JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB193378

Anti-CD5 抗体 [CD5/54/F6]

Anti-CD5 antibody [CD5/54/F6]

Be the first to review this product! Submit a review

|

(2 Publications)

Mouse Monoclonal CD5 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human CD5.

別名を表示する

CD5, LEU1, T-cell surface glycoprotein CD5, Lymphocyte antigen T1/Leu-1

1 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD5 antibody [CD5/54/F6] (AB193378)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD5 antibody [CD5/54/F6] (AB193378)

Immunohistochemical analysis of formalin-fixed paraffin-embedded Human tonsil tissue labeling CD5 with ab193378 at 1 μg/ml.

Key facts

宿主種

Mouse

クローン性

Monoclonal

クローン番号

CD5/54/F6

アイソタイプ

IgG1

軽鎖のタイプ

kappa

キャリアフリー

No

交差種

Human

アプリケーション

IHC-P

applications

免疫原

Synthetic Peptide within Human CD5. The exact immunogen used to generate this antibody is proprietary information.

P06127

特異性

ab193378 is very useful in differentiating among mature small lymphoid cell malignancies. It can also be used in distinguishing thymic carcinoma (+) from thymoma (-). It does not react with granulocytes or monocytes.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "0.5-1 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." } } }
style
left-column
style
left-column

出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein G
バッファー組成
pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 0.05% BSA
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
style
left-column

補足情報

This supplementary information is collated from multiple sources and compiled automatically.

CD5 also known as T1 or Leu-1 is a glycoprotein with a molecular mass of approximately 67 kDa. It is expressed mainly on the surface of T cells and a subset of B cells. CD5 plays a major role in the regulation of immune responses. It functions by modulating T-cell receptor (TCR) signaling often acting as a negative regulator to prevent excessive immune activation. Researchers often utilize CD5 antibodies such as those conjugated with PerCP for immunohistochemistry (IHC) to study this protein's expression and distribution.
Biological function summary

CD5 is essential in the immune system's ability to maintain tolerance to self-antigens thereby preventing autoimmune responses. CD5 forms part of a receptor complex on the cell surface that interacts with ligands and other receptors to fine-tune immune cell signaling. Its localization and function in T cells relate closely to its ability to modulate signaling pathways essential for cell survival and proliferation. This modulation is important for ensuring that immune responses are appropriate to the stimuli encountered.

Pathways

CD5 is involved in signal transduction processes important for immune tolerance and modulation. It intersects with pathways like the T cell receptor (TCR) signaling pathway and the NF-kB signaling pathway. These pathways involve interaction with proteins like Zap70 a tyrosine kinase related to TCR signaling and the downstream activation of transcription factors that regulate immune responses. The protein CRIS1 also appears in some pathway interactions highlighting the interconnected nature of immune regulatory proteins.

CD5 has significant associations with autoimmune diseases such as systemic lupus erythematosus (SLE) where its modulatory role can affect autoantibody production. Additionally CD5 in conjunction with proteins like OX19 correlates with certain lymphoproliferative disorders including chronic lymphocytic leukemia (CLL). In these disorders the expression and function of CD5 may impact disease progression and patient response to therapy making it a potential target for intervention in immune-related conditions.
style
left-column
style
left-column

製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

ターゲットの情報

Lymphoid-specific receptor expressed by all T-cells and in a subset of B-cells known as B1a cells. Plays a role in the regulation of TCR and BCR signaling, thymocyte selection, T-cell effector differentiation and immune tolerance. Acts by interacting with several ligands expressed on B-cells such as CD5L or CD72 and thereby plays an important role in contact-mediated, T-dependent B-cell activation and in the maintenance of regulatory T and B-cell homeostasis. Functions as a negative regulator of TCR signaling during thymocyte development by associating with several signaling proteins including LCK, CD3Z chain, PI3K or CBL (PubMed : 1384049, PubMed : 1385158). Mechanistically, co-engagement of CD3 with CD5 enhances phosphorylated CBL recruitment leading to increased VAV1 phosphorylation and degradation (PubMed : 23376399). Modulates B-cell biology through ERK1/2 activation in a Ca(2+)-dependent pathway via the non-selective Ca(2+) channel TRPC1, leading to IL-10 production (PubMed : 27499044).
See full target information CD5
style
left-column

文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Circulation. Heart failure 16:e010294 PubMed37465947

2023

Inhibition of Pyk2 Improves Cx43 Intercalated Disc Localization, Infarct Size, and Cardiac Function in Rats With Heart Failure.

Applications

Unspecified application

Species

Unspecified reactive species

Li Zheng,Gaelle Spagnol,Devashri R Gandhi,Kanika Sharma,Vikas Kumar,Kaushik P Patel,Paul L Sorgen

Cancers 13: PubMed34638404

2021

Towards a Better Characterisation of Leukemic Cells in Chronic Lymphocytic Leukaemia: Cell-Size Heterogeneity Reflects Their Activation Status and Migratory Abilities.

Applications

Unspecified application

Species

Unspecified reactive species

Gayane Manukyan,Zuzana Mikulkova,Peter Turcsanyi,Jakub Savara,Markéta Trajerová,Zuzana Kubova,Tomas Papajik,Eva Kriegova
View all publications
style
left-column
style
left-column
style
right-column

代替製品

Primary Antibodies

AB213003

Anti-CD5 antibody [CD5/54/F6] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD5 antibody [CD5/54/F6] - BSA and Azide free (AB213003)

  • 0
  • 0
Primary Antibodies

AB213004

Anti-CD5 antibody [C5/473 + CD5/54/F6] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD5 antibody [C5/473 + CD5/54/F6] - BSA and Azide free (AB213004)

  • 0
  • 0
Primary Antibodies

AB200697

Anti-CD5 antibody [C5/473 + CD5/54/F6]

  • 0
  • 0
Primary Antibodies

AB269293

PE Anti-CD5 antibody [L17F12]

Flow Cytometry - PE Anti-CD5 antibody [L17F12] (AB269293)

  • 0
  • 0
Primary Antibodies

AB269292

PE Anti-CD5 antibody [CRIS1]

Flow Cytometry - PE Anti-CD5 antibody [CRIS1] (AB269292)

  • 0
  • 0
Primary Antibodies

AB16699

Anti-CD5 antibody [SP19]

BOND RX™ Validated|RabMAb|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD5 antibody [SP19] (AB16699)

  • 0
  • 0
Primary Antibodies

AB134174

Anti-CD5 antibody [EPR2953]

RabMAb|Recombinant

Western blot - Anti-CD5 antibody [EPR2953] (AB134174)

  • 0
  • 0
Primary Antibodies

AB75877

Anti-CD5 antibody [EP2952]

RabMAb|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD5 antibody [EP2952] (AB75877)

  • 0
  • 0
Primary Antibodies

AB194821

Anti-CD5 antibody [EP2952] - BSA and Azide free

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD5 antibody [EP2952] - BSA and Azide free (AB194821)

  • 0
  • 0
Primary Antibodies

AB239796

Anti-CD5 antibody [SP19] - BSA and Azide free

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD5 antibody [SP19] - BSA and Azide free (AB239796)

  • 4
  • 1
Primary Antibodies

AB155375

PE/Cy7® Anti-CD5 antibody [UCHT2]

Flow Cytometry - PE/Cy7® Anti-CD5 antibody [UCHT2] (AB155375)

  • 0
  • 0

組合せ製品

Assay Kits

AB314852

Human CD5 ELISA Kit - Intracellular

SimpleStep

Sandwich ELISA - Human CD5 ELISA Kit - Intracellular (AB314852)

  • 0
  • 0
Primary Antibodies

AB64088

Anti-CD20 antibody [SP32]

BOND RX™ Validated|20ul selling size|RabMAb|Advanced Validation|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD20 antibody [SP32] (AB64088)

  • 5
  • 12
Primary Antibodies

AB10558

Anti-CD45 antibody

BOND RX™ Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD45 antibody (AB10558)

  • 4
  • 61
Primary Antibodies

AB16698

Anti-CD79a antibody [SP18]

RabMAb

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD79a antibody [SP18] (AB16698)

  • 1
  • 1
Primary Antibodies

AB133616

Anti-CD4 antibody [EPR6855]

BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size|Advanced Validation

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD4 antibody [EPR6855] (AB133616)

  • 5
  • 19

Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。
保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com