Anti-CD64 抗体 [EPR23840-139] - BSA and Azide free (ab302902)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD64 antibody [EPR23840-139] (BSA and Azide free) (AB302902)](https://www.abcam.co.jp/ps/products/302/ab302902/Images/AB302902-2-anti-cd64-antibody-epr23840-139-immunohistochemistry-human-liver.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD64 antibody [EPR23840-139] (BSA and Azide free) (AB302902)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23840-139] to CD64 - BSA and Azide free
- Suitable for: IHC-P
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-CD64 antibody [EPR23840-139] - BSA and Azide free
CD64 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR23840-139] to CD64 - BSA and Azide free -
由来種
Rabbit -
アプリケーション
適用あり: IHC-Pmore details
適用なし: Flow Cyt,ICC/IF,IP or WB -
種交差性
交差種: Human
非交差種: Mouse, Rat -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- IHC-P: Human liver, spleen and cerebrum tissue. HEK-293T cells transfected with a FCGR1A (CD64) expression vector containing a his tag.
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特記事項
AB302902 is a carrier free version of AB302901.
AB302901 does not react in: WB with human, mouse, and rat samples; IHC-P with mouse and rat tissues; ICC/IF, Flow Cytometry and IP with human tissues.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 7.2
Constituent: 100% PBS -
キャリア・フリー
はい -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR23840-139 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab302902の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P | (1) |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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| 特記事項 |
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IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ターゲット情報
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機能
High affinity receptor for the Fc region of immunoglobulins gamma. Functions in both innate and adaptive immune responses. -
組織特異性
Monocyte/macrophage specific. -
配列類似性
Belongs to the immunoglobulin superfamily. FCGR1 family.
Contains 3 Ig-like C2-type (immunoglobulin-like) domains. -
翻訳後修飾
Phosphorylated on serine residues. -
細胞内局在
Cell membrane. Stabilized at the cell membrane through interaction with FCER1G. - Information by UniProt
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参照データベース
- Entrez Gene: 2209 Human
- Entrez Gene: 2210 Human
- Omim: 146760 Human
- Omim: 601502 Human
- Omim: 601503 Human
- SwissProt: A6NKC4 Human
- SwissProt: P12314 Human
- SwissProt: Q92637 Human
see all -
別名
- CD 64 antibody
- CD64 antibody
- CD64 antigen antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD64 antibody [EPR23840-139] (BSA and Azide free) (AB302902)
This data was developed using AB302901, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human liver tissue labeling CD64 with ab302901 at 1/500 dilution (0.958 µg/ml) followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). Positive staining in Kupffer cells of human liver is observed (PMID: 26512139). The section was incubated with ab302901 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: A ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD64 antibody [EPR23840-139] (BSA and Azide free) (AB302902)](https://www.abcam.co.jp/ps/products/302/ab302902/Images/AB302902-3-anti-cd64-antibody-epr23840-139-immunohistochemistry-human-spleen.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD64 antibody [EPR23840-139] (BSA and Azide free) (AB302902)This data was developed using AB302901, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human spleen tissue labeling CD64 with ab302901 at 1/500 dilution (0.958 µg/ml) followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). Positive staining on human spleen is observed (PMID: 26512139). The section was incubated with ab302901 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Aready to use LeicaDS9800 (BOND™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD64 antibody [EPR23840-139] (BSA and Azide free) (AB302902)](https://www.abcam.co.jp/ps/products/302/ab302902/Images/AB302902-4-anti-cd64-antibody-epr23840-139-immunohistochemistry-human-cerebrum.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD64 antibody [EPR23840-139] (BSA and Azide free) (AB302902)This data was developed using ab302901, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling CD64 with ab302901 at 1/500 dilution (0.958 µg/ml) followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). Positive staining on microglia of human cerebrum is observed (PMID: 23452918). The section was incubated with ab302901 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: A ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD64 antibody [EPR23840-139] (BSA and Azide free) (AB302902)](https://www.abcam.co.jp/ps/products/302/ab302902/Images/AB302902-5-anti-cd64-antibody-epr23840-139-immunohistochemistry-a-hek-293t-cells-t.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD64 antibody [EPR23840-139] (BSA and Azide free) (AB302902)This data was developed using AB302901, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded transfected HEK-293T cells labeling CD64 with ab302901 at 1/5000 dilution (0.0958 µg/ml) followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). Positive staining is observed on (Panel A) HEK-293T cells transfected with a FCGR1A (CD64) expression vector containing a his tag. No staining on (Panel B) HEK-293T cells transfected with a FCGR1B expression vector containing a his tag and (Panel C) HEK-293T cells transfected with empty vector containing a his tag. The sections ware incubated with ab302901 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: A ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
-
![Anti-CD64 antibody [EPR23840-139] (BSA and Azide free) (AB302902)](https://www.abcam.co.jp/ps/products/302/ab302902/Images/AB302902-1-Benefits-of-Recombinant-antibodies.jpg)
Anti-CD64 antibody [EPR23840-139] (BSA and Azide free) (AB302902)
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
Certificate of Compliance
参考文献 (0)
ab302902 は論文での使用が確認できていません。
