AB213007

Anti-CD6/T12 抗体 [3F7B5] - BSA and Azide free

Name: Anti-CD6/T12 抗体 [3F7B5] - BSA and Azide free (ab213007) | Abcam
Brand: Abcam Limited
SKU: AB213007
Price: 129000 JPY
Availability: InStock

Anti-CD6/T12 antibody [3F7B5] - BSA and Azide free

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(1 Publication)

Mouse Monoclonal CD6/T12 antibody. Carrier free. Suitable for IHC-P, ICC, Flow Cyt and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Cell preparation containing T-cell differentiation antigen CD6 protein.

別名を表示する

CD6, T-cell differentiation antigen CD6, T12, TP120

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD6/T12 antibody [3F7B5] - BSA and Azide free (AB213007)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD6/T12 antibody [3F7B5] - BSA and Azide free (AB213007)

Immunohistochemical analysis of formalix-fixed, paraffin-embedded Human tonsil tissue, labeling CD6/T12 using ab213007 at 1 μg/mL.

Unknown

Immunocytochemistry - Anti-CD6/T12 antibody [3F7B5] - BSA and Azide free (AB213007)

Immunofluorescence staining of MOLT-4 cells labeling CD6/T12 with ab213007 followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are stained with Reddot.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD6/T12 antibody [3F7B5] - BSA and Azide free (AB213007)

Immunohistochemical analysis of formalix-fixed, paraffin-embedded Human tonsil tissue, labeling CD6/T12 using ab213007 at 1 μg/mL.

Flow Cytometry - Anti-CD6/T12 antibody [3F7B5] - BSA and Azide free (AB213007)

Flow Cyt

Unknown

Flow Cytometry - Anti-CD6/T12 antibody [3F7B5] - BSA and Azide free (AB213007)

This image was produced using ab193341, the same clone but in a different formulation. Flow Cytometric analysis of MOLT-4 (human lymphoblastic leukemia cell line) cells staining CD6/T12 using ab213007 at 2ug/million cells, followed by goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red).

Key facts

宿主種

Mouse

クローン性

Monoclonal

クローン番号

3F7B5

アイソタイプ

IgG1

キャリアフリー

Yes

交差種

Human

アプリケーション

Flow Cyt, IHC-P, ICC

applications

免疫原

Cell preparation containing T-cell differentiation antigen CD6 protein. The exact immunogen used to generate this antibody is proprietary information.

P30203

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1-2 µg/mL", "IHCP-species-notes": "Primary incubation for 30 minutes at room temperature.", "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "1-2 µg/mL", "ICC-species-notes": "", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1-2 µg for 10^6 Cells", "FlowCyt-species-notes": "" } } }

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein G

精製に関する特記事項

ab213007 was purified from Bioreactor Concentrate by Protein A/G.

バッファー組成

pH: 7.2 - 7.4 Constituents: PBS

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

CD6 also known as T12 is a glycoprotein expressed on T cells and some subsets of B cells. The protein has a molecular mass of approximately 105-130 kDa. CD6 is primarily located on the surface of thymocytes mature T lymphocytes and on some B cell lines. It plays an important role in the immunological synapse serving as a receptor that aids in cell-to-cell communication during immune responses. The CD6 protein is also detectable in immune tissues including the spleen and lymph nodes.

Biological function summary

CD6 functions involve T cell maturation and proliferation. The protein is part of the lymphocyte activation complex contributing to the modulation of T cell receptor (TCR) signaling. It interacts with other cell surface proteins such as CD3 and CD28 to influence the response of T cells to antigens. CD6 potentially modulates the threshold for T cell activation favoring the adjustment of immune responses to various antigens.

Pathways

CD6 participates in the signaling pathways that regulate adaptive immune responses. It is involved in the T cell receptor (TCR) signaling pathway essential for T cell activation and differentiation. CD6 interacts with proteins like LCK and ZAP-70 which are pivotal for the TCR signaling cascade. Additionally CD6's role in immune synapse formation connects it to the modulation of cytokine production and immune cell communication.

CD6 associates with autoimmune diseases and multiple sclerosis. The protein's involvement in T cell activation and immune regulation makes it a candidate for studies targeting autoimmune pathologies. Autoimmune disorders such as rheumatoid arthritis have shown links with CD6's activity as its deregulation can lead to inappropriate immune responses. Additionally CD6's interaction with proteins like CD166 has implications in the pathogenesis of these disorders indicating a potential target for therapeutic interventions.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Cell adhesion molecule that mediates cell-cell contacts and regulates T-cell responses via its interaction with ALCAM/CD166 (PubMed : 15048703, PubMed : 15294938, PubMed : 16352806, PubMed : 16914752, PubMed : 24584089, PubMed : 24945728). Contributes to signaling cascades triggered by activation of the TCR/CD3 complex (PubMed : 24584089). Functions as a costimulatory molecule; promotes T-cell activation and proliferation (PubMed : 15294938, PubMed : 16352806, PubMed : 16914752). Contributes to the formation and maturation of the immunological synapse (PubMed : 15294938, PubMed : 16352806). Functions as a calcium-dependent pattern receptor that binds and aggregates both Gram-positive and Gram-negative bacteria. Binds both lipopolysaccharide (LPS) from Gram-negative bacteria and lipoteichoic acid from Gram-positive bacteria (PubMed : 17601777). LPS binding leads to the activation of signaling cascades and down-stream MAP kinases (PubMed : 17601777). Mediates activation of the inflammatory response and the secretion of pro-inflammatory cytokines in response to LPS (PubMed : 17601777).

See full target information T-cell differentiation antigen CD6

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Cell reports methods 3:100595 PubMed37741277

2023

Dual-modality imaging of immunofluorescence and imaging mass cytometry for whole-slide imaging and accurate segmentation.

Applications

Unspecified application

Species

Unspecified reactive species

Eun Na Kim,Phyllis Zixuan Chen,Dario Bressan,Monika Tripathi,Ahmad Miremadi,Massimiliano di Pietro,Lisa M Coussens,Gregory J Hannon,Rebecca C Fitzgerald,Lizhe Zhuang,Young Hwan Chang

View all publications

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代替製品

Primary Antibodies

AB193341

Anti-CD6/T12 antibody [3F7B5]

Immunocytochemistry - Anti-CD6/T12 antibody [3F7B5] (AB193341)

Primary Antibodies

AB9181

Anti-CD6/T12 antibody [MEM-98]

Flow Cytometry - Anti-CD6/T12 antibody [MEM-98] (AB9181)

Primary Antibodies

AB204949

Anti-CD6/T12 antibody [5A10A2]

Flow Cytometry - Anti-CD6/T12 antibody [5A10A2] (AB204949)

Primary Antibodies

AB231314

Anti-CD6/T12 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD6/T12 antibody (AB231314)

Primary Antibodies

AB109217

Anti-CD6/T12 antibody [EPR4057]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD6/T12 antibody [EPR4057] (AB109217)

Primary Antibodies

AB199008

Anti-CD6/T12 antibody [C6/372 + 3F7B5]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD6/T12 antibody [C6/372 + 3F7B5] (AB199008)

Primary Antibodies

AB109218

Anti-CD6/T12 antibody [EPR4056]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD6/T12 antibody [EPR4056] (AB109218)

組合せ製品

Assay Kits

AB313526

Human CD6/T12 ELISA Kit

Sandwich ELISA - Human CD6/T12 ELISA Kit (AB313526)

Secondary Antibodies

AB150113

Goat Anti-Mouse IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (AB150113)

Primary Antibodies

AB237709

Anti-CD8 alpha antibody [CAL66]

BOND RX™ Validated|20ul selling size|RabMAb|Advanced Validation|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 alpha antibody [CAL66] (AB237709)

Primary Antibodies

AB125011

Anti-TSG101 antibody [EPR7130(B)]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry - Anti-TSG101 antibody [EPR7130(B)] (AB125011)

Primary Antibodies

AB32391

Anti-GSK3 beta antibody [Y174]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GSK3 beta antibody [Y174] (AB32391)

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