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リコンビナントRabMAb

Anti-CD5 抗体 [EPR26533-75] (BSA and Azide free) (ab300424)

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Western blot - Anti-CD5 antibody [EPR26533-75] (BSA and Azide free) (ab300424)
  • Western blot - Rabbit monoclonal [EPR26533-75] to CD5 - BSA and Azide free (AB300424)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit monoclonal [EPR26533-75] to CD5 - BSA and Azide free (AB300424)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit monoclonal [EPR26533-75] to CD5 - BSA and Azide free (AB300424)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit monoclonal [EPR26533-75] to CD5 - BSA and Azide free (AB300424)
  • Immunoprecipitation - Rabbit monoclonal [EPR26533-75] to CD5 - BSA and Azide free (AB300424)
  • Rabbit monoclonal [EPR26533-75] to CD5 - BSA and Azide free (AB300424)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR26533-75] to CD5 - BSA and Azide free
  • Suitable for: WB, IHC-P, IP
  • Reacts with: Mouse, Rat

Conjugates logo Related conjugates and formulations

Alexa Fluor® 647 Unconjugated

製品の概要

  • 製品名

    Anti-CD5 antibody [EPR26533-75] (BSA and Azide free)
    CD5 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR26533-75] to CD5 - BSA and Azide free

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IHC-P, IPmore details
    適用なし: Flow Cyt or ICC/IF

  • 種交差性

    交差種: Mouse, Rat
    非交差種: Human

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: EL4, A20 whole cell lysates; mouse spleen, skeletal muscle tissue lysates; rat spleen, rat skeletal muscle tissue lysates. IHC-P: Mouse and rat spleen FFPE tissue sections. IP: EL4 whole cell lysate.

  • 特記事項

    ab300424 is the carrier-free version of ab300423. 

     

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab300424の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IP
Use at an assay dependent concentration.
特記事項
WB
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IP
Use at an assay dependent concentration.
追加情報
Is unsuitable for Flow Cyt or ICC/IF.

ターゲット情報

画像

  • Western blot - Anti-CD5 antibody [EPR26533-75] (BSA and Azide free) (ab300424)
    Western blot - Anti-CD5 antibody [EPR26533-75] (BSA and Azide free) (ab300424)
    All lanes : Anti-CD5 antibody [EPR26533-75] (ab300423) at 1/1000 dilution

    Lane 1 : EL4 (mouse lymphoma T lymphocyte), whole cell lysate
    Lane 2 : A20 (mouse reticulum sarcoma B lymphocyte), whole cell lysate,

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Observed band size: 67 kDa why is the actual band size different from the predicted?


    Exposure time: 37 seconds


    This data was developed using ab300423, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Negative control: A20 (PMID:19520434).

    This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

  • Western blot - Rabbit monoclonal [EPR26533-75] to CD5 - BSA and Azide free (AB300424)
    Western blot - Rabbit monoclonal [EPR26533-75] to CD5 - BSA and Azide free (AB300424)
    Lanes 2-4 : Anti-CD5 antibody [EPR26533-75] (ab300423) at 1/1000 dilution

    Lane 1 : Mouse spleen tissue lysate
    Lane 2 : Mouse skeletal muscle tissue lysate
    Lane 3 : Rat spleen tissue lysate
    Lane 4 : Rat skeletal muscle tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Observed band size: 67 kDa why is the actual band size different from the predicted?



    This data was developed using ab300423, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Negative control: Skeleal muscle (HPA)

    Exposure time: 147 seconds

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit monoclonal [EPR26533-75] to CD5 - BSA and Azide free (AB300424)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit monoclonal [EPR26533-75] to CD5 - BSA and Azide free (AB300424)

    This data was developed using ab300423, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue labeling CD5 with ab300423 at 1/5000 dilution (0.104 µg/mL) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection kit). Negative control: no staining on mouse skeletal muscle. The section was incubated with ab300423 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: PBS was used instead of primary antibody, followed by a ready to use secondary antibody LeicaDS9800 (Bond™ Polymer Refine Detection kit).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit monoclonal [EPR26533-75] to CD5 - BSA and Azide free (AB300424)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit monoclonal [EPR26533-75] to CD5 - BSA and Azide free (AB300424)

    This data was developed using ab300423, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded rat spleen tissue labeling CD5 with ab300423 at 1/5000 dilution (0.104 µg/mL) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection kit). Membranous staining on rat spleen. The section was incubated with ab300423 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: PBS was used instead of primary antibody, followed by a ready to use secondary antibody LeicaDS9800 (Bond™ Polymer Refine Detection kit).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit monoclonal [EPR26533-75] to CD5 - BSA and Azide free (AB300424)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit monoclonal [EPR26533-75] to CD5 - BSA and Azide free (AB300424)

    This data was developed using ab300423, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling CD5 with ab300423 at 1/5000 dilution (0.104 µg/mL) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection kit). Membranous staining on mouse spleen. The section was incubated with ab300423 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: PBS was used instead of primary antibody, followed by a ready to use secondary antibody LeicaDS9800 (Bond™ Polymer Refine Detection kit).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

  • Immunoprecipitation - Rabbit monoclonal [EPR26533-75] to CD5 - BSA and Azide free (AB300424)
    Immunoprecipitation - Rabbit monoclonal [EPR26533-75] to CD5 - BSA and Azide free (AB300424)

    This data was developed using ab300423, the same antibody clone in a different buffer formulation.

    CD5 was immunoprecipitated from 0.35 mg EL4 (mouse lymphoma T lymphocyte) whole cell lysate 10 µg with ab300423 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab300423 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: EL4 (mouse lymphoma T lymphocyte) whole cell lysate, 10 µg

    Lane 2: ab300423 IP in EL4 (mouse lymphoma T lymphocyte) whole cell lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab300424 in EL4 whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST

    Exposure time: 10 seconds

  • Rabbit monoclonal [EPR26533-75] to CD5 - BSA and Azide free (AB300424)
    Rabbit monoclonal [EPR26533-75] to CD5 - BSA and Azide free (AB300424)

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (0)

ab300424 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab300424 は論文での使用が確認できていません。

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