Anti-CD62E 抗体 [EPR25616-62] (ab300557)
![Western blot - Anti-CD62E antibody [EPR25616-62] (ab300557)](https://www.abcam.co.jp/ps/products/300/ab300557/Images/AB300557-487445-anti-cd62e-antibody-epr2561662-western-blot-humancd62e-active-protein-10-ng.jpg "Western blot - Anti-CD62E antibody [EPR25616-62] (ab300557)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR25616-62] to CD62E
- Suitable for: ICC/IF, WB, Flow Cyt
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-CD62E antibody [EPR25616-62]
CD62E 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR25616-62] to CD62E -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, WB, Flow Cytmore details
適用なし: IHC-Fr,IHC-P or IP -
種交差性
交差種: Human
非交差種: Mouse, Rat -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- WB: HUVEC treated with 20 ng/ml IL-1 beta, human CD62E active protein. ICC: HUVEC (human umbilical vein endothelial cell). Flow cyt.: HUVEC (human umbilical vein endothelial cell) treated with 20 ng/ml IL-1 beta.
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特記事項
ab300557 does not react in IHC with human, mouse and rat. It does not react in IHC-Fr with mouse and rat nor in IP with human.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR25616-62 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab300557の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF |
1/50.
|
|
| WB |
1/1000.
|
|
| Flow Cyt |
1/500.
|
| 特記事項 |
|---|
|
ICC/IF
1/50. |
|
WB
1/1000. |
|
Flow Cyt
1/500. |
ターゲット情報
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機能
Cell-surface glycoprotein having a role in immunoadhesion. Mediates in the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with PSGL1/SELPLG. May have a role in capillary morphogenesis. -
配列類似性
Belongs to the selectin/LECAM family.
Contains 1 C-type lectin domain.
Contains 1 EGF-like domain.
Contains 6 Sushi (CCP/SCR) domains. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 6401 Human
- Omim: 131210 Human
- SwissProt: P16581 Human
- Unigene: 82848 Human
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別名
- CD26E antibody
- CD62 antigen-like family member E antibody
- CD62E antibody
see all
画像
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Western blot - Anti-CD62E antibody [EPR25616-62] (ab300557)Anti-CD62E antibody [EPR25616-62] (ab300557) at 1/1000 dilution + Human CD62E active protein, 10 ng
Secondary
Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Developed using the ECL technique.
Observed band size: 115 kDa why is the actual band size different from the predicted?
Exposure time: 180 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST
This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.
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![Western blot - Anti-CD62E antibody [EPR25616-62] (ab300557)](https://www.abcam.co.jp/ps/products/300/ab300557/Images/AB300557-487756-anti-cd62e-rabint-monoclonal-antibody-epr2561662-western-blot.jpg)
Western blot - Anti-CD62E antibody [EPR25616-62] (ab300557)All lanes : PCI 34051, HDAC8 inhibitor (ab300227) at 1/1000 dilution
Lane 1 : Untreated HUVEC (human umbilical vein endothelial cell), whole cell lysat
Lane 2 : HUVEC treated with 20ng/ml IL-lbeta for 4h, whole cell lysate
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Developed using the ECL technique.
Observed band size: 115 kDa why is the actual band size different from the predicted?
Exposure time: 81 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST
This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.
The molecular weight observed is consistent with what has been described in the literature (PMID: 16715142; PMID: 7589110).
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![Immunocytochemistry/ Immunofluorescence - Anti-CD62E antibody [EPR25616-62] (ab300557)](https://www.abcam.co.jp/ps/products/300/ab300557/Images/AB300557-3-anti-cd62e-antibody-epr2561662-immunocytochemistry-huvec.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CD62E antibody [EPR25616-62] (ab300557)This data was developed using ab300557, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HUVEC (human umbilical vein endothelial cells) labeling CD62E with ab300557 at 1/50 dilution (9.36 μg/ml), followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (2 μg/ml) (Green). Confocal image showing increased cytoplasmic and membrane staining in HUVEC cells treated with interleukin 1-lbeta (20 ng/ml) for 4 h (PMID: 16715142).
ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilutioin (2.5 μg/mL) (Red). The nuclear counterstain was DAPI (Blue).
Secondary antibody only control: PBS was used instead of primary antibody, followed by a preadsorbed secondary antibody ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 μg/ml). HUVEC cells treated and untreated with interleukin 1-beta (20 ng/ml) for 4 h were tested.
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![Flow Cytometry - Anti-CD62E antibody [EPR25616-62] (AB300557)](https://www.abcam.co.jp/ps/products/300/ab300557/Images/AB300557-2-anti-cd62e-antibody-epr2561662-flow-cytometry-huvec.jpg)
Flow Cytometry - Anti-CD62E antibody [EPR25616-62] (AB300557)Flow cytometric analysis of HUVEC (human umbilical vein endothelial cell) treated with 20 ng/ml interleukin 1 beta for 4 hours (Right) / Untreated control (Left) cells labelling CD62E with ab300557 at 1/500 dilution (0.1 μg). Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody. Gated on viable cells.
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![Anti-CD62E antibody [EPR25616-62] (AB300557)](https://www.abcam.co.jp/ps/products/300/ab300557/Images/AB300557-1-Benefits-of-Recombinant-antibodies.jpg)
Anti-CD62E antibody [EPR25616-62] (AB300557)
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (1)
ab300557 は 1 報の論文で使用されています。
- Wang X et al. Vascular Protective Effects of Malus toringoides (Rehd.) Hughes Extracts and their Mechanism in Diabetic Rats and HUVECs. Evid Based Complement Alternat Med 2022:4348435 (2022). PubMed: 36310615