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AB22383

Anti-CD53 抗体 [MRC OX-44]

Anti-CD53 antibody [MRC OX-44]

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(2 Publications)

Mouse Monoclonal CD53 antibody. Suitable for Flow Cyt and reacts with Rat samples. Cited in 2 publications.

別名を表示する

CD53, MOX44, TSPAN25, Leukocyte surface antigen CD53, Cell surface glycoprotein CD53, Tetraspanin-25, Tspan-25

1 Images
Flow Cytometry - Anti-CD53 antibody [MRC OX-44] (AB22383)
  • Flow Cyt

Lab

Flow Cytometry - Anti-CD53 antibody [MRC OX-44] (AB22383)

Lewis rat splenocytes stained with ab22383 (right) or mouse IgG1κ (left). Lewis rat splenocytes were incubated for 30 min on ice in 10% rat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab22383) or mouse IgG1κ Isotype (ab170190) (1x106 in 100μl at 0.2 μg/ml) for 30 min on ice.

The secondary antibody Goat Anti-Mouse IgG H&L (Alexa Fluor ® 488, pre-adsorbed) (ab150117) was used at 1/2000 dilution for 30 min at 4°C. The cells were simultaneously stained with CD3 APC antibody.

Acquisition of >30,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter. Events were gated on viable lymphocytes.

関連する標識済み抗体及び組成の異なる製品 (2)

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-CD53 antibody [MRC OX-44]

  • 660 APC

    APC Anti-CD53 antibody [MRC OX-44]

Key facts

宿主種

Mouse

クローン性

Monoclonal

クローン番号

MRC OX-44

アイソタイプ

IgG1

軽鎖のタイプ

kappa

キャリアフリー

No

交差種

Rat

アプリケーション

Flow Cyt

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Rat": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "0.2 µg/mL", "FlowCyt-species-notes": "<p></p>" } } }
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製品の詳細

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification
精製に関する特記事項
Purified from TCS.
バッファー組成
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

CD53 also known as Tetraspanin-25 is a transmembrane glycoprotein with a molecular mass of approximately 35-40 kDa. It belongs to the tetraspanin family characterized by four transmembrane domains. CD53 primarily resides on the surface of leukocytes including B cells T cells natural killer cells and macrophages. This protein plays an important role in signal transduction processes by organizing proteins on the cell membrane and forming tetraspanin-enriched microdomains.
Biological function summary

CD53 plays a significant role in regulating immune responses cell development and migration. It does not work alone but as part of larger protein complexes that influence cell communication and inter-organizational responses. CD53 participates in organizing membrane proteins into functional complexes which impacts cellular processes such as adhesion signaling and cellular activation. Its interaction with other proteins is critical to maintain normal immune system functionality and responding appropriately to infections.

Pathways

CD53 is involved in the regulation of immune signaling and cellular communication networks. It is a component of the tetraspanin web which influences the integrin and immunoreceptor pathways two important signaling pathways in cellular communication. Proteins such as CD9 and CD81 also members of the tetraspanin family frequently interact with CD53 suggesting cooperative roles in modulating cellular adhesion and trafficking processes critical for immune response.

CD53 shows a connection to immune system dysfunctions including autoimmune conditions and certain types of cancer. Its altered expression can contribute to abnormal immune responses seen in diseases like systemic lupus erythematosus (SLE) and lymphoma. In these disorders CD53's interaction with CD19 and other B-cell-associated proteins plays a part in abnormal cell signaling leading to issues in cell proliferation or miscommunication within the immune system.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Structural component of specialized membrane microdomains known as tetraspanin-enriched microdomains (TERMs), which act as platforms for receptor clustering and signaling (PubMed : 28487417). Participates thereby in diverse biological functions such as cell signal transduction, adhesion, migration and protein trafficking (PubMed : 32974937, PubMed : 35767951). Plays a role in the activation of monocytes and B-cells (PubMed : 8335905). Acts as an essential regulator of B-cell development by promoting interleukin-7 receptor/IL7R signaling (By similarity). Promotes also in B-cells the BCR signaling by recruiting PKC to the plasma membrane in order to phosphorylate its substrates (PubMed : 28487417). Plays an essential role in B- and T-cells homing to lymph nodes by stabilizing L-selectin/SELL cell surface expression (By similarity). Mediates also metabolic and inflammatory functions in hepatocytes and adipose tissue by promoting TNF-alpha and LPS signaling independent of the immune compartment (By similarity).
See full target information CD53
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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

European journal of immunology 23:136-40 PubMed7678222

1993

Epitope mapping of anti-rat CD53 monoclonal antibodies. Implications for the membrane orientation of the Transmembrane 4 Superfamily.

Applications

Unspecified application

Species

Unspecified reactive species

M G Tomlinson,A F Williams,M D Wright

The Journal of experimental medicine 165:1-13 PubMed3098892

1987

The MRC OX-44 antigen marks a functionally relevant subset among rat thymocytes.

Applications

Unspecified application

Species

Unspecified reactive species

D J Paterson,J R Green,W A Jefferies,M Puklavec,A F Williams
View all publications
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