Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
リコンビナントRabMAb

Anti-CD5 抗体 [EP2952] (ab75877)

Submit a review Q&A (1)References (3)
Immunoprecipitation - Anti-CD5 antibody [EP2952] (ab75877)
  • Western blot - Anti-CD5 antibody [EP2952] (ab75877)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD5 antibody [EP2952] (ab75877)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD5 antibody [EP2952] (ab75877)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD5 antibody [EP2952] (ab75877)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD5 antibody [EP2952] (ab75877)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD5 antibody [EP2952] (ab75877)
  • OI-RD Scanning - Anti-CD5 antibody [EP2952] (ab75877)
  • Anti-CD5 antibody [EP2952] (ab75877)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP2952] to CD5
  • Suitable for: WB, IP, IHC-P
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647 Alexa Fluor® 750 Carrier Free

製品の概要

  • 製品名

    Anti-CD5 antibody [EP2952]
    CD5 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EP2952] to CD5

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IP, IHC-Pmore details
    適用なし: Flow Cyt

  • 種交差性

    交差種: Human

  • 免疫原

    Synthetic peptide within Human CD5 aa 50-150. The exact sequence is proprietary.

  • ポジティブ･コントロール

    • WB: Human lymph node lysate IHC-P: Human tonsil tissue. IP: Human lymph node lysate

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab75877の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/10000 - 1/50000. Predicted molecular weight: 55 kDa.
IP
1/50.
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
特記事項
WB
1/10000 - 1/50000. Predicted molecular weight: 55 kDa.
IP
1/50.
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
追加情報
Is unsuitable for Flow Cyt.

ターゲット情報

  • 機能

    May act as a receptor in regulating T-cell proliferation. CD5 interacts with CD72/LYB-2.

  • 配列類似性

    Contains 3 SRCR domains.

  • 細胞内局在

    Membrane.
  • Information by UniProt

  • 参照データベース

    • 別名

      • CD 5 antibody
      • CD5 antibody
      • CD5 antigen (p56 62) antibody
      • CD5 antigen antibody
      • CD5 molecule antibody
      • CD5_HUMAN antibody
      • LEU1 antibody
      • LyA antibody
      • Lymphocyte antigen T1/Leu-1 antibody
      • OTTHUMP00000236973 antibody
      • T-cell surface glycoprotein CD5 antibody
      • T1 antibody
      see all

    画像

    • Immunoprecipitation - Anti-CD5 antibody [EP2952] (ab75877)
      Immunoprecipitation - Anti-CD5 antibody [EP2952] (ab75877)

      CD5 was immunoprecipitated from 0.35 mg Human lymph node lysate 10 µg with 75877 at 1/20 dilution (0.6µg) . VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

      Lane 1: Human lymph node lysate 10 µg

      Lane 2: ab75877 IP in Human lymph node lysate

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab75877 in Human lymph node lysate

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

       

    • Western blot - Anti-CD5 antibody [EP2952] (ab75877)
      Western blot - Anti-CD5 antibody [EP2952] (ab75877)
      Anti-CD5 antibody [EP2952] (ab75877) at 1/1000 dilution (Purified) + Human lymph node lysate at 15 µg

      Secondary
      Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

      Predicted band size: 55 kDa
      Observed band size: 67 kDa why is the actual band size different from the predicted?

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD5 antibody [EP2952] (ab75877)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD5 antibody [EP2952] (ab75877)

      ab75877 showing positive staining in Normal colon lymphocytes tissue.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD5 antibody [EP2952] (ab75877)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD5 antibody [EP2952] (ab75877)

      ab75877 showing negative staining in Normal brain tissue.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD5 antibody [EP2952] (ab75877)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD5 antibody [EP2952] (ab75877)

      ab75877 showing negative staining in Normal kidney tissue.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD5 antibody [EP2952] (ab75877)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD5 antibody [EP2952] (ab75877)

      ab75877 showing negative staining in Skeletal muscle tissue.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD5 antibody [EP2952] (ab75877)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD5 antibody [EP2952] (ab75877)

      ab75877 showing positive staining in Normal spleen tissue.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • OI-RD Scanning - Anti-CD5 antibody [EP2952] (ab75877)
      OI-RD Scanning - Anti-CD5 antibody [EP2952] (ab75877)
      Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD
    • Anti-CD5 antibody [EP2952] (ab75877)
      Anti-CD5 antibody [EP2952] (ab75877)

    参考文献 (3)

    ab75877 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab75877 は 3 報の論文で使用されています。

    • Qiu C & Du G Loss of LEF-1 expression as a diagnostic indicator for extranodal NK/T-cell lymphoma: An immunohistochemical study of 88 cases. Eur J Haematol 109:513-518 (2022). PubMed: 35871391
    • Xiao LI  et al. Multiple myeloma-associated skin light chain amyloidosis: A case of misdiagnosis. Oncol Lett 11:3617-3620 (2016). IHC ; Human . PubMed: 27284363
    • Lu TX  et al. Epstein-Barr virus positive diffuse large B-cell lymphoma predict poor outcome, regardless of the age. Sci Rep 5:12168 (2015). IHC ; Human . PubMed: 26202875

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    Question

    I contact you as I would need to have the concentration of one of your antibody. This is apparently a tissue culture supernatant but I would still like to know if you could give me a range of concentration for this antibody. The reference is Ab75877.

    Read More
    Answer

    According to the datasheet, this antibody is indeed a non-purified cell culture supernatantproduct. That means, along with whole serum and Ascitesproducts, the antibody concentration cannot be determined as there are too many other proteins in this solution as well.


    However, we have a table under our Technical FAQs (Question 8, What concentration of primary antibody should I use?) which explains how much specific antibody can be expected in the non-purified products. In this case it is 1 - 3 mg/ml.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com