Anti-CD62L 抗体 [MEL-14] - BSA and Azide free (ab256147)
![Immunocytochemistry/ Immunofluorescence - Anti-CD62L antibody [MEL-14] - BSA and Azide free (ab256147)](https://www.abcam.co.jp/ps/products/256/ab256147/Images/ab256147-484078-anticd62lantibodymel14immunocytochemistrymouse.jpg "Immunocytochemistry/ Immunofluorescence - Anti-CD62L antibody [MEL-14] - BSA and Azide free (ab256147)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rat monoclonal [MEL-14] to CD62L - BSA and Azide free
- Suitable for: Flow Cyt, ICC/IF
- Reacts with: Mouse
Related conjugates and formulations
製品の概要
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製品名
Anti-CD62L antibody [MEL-14] - BSA and Azide free
CD62L 一次抗体 製品一覧 -
製品の詳細
Rat monoclonal [MEL-14] to CD62L - BSA and Azide free -
由来種
Rat -
特異性
ab119834 reacts with mouse CD62L, a 75 kDa single chain type I glycoprotein expressed on most peripheral blood B lymphocytes, T lymphocytes, monocytes and granulocytes; it is also present on a subset of NK cells and certain hematopoietic malignant cells.
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アプリケーション
適用あり: Flow Cyt, ICC/IFmore details
適用なし: IHC-P -
種交差性
交差種: Mouse -
免疫原
Tissue, cells or virus. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- ICC/IF: Mouse spleen cells. Flow Cyt: Mouse splenocytes.
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特記事項
ab256147 is the carrier free version of ab119834.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
バッファー
pH: 7.4
Constituent: 100% PBS -
キャリア・フリー
はい -
Concentration information loading... -
精製度
Ion Exchange Chromatography -
ポリ/モノ
モノクローナル -
クローン名
MEL-14 -
アイソタイプ
IgG2a -
研究分野
関連製品
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Alternative Versions
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab256147の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Flow Cyt |
Use at an assay dependent concentration.
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| ICC/IF |
Use at an assay dependent concentration.
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| 特記事項 |
|---|
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Flow Cyt
Use at an assay dependent concentration. |
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ICC/IF
Use at an assay dependent concentration. |
ターゲット情報
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機能
Cell surface adhesion protein. Mediates the adherence of lymphocytes to endothelial cells of high endothelial venules in peripheral lymph nodes. Promotes initial tethering and rolling of leukocytes in endothelia. -
組織特異性
Expressed in B cell lines and T lymphocytes. -
配列類似性
Belongs to the selectin/LECAM family.
Contains 1 C-type lectin domain.
Contains 1 EGF-like domain.
Contains 2 Sushi (CCP/SCR) domains. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 20343 Mouse
- SwissProt: P18337 Mouse
- Unigene: 1461 Mouse
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別名
- A.11 antibody
- AI528707 antibody
- CD62 antigen ligand antibody
see all
画像
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Immunocytochemistry/ Immunofluorescence - Anti-CD62L antibody [MEL-14] - BSA and Azide free (ab256147)
This data was developed using ab119834, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Mouse spleen cells labelling CD62L with ab119834 at 1/100 dilution, followed by ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing membranous staining in some subset of mouse spleen cells is observed. ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution, followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) at 1/1000 dilution (Red). The Nuclear counterstain was DAPI (Blue).
-ve control 1: ab119834 at 1/100 dilution, followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) at 1/1000 dilution.
-ve control 2: ab179513 at 1/200 dilution, followed by ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
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![Flow Cytometry - Anti-CD62L antibody [MEL-14] - BSA and Azide free (ab256147)](https://www.abcam.co.jp/ps/products/256/ab256147/Images/ab256147-484080-anticd62lantibodymel14flowcytometrymousesplenocytes.jpg)
Flow Cytometry - Anti-CD62L antibody [MEL-14] - BSA and Azide free (ab256147)This data was developed using ab119834, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of Mouse splenocyte cells labelling CD62L with ab119834 at 1/500 dilution (0.2 ug). Goat anti rat IgG (Alexa Fluor® 488, ab150157) at 1/2000 dilution was used as the secondary antibody. Cells were stained with rat IgG (Left) or ab119834 (Right). Then stained with anti-CD4 conjugated to Alexa Fluor®647 and anti-CD3 conjugated to PerCP.
Gated on viable CD3+ T cells. -
![Flow Cytometry - Anti-CD62L antibody [MEL-14] - BSA and Azide free (ab256147)](https://www.abcam.co.jp/ps/products/256/ab256147/Images/ab256147-484079-anticd62lantibodymel14flowcytometrymouse-2.jpg)
Flow Cytometry - Anti-CD62L antibody [MEL-14] - BSA and Azide free (ab256147)This data was developed using ab119834, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of Mouse splenocyte cells labelling CD62L with ab119834 at 1/500 dilution (0.2 ug). Goat anti rat IgG (Alexa Fluor® 488, ab150157) at 1/2000 dilution was used as the secondary antibody. Cells were stained with rat IgG (Left) or ab119834 (Right). Then stained with anti-CD4 conjugated to Alexa Fluor®647. Gated on viable lymphocytes.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (0)
ab256147 は論文での使用が確認できていません。
