Anti-CD59 抗体 [EPR6425(2)] (ab133707)
![Western blot - Anti-CD59 antibody [EPR6425(2)] (ab133707)](https://www.abcam.co.jp/ps/products/133/ab133707/Images/ab133707-304206-ab133707KOWBMPES.jpg "Western blot - Anti-CD59 antibody [EPR6425(2)] (ab133707)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6425(2)] to CD59
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
製品の概要
-
製品名
Anti-CD59 antibody [EPR6425(2)]
CD59 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR6425(2)] to CD59 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details
適用なし: Flow Cyt,ICC/IF or IP -
種交差性
交差種: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
ポジティブ･コントロール
- HUVEC and BxPC3 cell lysates; Human placenta and tonsil tissues
-
特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR6425(2) -
アイソタイプ
IgG -
研究分野
関連製品
-
Alternative Versions
- Anti-CD59 antibody [EPR6425(2)] - BSA and Azide free (ab248625)
- APC Anti-CD59 antibody [EPR6425(2)] (ab319256)
- PE Anti-CD59 antibody [EPR6425(2)] (ab319404)
- Alexa Fluor® 488 Anti-CD59 antibody [EPR6425(2)] (ab319538)
- Alexa Fluor® 647 Anti-CD59 antibody [EPR6425(2)] (ab319651)
- Alexa Fluor® 594 Anti-CD59 antibody [EPR6425(2)] (ab319755)
- Alexa Fluor® 555 Anti-CD59 antibody [EPR6425(2)] (ab319897)
- Alexa Fluor® 750 Anti-CD59 antibody [EPR6425(2)] (ab321064)
-
Isotype control
-
Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab133707の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 14 kDa.
|
|
| IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| 特記事項 |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 14 kDa. |
|
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
-
機能
Potent inhibitor of the complement membrane attack complex (MAC) action. Acts by binding to the C8 and/or C9 complements of the assembling MAC, thereby preventing incorporation of the multiple copies of C9 required for complete formation of the osmolytic pore. This inhibitor appears to be species-specific. Involved in signal transduction for T-cell activation complexed to a protein tyrosine kinase.
The soluble form from urine retains its specific complement binding activity, but exhibits greatly reduced ability to inhibit MAC assembly on cell membranes. -
関連疾患
Defects in CD59 are the cause of CD59 deficiency (CD59D) [MIM:612300]. -
配列類似性
Contains 1 UPAR/Ly6 domain. -
翻訳後修飾
N- and O-glycosylated. The N-glycosylation mainly consists of a family of biantennary complex-type structures with and without lactosamine extensions and outer arm fucose residues. Also significant amounts of triantennary complexes (22%). Variable sialylation also present in the Asn-43 oligosaccharide. The predominant O-glycans are mono-sialylated forms of the disaccharide, Gal-beta-1,3GalNAc, and their sites of attachment are probably on Thr-76 and Thr-77. The GPI-anchor of soluble urinary CD59 has no inositol-associated phospholipid, but is composed of seven different GPI-anchor variants of one or more monosaccharide units. Major variants contain sialic acid, mannose and glucosamine Sialic acid linked to an N-acetylhexosamine-galactose arm is present in two variants.
Glycated. Glycation is found in diabetic subjects, but only at minimal levels in nondiabetic subjects. Glycated CD59 lacks MAC-inhibitory function and confers to vascular complications of diabetes. -
細胞内局在
Cell membrane. Secreted. Soluble form found in a number of tissues. - Information by UniProt
-
参照データベース
- Entrez Gene: 966 Human
- Omim: 107271 Human
- SwissProt: P13987 Human
- Unigene: 278573 Human
- Unigene: 709466 Human
- Unigene: 710641 Human
-
別名
- 16.3A5 antibody
- 1F5 antibody
- 1F5 antigen antibody
see all
画像
-
Western blot - Anti-CD59 antibody [EPR6425(2)] (ab133707)
Lane 1: Wild-type HAP1 whole cell lysate (40 µg)
Lane 2: CD59 knockout HAP1 whole cell lysate (40 µg)Lanes 1 - 2: Merged signal (red and green). Green - ab133707 observed at 14 kDa. Red - loading control, ab9484, observed at 37 kDa.
ab133707 was shown to specifically react with CD59 in wild-type HAP1 cells as signal was lost in CD59 knockout cells. Wild-type and CD59 knockout samples were subjected to SDS-PAGE. Ab133707 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
-
![Western blot - Anti-CD59 antibody [EPR6425(2)] (ab133707)](https://www.abcam.co.jp/ps/products/133/ab133707/Images/CD59-Primary-antibodies-ab133707-1.jpg)
Western blot - Anti-CD59 antibody [EPR6425(2)] (ab133707)All lanes : Anti-CD59 antibody [EPR6425(2)] (ab133707) at 1/1000 dilution
Lane 1 : HUVEC cell lysate
Lane 2 : BxPC 3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution
Predicted band size: 14 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD59 antibody [EPR6425(2)] (ab133707)](https://www.abcam.co.jp/ps/products/133/ab133707/Images/ab133707-147732-anti-cd59-antibody-epr64252-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD59 antibody [EPR6425(2)] (ab133707)Immunohistochemical analysis of CD59 in paraffin embedded Human placenta tissue labelled with ab133707 at a 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD59 antibody [EPR6425(2)] (ab133707)](https://www.abcam.co.jp/ps/products/133/ab133707/Images/ab133707-147731-anti-cd59-antibody-epr64252-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD59 antibody [EPR6425(2)] (ab133707)Immunohistochemical analysis of CD59 in paraffin embedded Human tonsil tissue labelled with ab133707 at a 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
![Anti-CD59 antibody [EPR6425(2)] (ab133707)](https://www.abcam.co.jp/ps/products/133/ab133707/Images/ab133707-5-benefits-of-recombinant-antibodies.png)
Anti-CD59 antibody [EPR6425(2)] (ab133707)
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (4)
ab133707 は 4 報の論文で使用されています。
- Ge X et al. Complement and complement regulatory proteins are upregulated in lungs of COVID-19 patients. Pathol Res Pract 247:154519 (2023). PubMed: 37244049
- Chen Y et al. Engineered extracellular vesicles for concurrent Anti-PDL1 immunotherapy and chemotherapy. Bioact Mater 9:251-265 (2022). PubMed: 34820569
- Clark AJ & Petty HR Identification of lesion subtypes in biopsies of ductal carcinoma in situ of the breast using biomarker ratio imaging microscopy. Sci Rep 6:27039 (2016). PubMed: 27247112
- Clark AJ & Petty HR Protocol for Biomarker Ratio Imaging Microscopy with Specific Application to Ductal Carcinoma In situ of the Breast. Front Cell Dev Biol 4:120 (2016). PubMed: 27857940