Anti-CD47 抗体 [RM1014] - BSA and Azide free (ab284138)
![Western blot - Anti-CD47 antibody [RM1014] - BSA and Azide free (ab284138)](https://www.abcam.co.jp/ps/products/284/ab284138/Images/ab284138-484930-CD47WB1.jpg "Western blot - Anti-CD47 antibody [RM1014] - BSA and Azide free (ab284138)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit recombinant multiclonal [RM1014] to CD47 - BSA and Azide free
- Suitable for: WB, IHC-P, Flow Cyt, ICC/IF
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-CD47 antibody [RM1014] - BSA and Azide free
CD47 一次抗体 製品一覧 -
製品の詳細
Rabbit recombinant multiclonal [RM1014] to CD47 - BSA and Azide free -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, Flow Cyt, ICC/IFmore details
適用なし: IP -
種交差性
交差種: Human -
免疫原
This product was produced with the following immunogens:
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers. -
ポジティブ･コントロール
- WB: Jurkat, HEK-293T and U-937 whole cell lysates; human ovary cancer tissue lysate. IHC-P: Human spleen, placenta, breast cancer and ovarian carcinoma tissue. ICC/IF: Jurkat cells. Flow cyt: U-937 and Jurkat cells.
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特記事項
ab284138 is a carrier-free version of ab284132.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant multiclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 7.2
Constituent: 100% PBS -
キャリア・フリー
はい -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
Recombinant Multiclonal -
クローン名
RM1014 -
アイソタイプ
IgG -
研究分野
関連製品
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Isotype control
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KO cell lines
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab284138の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use at an assay dependent concentration. Detects a band of approximately 47-52 kDa (predicted molecular weight: 35 kDa).
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| IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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| Flow Cyt |
Use at an assay dependent concentration.
|
|
| ICC/IF |
Use at an assay dependent concentration.
|
| 特記事項 |
|---|
|
WB
Use at an assay dependent concentration. Detects a band of approximately 47-52 kDa (predicted molecular weight: 35 kDa). |
|
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
|
Flow Cyt
Use at an assay dependent concentration. |
|
ICC/IF
Use at an assay dependent concentration. |
ターゲット情報
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機能
Has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. Plays an important role in memory formation and synaptic plasticity in the hippocampus (By similarity). Receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation. May play a role in membrane transport and/or integrin dependent signal transduction. May prevent premature elimination of red blood cells. May be involved in membrane permeability changes induced following virus infection. -
組織特異性
Very broadly distributed on normal adult tissues, as well as ovarian tumors, being especially abundant in some epithelia and the brain. -
配列類似性
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
細胞内局在
Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 961 Human
- Omim: 601028 Human
- SwissProt: Q08722 Human
- Unigene: 446414 Human
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別名
- Antigen identified by monoclonal antibody 1D8 antibody
- Antigenic surface determinant protein OA3 antibody
- CD 47 antibody
see all
画像
-
Western blot - Anti-CD47 antibody [RM1014] - BSA and Azide free (ab284138)All lanes : Anti-CD47 antibody [RM1014] (ab284132) at 1/1000 dilution
Lane 1 : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate 20ug
Lane 2 : U-937 (Human histiocytic lymphoma monocyte) whole cell lysate 20ug
Lane 3 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate 20ug
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 35 kDa
Observed band size: 42-52 kDa why is the actual band size different from the predicted?
Exposure time: 37 secondsThis data was developed using ab284132, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration was 5% NFDM/TBST.
Low expression control: HepG2 (PMID: 25721088, 30415063).
We recommend that samples are not boiled after adding loading buffer as this may cause protein aggregates.
The molecular mass observed is consistent with the literature (PMID 12393467, PMID 11034562).
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![Western blot - Anti-CD47 antibody [RM1014] - BSA and Azide free (ab284138)](https://www.abcam.co.jp/ps/products/284/ab284138/Images/ab284138-484932-CD47WB2.jpg)
Western blot - Anti-CD47 antibody [RM1014] - BSA and Azide free (ab284138)All lanes : Anti-CD47 antibody [RM1014] (ab284132) at 1/1000 dilution
Lane 1 : Human liver lysate
Lane 2 : Human ovary cancer lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 35 kDa
Observed band size: 47-52 kDa why is the actual band size different from the predicted?
Exposure time: 37 secondsThis data was developed using ab284132, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration was 5% NFDM/TBST.
We recommend that samples are not boiled after adding loading buffer as this may cause protein aggregates.
-
![Western blot - Anti-CD47 antibody [RM1014] - BSA and Azide free (ab284138)](https://www.abcam.co.jp/ps/products/284/ab284138/Images/ab284138-484934-CD47KO293T.jpg)
Western blot - Anti-CD47 antibody [RM1014] - BSA and Azide free (ab284138)All lanes : Anti-CD47 antibody [RM1014] (ab284132) at 1/1000 dilution
Lane 1 : Wild-type HEK-293T (Human embryonic kidney epithelial cell, ab255449) whole cell lysate
Lane 2 : CD47 knockout HEK-293T (ab266324) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 35 kDa
Observed band size: 47-52 kDa why is the actual band size different from the predicted?
Exposure time: 37 secondsThis data was developed using ab284132, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration was 5% NFDM/TBST.
We recommend that samples are not boiled after adding loading buffer as this may cause protein aggregates.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD47 antibody [RM1014] - BSA and Azide free (ab284138)](https://www.abcam.co.jp/ps/products/284/ab284138/Images/ab284138-484938-humanspleen500.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD47 antibody [RM1014] - BSA and Azide free (ab284138)This data was developed using ab284132, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human spleen tissue labelling CD47 with ab284132 at 1/500 followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). Positive staining on human spleen. The section was incubated with ab284132 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD47 antibody [RM1014] - BSA and Azide free (ab284138)](https://www.abcam.co.jp/ps/products/284/ab284138/Images/ab284138-484939-humanplacenta500.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD47 antibody [RM1014] - BSA and Azide free (ab284138)This data was developed using ab284132, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human placenta tissue labelling CD47 with ab284132 at 1/500 followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). Positive staining on human placenta. The section was incubated with ab284132 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD47 antibody [RM1014] - BSA and Azide free (ab284138)](https://www.abcam.co.jp/ps/products/284/ab284138/Images/ab284138-484940-humanbreastcancer500.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD47 antibody [RM1014] - BSA and Azide free (ab284138)This data was developed using ab284132, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue labelling CD47 with ab284132 at 1/500 followed by a ready to use LeicaDS9800 (BOND™Polymer Refine Detection). Positive staining on human breast cancer (PMID: 31528120). The section was incubated with ab284132 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD47 antibody [RM1014] - BSA and Azide free (ab284138)](https://www.abcam.co.jp/ps/products/284/ab284138/Images/ab284138-484942-humanovariancancer500.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD47 antibody [RM1014] - BSA and Azide free (ab284138)This data was developed using ab284132, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human spleen tissue labelling CD47 with ab284132 at 1/500 followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). Positive staining on human spleen. The section was incubated with ab284132 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD47 antibody [RM1014] - BSA and Azide free (ab284138)](https://www.abcam.co.jp/ps/products/284/ab284138/Images/ab284138-484943-humanovariancancer1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD47 antibody [RM1014] - BSA and Azide free (ab284138)This data was developed using ab284132, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human spleen tissue labelling CD47 with ab284132 at 1/500 followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). Positive staining on human spleen. The section was incubated with ab284132 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
-
![Immunocytochemistry/ Immunofluorescence - Anti-CD47 antibody [RM1014] - BSA and Azide free (ab284138)](https://www.abcam.co.jp/ps/products/284/ab284138/Images/ab284138-484944-jkandhepg2.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CD47 antibody [RM1014] - BSA and Azide free (ab284138)This data was developed using ab284132, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 100% methanol-fixed, 0.1% Triton X-100 permeabilized Jurkat cells labelling CD47 with ab284132 at 1/50 dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green). Confocal image showing membranous staining in Jurkat cell line. Negative control: HepG2 (PMID: 25721088). ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.
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![Flow Cytometry - Anti-CD47 antibody [RM1014] - BSA and Azide free (ab284138)](https://www.abcam.co.jp/ps/products/284/ab284138/Images/ab284138-484945-Oligo14FC.jpg)
Flow Cytometry - Anti-CD47 antibody [RM1014] - BSA and Azide free (ab284138)This data was developed using ab284132, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of HepG2 (Human hepatocellular carcinoma epithelial cell, Left) / U-937 (Human histiocytic lymphoma monocyte, Right) cells labelling CD47 with ab284132 at 1/500 dilution (0.1µg) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody. Low expression control: HepG2 (PMID: 25721088, 30415063). Gated on viable cells.
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![Flow Cytometry - Anti-CD47 antibody [RM1014] - BSA and Azide free (ab284138)](https://www.abcam.co.jp/ps/products/284/ab284138/Images/ab284138-484946-Oligo14Jurkat.jpg)
Flow Cytometry - Anti-CD47 antibody [RM1014] - BSA and Azide free (ab284138)This data was developed using ab284132, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of HepG2 (Human hepatocellular carcinoma epithelial cell, Left) / Jurkat (Human T cell leukemia T lymphocyte, Right) cells labelling CD47 with ab284132 at 1/500 dilution (0.1µg) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody. Low expression control: HepG2 (PMID: 25721088, 30415063). Gated on viable cells.
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![Anti-CD47 antibody [RM1014] - BSA and Azide free (ab284138)](https://www.abcam.co.jp/ps/products/284/ab284138/Images/ab284138-484860-Benefits-of-Recombinant-antibodies.jpg)
Anti-CD47 antibody [RM1014] - BSA and Azide free (ab284138)
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (0)
ab284138 は論文での使用が確認できていません。
