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AB9181

Anti-CD6/T12 抗体 [MEM-98]

Anti-CD6/T12 antibody [MEM-98]

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(2 Publications)

Mouse Monoclonal CD6/T12 antibody. Suitable for Flow Cyt, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Native Full Length Protein corresponding to Human T-cell differentiation antigen CD6.

別名を表示する

CD6, T-cell differentiation antigen CD6, T12, TP120

3 Images
Flow Cytometry - Anti-CD6/T12 antibody [MEM-98] (AB9181)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD6/T12 antibody [MEM-98] (AB9181)

Flow cytometric analysis of human peripheral whole blood labeling CD6/T12 with ab9181 at 2µg/ml. Secondary used is a goat anti-mouse-APC.

Flow Cytometry - Anti-CD6/T12 antibody [MEM-98] (AB9181)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD6/T12 antibody [MEM-98] (AB9181)

Flow cytometric analysis of human CD6 positive lymphocytes (red-filled) / neutrophil granulocytes (black-dashed) from peripheral whole blood cells labeling CD6/T12 with ab9181 at 2µg/ml. Secondary used is a goat anti-mouse-APC.

Western blot - Anti-CD6/T12 antibody [MEM-98] (AB9181)
  • WB

Supplier Data

Western blot - Anti-CD6/T12 antibody [MEM-98] (AB9181)

Nitrocellulose membrane blot was probed with ab9181 used at 1 µg/ml, followed by IRDye 800CW Goat-anti-Mouse IgG (green). Mouse anti-GAPDH monoclonal antibody conjugated with DyLight 680 (0.1 µg/ml), was used as the loading control (red). Multiplex fluorescent Western blot detection was performed.

CD6 molecules were detected at ~100 kDa in Molt-4 cell line under non-reducing conditions.

All lanes:

Western blot - Anti-CD6/T12 antibody [MEM-98] (ab9181) at 1 µg/mL

Lane 1:

Molt-4 whole cell extracts, reducing

Lane 2:

HeLa whole cell extracts, reducing

Lane 3:

Molt-4 whole cell extracts, non-reducing

Lane 4:

HeLa whole cell extracts, non-reducing

Secondary

All lanes:

IRDye 800CW Goat-anti-Mouse IgG

Predicted band size: 72 kDa

false

Key facts

宿主種

Mouse

クローン性

Monoclonal

クローン番号

MEM-98

アイソタイプ

IgG1

軽鎖のタイプ

unknown

キャリアフリー

No

交差種

Human

アプリケーション

WB, Flow Cyt

applications

免疫原

Native Full Length Protein corresponding to Human T-cell differentiation antigen CD6.

P30203

特異性

CD6 / T12 antigen (human)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "2 µg/mL", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "<p>works in WB application under non-reducing conditions</p>" } } }
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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
精製に関する特記事項
Purified from TCS. Purity >95% by SDS-PAGE.
バッファー組成
pH: 7.4 Preservative: 0.097% Sodium azide Constituents: PBS
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

CD6 also known as T12 is a glycoprotein expressed on T cells and some subsets of B cells. The protein has a molecular mass of approximately 105-130 kDa. CD6 is primarily located on the surface of thymocytes mature T lymphocytes and on some B cell lines. It plays an important role in the immunological synapse serving as a receptor that aids in cell-to-cell communication during immune responses. The CD6 protein is also detectable in immune tissues including the spleen and lymph nodes.
Biological function summary

CD6 functions involve T cell maturation and proliferation. The protein is part of the lymphocyte activation complex contributing to the modulation of T cell receptor (TCR) signaling. It interacts with other cell surface proteins such as CD3 and CD28 to influence the response of T cells to antigens. CD6 potentially modulates the threshold for T cell activation favoring the adjustment of immune responses to various antigens.

Pathways

CD6 participates in the signaling pathways that regulate adaptive immune responses. It is involved in the T cell receptor (TCR) signaling pathway essential for T cell activation and differentiation. CD6 interacts with proteins like LCK and ZAP-70 which are pivotal for the TCR signaling cascade. Additionally CD6's role in immune synapse formation connects it to the modulation of cytokine production and immune cell communication.

CD6 associates with autoimmune diseases and multiple sclerosis. The protein's involvement in T cell activation and immune regulation makes it a candidate for studies targeting autoimmune pathologies. Autoimmune disorders such as rheumatoid arthritis have shown links with CD6's activity as its deregulation can lead to inappropriate immune responses. Additionally CD6's interaction with proteins like CD166 has implications in the pathogenesis of these disorders indicating a potential target for therapeutic interventions.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Cell adhesion molecule that mediates cell-cell contacts and regulates T-cell responses via its interaction with ALCAM/CD166 (PubMed : 15048703, PubMed : 15294938, PubMed : 16352806, PubMed : 16914752, PubMed : 24584089, PubMed : 24945728). Contributes to signaling cascades triggered by activation of the TCR/CD3 complex (PubMed : 24584089). Functions as a costimulatory molecule; promotes T-cell activation and proliferation (PubMed : 15294938, PubMed : 16352806, PubMed : 16914752). Contributes to the formation and maturation of the immunological synapse (PubMed : 15294938, PubMed : 16352806). Functions as a calcium-dependent pattern receptor that binds and aggregates both Gram-positive and Gram-negative bacteria. Binds both lipopolysaccharide (LPS) from Gram-negative bacteria and lipoteichoic acid from Gram-positive bacteria (PubMed : 17601777). LPS binding leads to the activation of signaling cascades and down-stream MAP kinases (PubMed : 17601777). Mediates activation of the inflammatory response and the secretion of pro-inflammatory cytokines in response to LPS (PubMed : 17601777).
See full target information T-cell differentiation antigen CD6
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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular medicine 46:1661-1670 PubMed32901835

2020

Expression level of NEAT1 differentiates benign and malignant thyroid nodules by regulating NEAT1/miR‑9/PTEN and NEAT1/miR‑124/PDCD6 signalling.

Applications

Unspecified application

Species

Unspecified reactive species

Li Zhao,Na Zhou,Ping Zhao

Folia biologica 35:289-97 PubMed2534480

1989

Monoclonal antibodies against human leucocyte antigens. III. Antibodies against CD45R, CD6, CD44 and two newly described broadly expressed glycoproteins MEM-53 and MEM-102.

Applications

Unspecified application

Species

Unspecified reactive species

V Bazil,I Stefanová,I Hilgert,H Kristofová,S Vanĕk,A Bukovský,V Horejsí
View all publications
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代替製品

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組合せ製品

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