JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB239796

Anti-CD5 抗体 [SP19] - BSA and Azide free

Anti-CD5 antibody [SP19] - BSA and Azide free

4

(1 Review)

|

(2 Publications)

Rabbit Recombinant Monoclonal CD5 antibody. Carrier free. Suitable for IHC-P and reacts with Human samples. Cited in 2 publications.

別名を表示する

CD5, LEU1, T-cell surface glycoprotein CD5, Lymphocyte antigen T1/Leu-1

1 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD5 antibody [SP19] - BSA and Azide free (AB239796)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD5 antibody [SP19] - BSA and Azide free (AB239796)

ab16699 at a dilution of 1/50 staining CD5 in formalin fixed paraffin embedded human tonsil tissue section by Immunohistochemistry.

This data was developed using the same antibody clone in a different buffer formulation containing Tris buffered saline, BSA, and sodium azide (ab16699).

関連する標識済み抗体及び組成の異なる製品 (2)

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

SP19

アイソタイプ

IgG

キャリアフリー

Yes

交差種

Human

アプリケーション

IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

エピトープ

ab239796 reacts with an epitope located in the intracellular region of CD5.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
style
left-column

製品の詳細

ab239796 is the carrier-free version of ab16699.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

style
left-column

出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification
バッファー組成
pH: 7.2 - 7.4 Constituents: PBS
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
+4°C
保管に関する情報
Do Not Freeze
style
left-column

補足情報

This supplementary information is collated from multiple sources and compiled automatically.

CD5 also known as T1 or Leu-1 is a glycoprotein with a molecular mass of approximately 67 kDa. It is expressed mainly on the surface of T cells and a subset of B cells. CD5 plays a major role in the regulation of immune responses. It functions by modulating T-cell receptor (TCR) signaling often acting as a negative regulator to prevent excessive immune activation. Researchers often utilize CD5 antibodies such as those conjugated with PerCP for immunohistochemistry (IHC) to study this protein's expression and distribution.
Biological function summary

CD5 is essential in the immune system's ability to maintain tolerance to self-antigens thereby preventing autoimmune responses. CD5 forms part of a receptor complex on the cell surface that interacts with ligands and other receptors to fine-tune immune cell signaling. Its localization and function in T cells relate closely to its ability to modulate signaling pathways essential for cell survival and proliferation. This modulation is important for ensuring that immune responses are appropriate to the stimuli encountered.

Pathways

CD5 is involved in signal transduction processes important for immune tolerance and modulation. It intersects with pathways like the T cell receptor (TCR) signaling pathway and the NF-kB signaling pathway. These pathways involve interaction with proteins like Zap70 a tyrosine kinase related to TCR signaling and the downstream activation of transcription factors that regulate immune responses. The protein CRIS1 also appears in some pathway interactions highlighting the interconnected nature of immune regulatory proteins.

CD5 has significant associations with autoimmune diseases such as systemic lupus erythematosus (SLE) where its modulatory role can affect autoantibody production. Additionally CD5 in conjunction with proteins like OX19 correlates with certain lymphoproliferative disorders including chronic lymphocytic leukemia (CLL). In these disorders the expression and function of CD5 may impact disease progression and patient response to therapy making it a potential target for intervention in immune-related conditions.
style
left-column
style
left-column

製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

ターゲットの情報

Lymphoid-specific receptor expressed by all T-cells and in a subset of B-cells known as B1a cells. Plays a role in the regulation of TCR and BCR signaling, thymocyte selection, T-cell effector differentiation and immune tolerance. Acts by interacting with several ligands expressed on B-cells such as CD5L or CD72 and thereby plays an important role in contact-mediated, T-dependent B-cell activation and in the maintenance of regulatory T and B-cell homeostasis. Functions as a negative regulator of TCR signaling during thymocyte development by associating with several signaling proteins including LCK, CD3Z chain, PI3K or CBL (PubMed : 1384049, PubMed : 1385158). Mechanistically, co-engagement of CD3 with CD5 enhances phosphorylated CBL recruitment leading to increased VAV1 phosphorylation and degradation (PubMed : 23376399). Modulates B-cell biology through ERK1/2 activation in a Ca(2+)-dependent pathway via the non-selective Ca(2+) channel TRPC1, leading to IL-10 production (PubMed : 27499044).
See full target information CD5
style
left-column

文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of cellular and molecular medicine 29:e70753 PubMed40801794

2025

TRIM47 Facilitates Osteosarcoma Progression via Destabilising FBP1 and Thus Activation of Wnt/β-Catenin Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Heng Wang,Xiao Chen,Fengting Nie,Min Zhong,Zhi Fang,Zezhi Qiu,Ling Zhou,Yi Le,Xianpin Wei,Yanyu Liao,Ziling Fang

Nature communications 16:6009 PubMed40593805

2025

Spatial and single cell mapping of castleman disease reveals key stromal cell types and cytokine pathways.

Applications

Unspecified application

Species

Unspecified reactive species

David Smith,Anna Eichinger,Éanna Fennell,Zijun Y Xu-Monette,Andrew Rech,Julia Wang,Eduardo Esteva,Arta Seyedian,Xiaoxu Yang,Mei Zhang,Dan Martinez,Kai Tan,Minjie Luo,Katherine J Young,Paul G Murray,Christopher Park,Boris Reizis,Vinodh Pillai
View all publications
style
left-column
style
left-column
style
right-column

代替製品

Primary Antibodies

AB213001

Anti-CD5 antibody [C5/473] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD5 antibody [C5/473] - BSA and Azide free (AB213001)

  • 0
  • 0
Primary Antibodies

AB213003

Anti-CD5 antibody [CD5/54/F6] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD5 antibody [CD5/54/F6] - BSA and Azide free (AB213003)

  • 0
  • 0
Primary Antibodies

AB213004

Anti-CD5 antibody [C5/473 + CD5/54/F6] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD5 antibody [C5/473 + CD5/54/F6] - BSA and Azide free (AB213004)

  • 0
  • 0
Primary Antibodies

AB237940

Anti-CD5 antibody [CD5/2416] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD5 antibody [CD5/2416] - BSA and Azide free (AB237940)

  • 0
  • 0
Primary Antibodies

AB200697

Anti-CD5 antibody [C5/473 + CD5/54/F6]

  • 0
  • 0
Primary Antibodies

AB201338

Anti-CD5 antibody [C5/473]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD5 antibody [C5/473] (AB201338)

  • 0
  • 0
Primary Antibodies

AB238086

Anti-CD5 antibody [CD5/2416]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD5 antibody [CD5/2416] (AB238086)

  • 0
  • 0
Primary Antibodies

AB232704

PE/Cy7® Anti-CD5 antibody [L17F12]

Flow Cytometry - PE/Cy7® Anti-CD5 antibody [L17F12] (AB232704)

  • 0
  • 0
Primary Antibodies

AB234277

PerCP/Cy5.5® Anti-CD5 antibody [L17F12]

Flow Cytometry - PerCP/Cy5.5® Anti-CD5 antibody [L17F12] (AB234277)

  • 0
  • 0
Primary Antibodies

AB234243

PE/Cy5® Anti-CD5 antibody [L17F12]

Flow Cytometry - PE/Cy5® Anti-CD5 antibody [L17F12] (AB234243)

  • 0
  • 0
Primary Antibodies

AB52310

FITC Anti-CD5 antibody [M28623]

  • 0
  • 0
Primary Antibodies

AB218754

APC Anti-CD5 antibody [53-7.3]

Flow Cytometry - APC Anti-CD5 antibody [53-7.3] (AB218754)

  • 0
  • 0
Primary Antibodies

AB114048

FITC Anti-CD5 antibody [53-7.3]

Flow Cytometry - FITC Anti-CD5 antibody [53-7.3] (AB114048)

  • 0
  • 0
Primary Antibodies

AB27371

Anti-CD5 antibody [B-B8]

Flow Cytometry - Anti-CD5 antibody [B-B8] (AB27371)

  • 0
  • 0
Primary Antibodies

AB269710

Anti-CD5 antibody [B-B8] - BSA and Azide free

Flow Cytometry - Anti-CD5 antibody [B-B8] - BSA and Azide free (AB269710)

  • 0
  • 0

組合せ製品

Assay Kits

AB309429

Human CD5 ELISA Kit

Sandwich ELISA - Human CD5 ELISA Kit (AB309429)

  • 0
  • 0
Primary Antibodies

AB64088

Anti-CD20 antibody [SP32]

BOND RX™ Validated|20ul selling size|RabMAb|Advanced Validation|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD20 antibody [SP32] (AB64088)

  • 5
  • 12
Primary Antibodies

AB10558

Anti-CD45 antibody

BOND RX™ Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD45 antibody (AB10558)

  • 4
  • 63
Primary Antibodies

AB16698

Anti-CD79a antibody [SP18]

20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD79a antibody [SP18] (AB16698)

  • 1
  • 1
Primary Antibodies

AB133616

Anti-CD4 antibody [EPR6855]

BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size|Advanced Validation

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD4 antibody [EPR6855] (AB133616)

  • 5
  • 20

Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。
保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com