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Anti-CD62P 抗体 [Psel.KO.2.5] (ab118522)

Submit a review Q&A (4)References (2)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD62P antibody [Psel.KO.2.5] (ab118522)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD62P antibody [Psel.KO.2.5] (ab118522)
  • Flow Cytometry - Anti-CD62P antibody [Psel.KO.2.5] (ab118522)

Key features and details

  • Mouse monoclonal [Psel.KO.2.5] to CD62P
  • Suitable for: IHC-P, Flow Cyt
  • Reacts with: Human
  • Isotype: IgG1

製品の概要

  • 製品名

    Anti-CD62P antibody [Psel.KO.2.5]
    CD62P 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [Psel.KO.2.5] to CD62P

  • 由来種

    Mouse

  • アプリケーション

    適用あり: IHC-P, Flow Cytmore details

  • 種交差性

    交差種: Human

  • 免疫原

    Tissue, cells or virus corresponding to Human CD62P. CD62P transfected 300.19 cells

  • ポジティブ･コントロール

    • Human Spleen and Tonsil tissues; Thrombin activated Human platelets.

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab118522の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-P
Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt
1/1 - 1/50.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
特記事項
IHC-P
Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt
1/1 - 1/50.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能

    Ca(2+)-dependent receptor for myeloid cells that binds to carbohydrates on neutrophils and monocytes. Mediates the interaction of activated endothelial cells or platelets with leukocytes. The ligand recognized is sialyl-Lewis X. Mediates rapid rolling of leukocyte rolling over vascular surfaces during the initial steps in inflammation through interaction with PSGL1.

  • 組織特異性

    Stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. Upon cell activation by agonists, P-selectin is transported rapidly to the cell surface.

  • 関連疾患

    Defects in SELP may be a cause of susceptibility to ischemic stroke (ISCHSTR) [MIM:601367]; also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors.

  • 配列類似性

    Belongs to the selectin/LECAM family.
    Contains 1 C-type lectin domain.
    Contains 1 EGF-like domain.
    Contains 9 Sushi (CCP/SCR) domains.

  • 細胞内局在

    Membrane.
  • Information by UniProt

  • 参照データベース

    • 別名

      • Antigen CD62 antibody
      • CD62 antibody
      • CD62 antigen-like family member P antibody
      • CD62P antibody
      • GMP 140 antibody
      • GMP-140 antibody
      • GMP140 antibody
      • GMRP antibody
      • Granule membrane protein 140 antibody
      • Granulocyte membrane protein antibody
      • GRMP antibody
      • LECAM 3 antibody
      • LECAM3 antibody
      • Leukocyte endothelial cell adhesion molecule 3 antibody
      • Leukocyte-endothelial cell adhesion molecule 3 antibody
      • LYAM3_HUMAN antibody
      • P Selectin antibody
      • P-selectin antibody
      • PADGEM antibody
      • Platelet activation dependent granule-external membrane protein antibody
      • Platelet alpha granule membrane protein antibody
      • PSEL antibody
      • Selectin P (granule membrane protein 140kDa, antigen CD62) antibody
      • SELP antibody
      see all

    画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD62P antibody [Psel.KO.2.5] (ab118522)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD62P antibody [Psel.KO.2.5] (ab118522)
      Immunohistochemical analysis of CD62P expression in Formalin-Fixed, Paraffin-Embedded Human Spleen using 10 µg/ml ab118522 followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD62P antibody [Psel.KO.2.5] (ab118522)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD62P antibody [Psel.KO.2.5] (ab118522)
      Immunohistochemical analysis of CD62P expression in Formalin-Fixed, Paraffin-Embedded Human Tonsil using 10 µg/ml ab118522 followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.
    • Flow Cytometry - Anti-CD62P antibody [Psel.KO.2.5] (ab118522)
      Flow Cytometry - Anti-CD62P antibody [Psel.KO.2.5] (ab118522)
      ab118522 at 10µl/106 cells in 100µl staining CD62P in Thrombin activated Human platelets by Flow cytometry.

    参考文献 (2)

    ab118522 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab118522 は 2 報の論文で使用されています。

    • Preidl RHM  et al. Endothelial inflammatory and thrombogenic expression changes in microvascular anastomoses - An immunohistochemical analysis. J Craniomaxillofac Surg 49:422-429 (2021). PubMed: 33608202
    • Preidl RHM  et al. Long-term endothelial dysfunction in irradiated vessels: an immunohistochemical analysis. Strahlenther Onkol 195:52-61 (2019). PubMed: 30324290

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-4 of 4 Abreviews or Q&A

    Question

    Our customer has answered your questions below in blue. Also they tried the antigen retrieval steps and did not get any improvement.

    Could you advise on how to proceed?

    Best regards,


    I have read the details customer have kindly provided. However I have few other questions;

    - Does both GR62917-2 AND GR62917-3 lot numbers were negative?Yes, both lot numbers were negative, no staining at all.
    - What is the species of secondary antibody?As secondary antibody we use Envision reagent from DAKO.The EnVision™ reagent of this kit is a peroxidase-conjugated polymer backbone, which, in addition, also carries secondary antibody molecules directed against rabbit and mouse immunoglobulins. The combination of several peroxidase molecules and several secondary antibody molecules on the same polymer provides a simple, yet sensitive, visualization system.
    - Does negative mean no staining at all or high background? We see no staining at all.
    - Have you tried optimizing of antigen retrieval step; the optimization is necessary when antibodies gives no staining or high background? No, weonly did antigen retrieval for 15 minutes in Citrate buffer inmicrowave.
    - Have you tried incubating the sections overnight? No, we did not.
    - For how long the tissues were fixed in formalin? The time of fixation in formalin was about 4 hours.

    Recommendations for further optimizations

    -Try antigen retrieval for 5,10,15,20 minutes by incubating the section in microwave (700 watt) with citrate buffer pH6.0. The time range will help getting the optimized retrieval time.
    - Check the pH of buffer, antibodies don't work if the buffer pH is high or low.
    - Incubated the primary overnight at 4C.
    - The tissue should not be fixed for more than 24 hours.
    - Add 0.2% Tween in the PBS for antibody dilution, blocking solution and washing solution.
    - Block the sections with 10% normal serum or 5% BSA for 1-2 hours.

    I hope these troubleshooting suggestions will help to get good staining of target protein. Please do not hesitate to contact me if the results do not improve.




    .

    Read More
    Answer

    Thank you for your response.
    Could you please confirm the order details please.

    Date of purchase,

    - Abcam Order Number or

    - Your PON

    Question: Has the customer used the same detection system (peroxidase-conjugated polymer from EnVision™) successfully with a different primary antibody?

    I look forward to hearing from you soon.

    Read More

    Question

    We have forwarded your questions to our client and they have answered as follows:


    I have read the details customer have kindly provided. However I have few other questions;

    - Does both GR62917-2 AND GR62917-3 lot numbers were negative?Yes, both lot numbers were negative, no staining at all.
    - What is the species of secondary antibody?As secondary antibody we use Envision reagent from DAKO.The EnVision™ reagent of this kit is a peroxidase-conjugated polymer backbone, which, in addition, also carries secondary antibody molecules directed against rabbit and mouse immunoglobulins. The combination of several peroxidase molecules and several secondary antibody molecules on the same polymer provides a simple, yet sensitive, visualization system.
    - Does negative mean no staining at all or high background? We see no staining at all.
    - Have you tried optimizing of antigen retrieval step; the optimization is necessary when antibodies gives no staining or high background? No, weonly did antigen retrieval for 15 minutes in Citrate buffer inmicrowave.
    - Have you tried incubating the sections overnight? No, we did not.
    - For how long the tissues were fixed in formalin? The time of fixation in formalin was about 4 hours.

    Recommendations for further optimizations

    -Try antigen retrieval for 5,10,15,20 minutes by incubating the section in microwave (700 watt) with citrate buffer pH6.0. The time range will help getting the optimized retrieval time.
    - Check the pH of buffer, antibodies don't work if the buffer pH is high or low.
    - Incubated the primary overnight at 4C.
    - The tissue should not be fixed for more than 24 hours.
    - Add 0.2% Tween in the PBS for antibody dilution, blocking solution and washing solution.
    - Block the sections with 10% normal serum or 5% BSA for 1-2 hours.

    I hope these troubleshooting suggestions will help to get good staining of target protein. Please do not hesitate to contact me if the results do not improve.

    They are currently looking into optimizing their antigen retrieval steps and the other tips you have kindly provided.

    We look forward to your response.

    Best regards,

    Read More
    Answer

    Thank you for your email. Please let me know how the staining go after following the troubleshooting suggestions.

    Read More

    Question

    Thank you for the quick response.

    Our customer has filled in the technical questionnaire and we have attached it to this mail.

    We look forward to hearing your opinion about the problem encountered.

    Best regards,

    Read More
    Answer

    Thank you for your email.

    I have read the details customer have kindly provided. However I have few other questions;

    - Does both GR62917-2 AND GR62917-3 lot numbers were negative?
    - What is the species of secondary antibody?
    - Does negative mean no staining at all or high background?
    - Have you tried optimizing of antigen retrieval step; the optimization is necessary when antibodies gives no staining or high background?
    - Have you tried incubating the sections overnight?
    - For how long the tissues were fixed in formalin?

    Recommendations for further optimizations

    -Try antigen retrieval for 5,10,15,20 minutes by incubating the section in microwave (700 watt) with citrate buffer pH6.0. The time range will help getting the optimized retrieval time.
    - Check the pH of buffer, antibodies don't work if the buffer pH is high or low.
    - Incubated the primary overnight at 4C.
    - The tissue should not be fixed for more than 24 hours.
    - Add 0.2% Tween in the PBS for antibody dilution, blocking solution and washing solution.
    - Block the sections with 10% normal serum or 5% BSA for 1-2 hours.

    I hope these troubleshooting suggestions will help to get good staining of target protein. Please do not hesitate to contact me if the results do not improve.

    Read More

    Question

    One of our customers is experiencing problems with antibody AB118522 in IHC-P. Could you send us a technical questionnaire for this antibody, to complete this request?

    Best regards,

    Read More
    Answer

    Thank you for contacting us.

    I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

    I am attaching our questionnaire so that we can gather further information regarding the samples tested and the protocol used. Once we receive the completed questionnaire, we will look at the protocol and see if there are any suggestions we can make that may improve the results. This information will also allow us to investigate this case internally and initiate additional testing where necessary. If the product was purchased in the last six months and is being used according to our Abpromise, we would be happy to replace or refund the antibody.

    I look forward to receiving your reply.

    Read More

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