 (490)

Recent publications for all applications. Explore the full list and refine your search

Hypertension research : official journal of the Japanese Society of Hypertension 48:2218-2233 PubMed40490489

2025

Dark-phase melatonin administration does not reduce blood pressure but induces changes in parameters related to the control of the cardiovascular system in spontaneously hypertensive rats.

Applications

Unspecified application

Species

Unspecified reactive species

Hana Mauer Sutovska,Lubos Molcan,Peter Stefanik,Michal Zeman

Journal of biological engineering 19:53 PubMed40468345

2025

Material and biological characterization of 3D knitted bioresorbable poly (D,L-lactide) (PLA) and polycaprolactone (PCL) scaffolds for soft tissue regeneration: from fabrication to in vivo performance.

Applications

Unspecified application

Species

Unspecified reactive species

Mélanie Dhayer,Vivien Barral,Damien Cleret,Amélia Jordao,Anne-Sophie Drucbert,Nicolas Germain,Sophie Dropsit,Patrice Maboudou,Salim Dekiouk,Stéphanie Brun,Christine Campagne,Éric Devaux,Pierre Guerreschi,Aurélie Cayla,Philippe Marchetti

Chemical science 16:12421-12438 PubMed40469767

2025

Ultra-high resolution magnetic resonance microscopy of gadolinium gold nanoparticle-labeled cells in the rat brain.

Applications

Unspecified application

Species

Unspecified reactive species

Alena Kisel,Minrui Luo,Matthew D Bailey,Harman Ghuman,Matthew Rotz,Vinícius P Campos,Marcelo A C Vieira,T Kevin Hitchens,Thomas J Meade,Michel Modo

Cardiovascular diabetology 24:235 PubMed40450269

2025

Tenascin-C drives cardiovascular dysfunction in a mouse model of diabetic cardiomyopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Zsuzsanna Arnold,Christopher Dostal,Petra Lujza Szabó,Ibrahim Aykac,Ana Isabel Antunes Goncalves,Silva Laura Sousa,Simge Baydar,Heidi Budde,Barnabás Váradi,György L Nadasy,Mária Szekeres,Camilla Hage,Lars H Lund,Sarah Costantino,Dietmar Abraham,Karin Zins,Seth Hallström,Martin Bilban,Bence Ágg,Nazha Hamdani,Péter Ferdinandy,Francesco Paneni,Attila Kiss,Bruno K Podesser

Skeletal muscle 15:12 PubMed40329365

2025

Forskolin treatment enhances muscle regeneration and shows therapeutic potential with limitations in Duchenne muscular dystrophy.

Applications

Unspecified application

Species

Unspecified reactive species

Andreea Iuliana Cojocaru,Kaouthar Kefi,Jean-Daniel Masson,Laurent Tiret,Frederic Relaix,Valentina Taglietti

Journal of molecular histology 56:106 PubMed40080193

2025

Exogenous mitochondria added on benefits for cellular prion protein overexpression in adipose-derived mesenchymal stem cells treatment on intracranial hemorrhage rat.

Applications

Unspecified application

Species

Unspecified reactive species

Kun-Chen Lin,Jui-Ning Yeh,Pei-Hsun Sung,Tsung-Cheng Yin,John Y Chiang,Chi-Ruei Huang,Yi-Ling Chen,Yi-Ting Wang,Kuan-Hung Chen,Hon-Kan Yip

International journal of nanomedicine 20:2761-2778 PubMed40061881

2025

Quercetin-Loaded Nanoparticle-Modified Decellularized Tissue-Engineered Vascular Graft Regulates Macrophage Polarization and Promotes In Vivo Graft Remodeling.

Applications

Unspecified application

Species

Unspecified reactive species

Tun Wang,Zhenyu He,Peng Lu,Sheng Liao,Siyuan Cheng,Tianjian Wang,Yangyang An,Zibo Cheng,Chang Shu

Nutrients 17: PubMed40005027

2025

The Effects of White Wine and Ethanol Consumption on the Proliferative Phase of Repair After a Surgically Induced Myocardial Infarction in Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Danica Boban,Ivica Grković,Ana Marija Dželalija,Diana Gujinović,Ivana Mudnić,Mladen Boban

American journal of physiology. Heart and circulatory physiology 328:H711-H718 PubMed39982452

2025

Inhibition of kinin B1 receptor alleviates SARS-CoV-2-induced long-lasting cardiovascular complications.

Applications

Unspecified application

Species

Unspecified reactive species

Drew Theobald,Lisandra E de Castro Braz,Shaw M Akula,Jeffrey B Eells,Srinivas Sriramula

Nature communications 16:1674 PubMed39955276

2025

Janus decellularized membrane with anisotropic cell guidance and anti-adhesion silk-based coatings for spinal dural repair.

Applications

Unspecified application

Species

Unspecified reactive species

Xuewei Bi,Zhinan Mao,Linhao Li,Yilin Zhang,Lingbing Yang,Sen Hou,Juan Guan,Yufeng Zheng,Xiaoming Li,Yubo Fan

View all publications

style

left-column

style

left-column

style

right-column

Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com