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リコンビナントRabMAb

Anti-CD5 抗体 [EPR26532-12] (BSA and Azide free) (ab300145)

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Immunocytochemistry/ Immunofluorescence - Anti-CD5 antibody [EPR26532-12] (BSA and Azide free) (ab300145)
  • Immunocytochemistry/ Immunofluorescence - Anti-CD5 antibody [EPR26532-12] (BSA and Azide free) (ab300145)
  • Flow Cytometry - Anti-CD5 antibody [EPR26532-12] (BSA and Azide free) (AB300145)
  • Flow Cytometry - Anti-CD5 antibody [EPR26532-12] (BSA and Azide free) (AB300145)
  • Anti-CD5 antibody [EPR26532-12] (BSA and Azide free) (AB300145)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR26532-12] to CD5 - BSA and Azide free
  • Suitable for: Flow Cyt, ICC/IF
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 647 Unconjugated

製品の概要

  • 製品名

    Anti-CD5 antibody [EPR26532-12] (BSA and Azide free)
    CD5 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR26532-12] to CD5 - BSA and Azide free

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: Flow Cyt, ICC/IFmore details
    適用なし: IHC-P,IP or WB

  • 種交差性

    交差種: Human
    非交差種: Mouse, Rat

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • ICC/IF: MOLT-4 and Human PBMC cellss. Flow Cyt.: Human peripheral blood mononuclear.

  • 特記事項

    ab300145 is a carrier free version of ab300144. 

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab300145の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
特記事項
Flow Cyt
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
追加情報
Is unsuitable for IHC-P,IP or WB.

ターゲット情報

  • 機能

    May act as a receptor in regulating T-cell proliferation. CD5 interacts with CD72/LYB-2.

  • 配列類似性

    Contains 3 SRCR domains.

  • 細胞内局在

    Membrane.
  • Information by UniProt

  • 参照データベース

    • 別名

      • CD 5 antibody
      • CD5 antibody
      • CD5 antigen (p56 62) antibody
      • CD5 antigen antibody
      • CD5 molecule antibody
      • CD5_HUMAN antibody
      • LEU1 antibody
      • LyA antibody
      • Lymphocyte antigen T1/Leu-1 antibody
      • OTTHUMP00000236973 antibody
      • T-cell surface glycoprotein CD5 antibody
      • T1 antibody
      see all

    画像

    • Immunocytochemistry/ Immunofluorescence - Anti-CD5 antibody [EPR26532-12] (BSA and Azide free) (ab300145)
      Immunocytochemistry/ Immunofluorescence - Anti-CD5 antibody [EPR26532-12] (BSA and Azide free) (ab300145)

      This data was developed using ab300144, the same antibody clone in a different buffer formulation.

      Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeilized MOLT-4 (human lymphoblastic leukemia T lymphoblast) cells lebelling CD5 with ab300144 at 1/100 (5.04 µg/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 µg/ml dilution (Green). Confocal image showing membranous staining in MOLT-4 cell line is observed. Negative control: HeLa (PMID:15187131). ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5µg/ml dilution (Red). The Nuclear counterstain was DAPI (Blue).

      Secondary antibody only control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 µg/ml) dilution.

    • Immunocytochemistry/ Immunofluorescence - Anti-CD5 antibody [EPR26532-12] (BSA and Azide free) (ab300145)
      Immunocytochemistry/ Immunofluorescence - Anti-CD5 antibody [EPR26532-12] (BSA and Azide free) (ab300145)

      This data was developed using ab300144, the same antibody clone in a different buffer formulation.

      Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeilized Human PBMC (human primary peripheral blood mononuclear cell ) cells lebelling CD5 with ab300144 at 1/100 (5.04 µg/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 µg/ml) dilution (Green). Confocal image showing membranous staining in subsets of human PBMC is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5µg/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).

      Secondary antibody only control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 µg/ml) dilution.

    • Flow Cytometry - Anti-CD5 antibody [EPR26532-12] (BSA and Azide free) (AB300145)
      Flow Cytometry - Anti-CD5 antibody [EPR26532-12] (BSA and Azide free) (AB300145)

      This data was developed using ab300144, the same antibody clone in a different buffer formulation.

      Flow cytometric analysis of Human peripheral blood mononuclear cell (PBMC) cells labelling CD5 with ab300144 at 1/500 dilution (0.1µg)/ Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control. Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody. Gated on viable cells.Cells were stained with rabbit IgG or ab300144. Then stained with anti-CD3 conjugated to Alexa Fluor® 647.

    • Flow Cytometry - Anti-CD5 antibody [EPR26532-12] (BSA and Azide free) (AB300145)
      Flow Cytometry - Anti-CD5 antibody [EPR26532-12] (BSA and Azide free) (AB300145)

      This data was developed using ab300144, the same antibody clone in a different buffer formulation.

      Flow cytometric analysis of Human peripheral blood mononuclear cell (PBMC) cells labelling CD5 with ab300144 at 1/500 dilution (0.1µg)/ Right (Red) compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control. A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody. Gated on viable cells.

    • Anti-CD5 antibody [EPR26532-12] (BSA and Azide free) (AB300145)
      Anti-CD5 antibody [EPR26532-12] (BSA and Azide free) (AB300145)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab300145 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab300145 は論文での使用が確認できていません。

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