Anti-CD48 抗体 [EPR26531-77] (ab302708)
![Western blot - Anti-CD48 antibody [EPR26531-77] (AB302708)](https://www.abcam.co.jp/ps/products/302/ab302708/Images/AB302708-489546-anti-cd48-antibody-epr2653177-western-blot-ramos.jpg "Western blot - Anti-CD48 antibody [EPR26531-77] (AB302708)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR26531-77] to CD48
- Suitable for: WB, ICC/IF, Flow Cyt
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-CD48 antibody [EPR26531-77]
CD48 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR26531-77] to CD48 -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IF, Flow Cytmore details
適用なし: IHC-P or IP -
種交差性
交差種: Human
非交差種: Mouse, Rat -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- WB: Whole cell lysates: Ramos (human Burkitt's lymphoma B lymphocyte), Daudi (human Burkitt's lymphoma lymphoblast), Raji (human Burkitt's lymphoma B lymphocyte); Tissue lysates: Human lymph node and tonsil. ICC/IF: Raji (human Burkitt's lymphoma B lymphocyte) Flow cyt.: Raji (human Burkitt's lymphoma B lymphocyte, human peripheral blood mononuclear cell (PBMC).
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特記事項
ab302708 does not react in: WB, immunocytochemistry-immunofluorescence and flow cytometry with mouse; IHC-P with human, mouse, and rat species; immunoprecipitation with human.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR26531-77 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab302708の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000. Detects a band of approximately 45 kDa (predicted molecular weight: 28 kDa).
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| ICC/IF |
1/100.
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| Flow Cyt |
1/500.
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| 特記事項 |
|---|
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WB
1/1000. Detects a band of approximately 45 kDa (predicted molecular weight: 28 kDa). |
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ICC/IF
1/100. |
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Flow Cyt
1/500. |
ターゲット情報
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機能
Ligand for CD2. Might facilitate interaction between activated lymphocytes. Probably involved in regulating T-cell activation. -
配列類似性
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
細胞内局在
Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 962 Human
- Omim: 109530 Human
- SwissProt: P09326 Human
- Unigene: 243564 Human
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別名
- Antigen CD48 antibody
- B cell membrane protein antibody
- B lymphocyte activation marker BLAST 1 antibody
see all
画像
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Western blot - Anti-CD48 antibody [EPR26531-77] (AB302708)All lanes : Anti-CD48 antibody [EPR26531-77] (ab302708) at 1/1000 dilution
Lane 1 : Ramos (human Burkitt's lymphoma B lymphocyte), whole cell lysate
Lane 2 : Daudi (human Burkitt's lymphoma lymphoblast), whole cell lysate
Lane 3 : Raji (human Burkitt's lymphoma B lymphocyte), whole cell lysate
Lane 4 : K-562 (human chronic myelogenous leukemia lymphoblast), whole cell lysate
Lane 5 : HeLa (human cervical adenocarcinoma epithelial cell), whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Performed under non-reducing conditions.
Predicted band size: 28 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?Blocking and dilution buffer and concentration: 5% NFDM/TBST.
The molecular weight observed is consistent with what has been described in the literature (PMID: 24626474).
Negative controls: K-562, HeLa (PMID: 26878112, 9041467)
Samples are non-boiled as boiling may cause protein aggregates.
Exposure time Lane 2: 70 seconds, Lane 1, 3-5: 26 seconds
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![Western blot - Anti-CD48 antibody [EPR26531-77] (AB302708)](https://www.abcam.co.jp/ps/products/302/ab302708/Images/AB302708-489545-anti-cd48-antibody-epr2653177-western-blot-human-lymph-node.jpg)
Western blot - Anti-CD48 antibody [EPR26531-77] (AB302708)All lanes : Anti-CD48 antibody [EPR26531-77] (ab302708) at 1/1000 dilution
Lane 1 : Human lymph node tissue lysate
Lane 2 : Human tonsil tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Performed under non-reducing conditions.
Predicted band size: 28 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?
Exposure time: 10 secondsBlocking and dilution buffer and concentration: 5% NFDM/TBST.
Samples are non-boiled as boiling may cause protein aggregates.
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![Immunocytochemistry/ Immunofluorescence - Anti-CD48 antibody [EPR26531-77] (AB302708)](https://www.abcam.co.jp/ps/products/302/ab302708/Images/AB302708-4-anti-cd48-antibody-epr2653177-immunocytochemistry-raji.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CD48 antibody [EPR26531-77] (AB302708)Immunofluorescent analysis of 4% paraformaldehyde fixed, 0.1% TritonX-100 permeabilized Raji (human Burkitt's lymphoma B lymphocyte) cells labeling CD48 with A302708 at 1/100 dilution (5.17 μg/mL), followed by preadsobed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (2 μg/mL) (Green). Confocal image showing membranous and cytoplasmic staining in Raji cell line. Negative control: no staining was observed in HeLa cell line (PMID: 9041467).
Anti-alpha Tubulin (ab195889) mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (2.5 μg/mL) (Red). The Nuclear counterstain was (Blue).
Secondary antibody only control: Primary diluent was used instead of primary antibody, followed by preadsorbed ready to use ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (2 μgmL).
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![Flow Cytometry - Anti-CD48 antibody [EPR26531-77] (AB302708)](https://www.abcam.co.jp/ps/products/302/ab302708/Images/AB302708-2-anti-cd48-antibody-epr2653177-flow-cytometry-hela.jpg)
Flow Cytometry - Anti-CD48 antibody [EPR26531-77] (AB302708)Flow cytometric analysis of HeLa (human cervix adenocarcinoma epithelial cell, Left) / Raji (human Burkitt's lymphoma B lymphocyte, Right) cells labeling CD48 with ab302708 at 1/500 dilution (0.1 µg) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti-Rabbit IgG (Alexa Fluor 488, ab150081) at 1/2000 dilution was used as the secondary antibody. Gated on viable cells. Negative control: HeLa (PMID: 9041467).
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![Flow Cytometry - Anti-CD48 antibody [EPR26531-77] (AB302708)](https://www.abcam.co.jp/ps/products/302/ab302708/Images/AB302708-3-anti-cd48-antibody-epr2653177-flow-cytometry-human-peripheral-blood-mononuclear-cell.jpg)
Flow Cytometry - Anti-CD48 antibody [EPR26531-77] (AB302708)Flow cytometric analysis of human peripheral blood mononuclear cell (PBMC) cells labeling CD48 with ab302708 at 1/500 dilution (0.1 µg) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody. Gated on viable cells.
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![Anti-CD48 antibody [EPR26531-77] (AB302708)](https://www.abcam.co.jp/ps/products/302/ab302708/Images/AB302708-1-Benefits-of-Recombinant-antibodies.jpg)
Anti-CD48 antibody [EPR26531-77] (AB302708)
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (0)
ab302708 は論文での使用が確認できていません。