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AB227892

Anti-CD63 抗体 [EPR21151] - BSA and Azide free

Anti-CD63 antibody [EPR21151] - BSA and Azide free

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(3 Publications)

Rabbit Recombinant Monoclonal CD63 antibody. Carrier free. Suitable for WB, ICC/IF and reacts with Mouse samples. Cited in 3 publications.

別名を表示する

CD63, CD63 antigen, Cd63

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-CD63 antibody [EPR21151] - BSA and Azide free (AB227892)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CD63 antibody [EPR21151] - BSA and Azide free (AB227892)

Immunofluorescent analysis of 100% methanol-fixed bEND.3 (mouse brain endothelioma cell line) cells labeling CD63 with ab217345 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining in bEND.3 cell line.

The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab217345).

Immunocytochemistry/ Immunofluorescence - Anti-CD63 antibody [EPR21151] - BSA and Azide free (AB227892)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CD63 antibody [EPR21151] - BSA and Azide free (AB227892)

Immunofluorescent analysis of 100% methanol-fixed NIH/3T3 (mouse embryo fibroblast cell line) cells labeling CD63 with ab217345 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining in the NIH/3T3 cell line.

The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab217345).

関連する標識済み抗体及び組成の異なる製品 (7)

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR21151

アイソタイプ

IgG

キャリアフリー

Yes

交差種

Mouse

アプリケーション

WB, ICC/IF

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
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製品の詳細

ab227892 is the carrier-free version of ab217345.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.2 - 7.4 Constituents: PBS
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
+4°C
保管に関する情報
Do Not Freeze
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

CD63 is a protein commonly known as LAMP-3 a member of the tetraspanin family with a molecular weight of approximately 25-65 kDa depending on post-translational modifications such as glycosylation. It resides mainly on the membrane of intracellular vesicles such as lysosomes and platelets. CD63 also appears on the surface of activated cells particularly in immune cells. Researchers often use CD63 as a marker in assays including CD63 western blot techniques due to its distinct expression patterns.
Biological function summary

CD63 participates in various cellular processes including membrane trafficking cell adhesion and signal transduction. It associates with integrins and other tetraspanins forming complexes that influence cellular adhesion and migration. Through its role in exosome formation CD63 contributes significantly to intercellular communication by facilitating the transfer of proteins lipids and RNA between cells.

Pathways

CD63 plays a role in pathways linked to cell proliferation and migration and immune response regulation. Within the integrin-mediated signaling pathway CD63 interacts with proteins such as integrins to promote cell adhesion and migration. CD63 also links to vesicle trafficking pathways collaborating with proteins involved in exocytosis and endocytosis which affect cell membrane restructuring and intracellular transport.

CD63 shows connections with cancer and immune system disorders. Its role in promoting cell adhesion and migration links it with cancer metastasis including melanoma and breast cancer where CD63 expression levels often rise. Additionally CD63 associates with allergic reactions due to its involvement in histamine release where it impacts immune proteins like FcεRI which participate in allergy pathogenesis.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Functions as a cell surface receptor for TIMP1 and plays a role in the activation of cellular signaling cascades. Plays a role in the activation of ITGB1 and integrin signaling, leading to the activation of AKT, FAK/PTK2 and MAP kinases. Promotes cell survival, reorganization of the actin cytoskeleton, cell adhesion, spreading and migration, via its role in the activation of AKT and FAK/PTK2. Plays a role in VEGFA signaling via its role in regulating the internalization of KDR/VEGFR2. Plays a role in intracellular vesicular transport processes, and is required for normal trafficking of the PMEL luminal domain that is essential for the development and maturation of melanocytes. Plays a role in the adhesion of leukocytes onto endothelial cells via its role in the regulation of SELP trafficking. May play a role in mast cell degranulation in response to Ms4a2/FceRI stimulation, but not in mast cell degranulation in response to other stimuli.
See full target information CD63 antigen
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文献 (3)

Recent publications for all applications. Explore the full list and refine your search

Precision clinical medicine 7:pbae028 PubMed39540022

2024

Targeted nuclear degranulation of neutrophils promotes the progression of pneumonia in ulcerative colitis.

Applications

Unspecified application

Species

Unspecified reactive species

Yiming Shao,Qibing Zheng,Xiaobei Zhang,Ping Li,Xingxin Gao,Liming Zhang,Jiahong Xu,Lingchao Meng,Yanyun Tian,Qinqin Zhang,Guangxi Zhou

Gerontology 70:1294-1304 PubMed39413738

2024

LncRNA MALAT1 Facilitates Parkinson's Disease Progression by Increasing SOCS3 Promoter Methylation.

Applications

Unspecified application

Species

Unspecified reactive species

Yuqi Liu,Dan Feng,Fenfen Liu,Yun Liu,Fangya Zuo,Yujie Wang,Lanlan Chen,Xiuhong Guo,Jinyong Tian

Breast cancer (Dove Medical Press) 15:403-419 PubMed37274058

2023

Rab1A-Mediated Exosomal Sorting of miR-200c Enhances Breast Cancer Lung Metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Yuting Liu,Jie Tang,Xiaolan Qiu,Lucy A Teng,Mukesh K Sriwastva,Xuedong Han,Zhi Li,Minmin Liu,Shuangyue Liu,Dongzhu Da,Zhi Li,Linlin Zhen,Yi Ren
View all publications
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代替製品

Primary Antibodies

AB215431

PE Anti-CD63 antibody [NKI/C3] - Late Endosome Marker

Lab Essentials

Flow Cytometry - PE Anti-CD63 antibody [NKI/C3] - Late Endosome Marker (AB215431)

  • 0
  • 0
Primary Antibodies

AB215891

Anti-CD63 antibody [LAMP3/968]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD63 antibody [LAMP3/968] (AB215891)

  • 0
  • 0
Primary Antibodies

AB213090

Anti-CD63 antibody [NKI/C3] - BSA and Azide free

Flow Cytometry - Anti-CD63 antibody [NKI/C3] - BSA and Azide free (AB213090)

  • 1
  • 1
Primary Antibodies

AB205540

PE Anti-CD63 antibody [MX-49.129.5]

Flow Cytometry - PE Anti-CD63 antibody [MX-49.129.5] (AB205540)

  • 0
  • 1
Primary Antibodies

AB18236

PE Anti-CD63 antibody [MEM-259]

Flow Cytometry - PE Anti-CD63 antibody [MEM-259] (AB18236)

  • 0
  • 0
Primary Antibodies

AB234251

PE/Cy5® Anti-CD63 antibody [MEM-259]

Flow Cytometry - PE/Cy5® Anti-CD63 antibody [MEM-259] (AB234251)

  • 0
  • 0
Primary Antibodies

AB77227

PerCP Anti-CD63 antibody [MEM-259]

Flow Cytometry - PerCP Anti-CD63 antibody [MEM-259] (AB77227)

  • 4
  • 2
Primary Antibodies

AB134331

Biotin Anti-CD63 antibody [MEM-259]

Flow Cytometry - Biotin Anti-CD63 antibody [MEM-259] (AB134331)

  • 3
  • 1
Primary Antibodies

AB108950

Anti-CD63 antibody [AD1]

Flow Cytometry - Anti-CD63 antibody [AD1] (AB108950)

  • 0
  • 0
Primary Antibodies

AB216130

Anti-CD63 antibody - Late Endosome Marker

Lab Essentials

Western blot - Anti-CD63 antibody - Late Endosome Marker (AB216130)

  • 0
  • 0
Primary Antibodies

AB1318

Anti-CD63 antibody [NK1/C3]

KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD63 antibody [NK1/C3] (AB1318)

  • 4
  • 1
Primary Antibodies

AB68418

Anti-CD63 antibody

KO Validated

Western blot - Anti-CD63 antibody (AB68418)

  • 5
  • 1
Primary Antibodies

AB193349

Anti-CD63 antibody [MX-49.129.5]

BOND RX™ Validated|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD63 antibody [MX-49.129.5] (AB193349)

  • 0
  • 2
Primary Antibodies

AB134045

Anti-CD63 antibody [EPR5702] - Late Endosome Marker

RabMAb|Recombinant|KO Validated|Lab Essentials|Trial Size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD63 antibody [EPR5702] - Late Endosome Marker (AB134045)

  • 3
  • 6
Primary Antibodies

AB215821

Anti-CD63 antibody [EPR5702] - BSA and Azide free

RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD63 antibody [EPR5702] - BSA and Azide free (AB215821)

  • 0
  • 0

組合せ製品

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AB125011

Anti-TSG101 antibody [EPR7130(B)]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry - Anti-TSG101 antibody [EPR7130(B)] (AB125011)

  • 4
  • 10
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB22595

Anti-Calnexin antibody - ER Marker

Lab Essentials|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-Calnexin antibody - ER Marker (AB22595)

  • 5
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Primary Antibodies

AB9485

Anti-GAPDH antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (AB9485)

  • 5
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