Anti-CD45RO 抗体 [UCH-L1] (ab23)
Key features and details
- Mouse monoclonal [UCH-L1] to CD45RO
- Suitable for: WB, IHC-P, Flow Cyt
- Reacts with: Human
- Isotype: IgG2a
製品の概要
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製品名
Anti-CD45RO antibody [UCH-L1]
CD45RO 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [UCH-L1] to CD45RO -
由来種
Mouse -
アプリケーション
適用あり: WB, IHC-P, Flow Cytmore details -
種交差性
交差種: Human -
免疫原
Tissue, cells or virus corresponding to CD45RO. Cultured T cells from an IL-2-dependent T-cell line (CA1) prepared from human peripheral blood activated with influenza virus.
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ポジティブ・コントロール
- IHC-P: Human tonsil tissue. WB: Human tonsil and thymus tissue lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.02% Sodium azide
Constituent: 99.98% PBS -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
UCH-L1 -
ミエローマ
P3-NS1/1-Ag4-1 -
アイソタイプ
IgG2a -
研究分野
関連製品
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Compatible Secondaries
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Conjugation kits
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab23の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use a concentration of 5 µg/ml. Detects a band of approximately 180 kDa (predicted molecular weight: 131 kDa).
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IHC-P | (3) |
Use at an assay dependent concentration.
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Flow Cyt |
Use at an assay dependent concentration.
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特記事項 |
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WB
Use a concentration of 5 µg/ml. Detects a band of approximately 180 kDa (predicted molecular weight: 131 kDa). |
IHC-P
Use at an assay dependent concentration. |
Flow Cyt
Use at an assay dependent concentration. |
ターゲット情報
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機能
Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity. -
関連疾患
Defects in PTPRC are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)B(+)NK(+) SCID) [MIM:608971]. A form of severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients present in infancy recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development.
Genetic variations in PTPRC are involved in multiple sclerosis susceptibility (MS) [MIM:126200]. MS is a neurodegenerative disorder characterized by the gradual accumulation of focal plaques of demyelination particularly in the periventricular areas of the brain. Peripheral nerves are not affected. Onset usually in third or fourth decade with intermittent progression over an extended period. The cause is still uncertain. -
配列類似性
Belongs to the protein-tyrosine phosphatase family. Receptor class 1/6 subfamily.
Contains 2 fibronectin type-III domains.
Contains 2 tyrosine-protein phosphatase domains. -
ドメイン
The first PTPase domain interacts with SKAP1. -
翻訳後修飾
Heavily N- and O-glycosylated. -
細胞内局在
Membrane. Membrane raft. Colocalized with DPP4 in membrane rafts. - Information by UniProt
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参照データベース
- Entrez Gene: 5788 Human
- Omim: 151460 Human
- SwissProt: P08575 Human
- Unigene: 654514 Human
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別名
- B220 antibody
- CD45 antibody
- cd45 antigen antibody
see all
画像
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ab23 (1 µg/ml) staining CD45RO in human tonsil.
Sections were stained using an automated system (DAKO Autostainer Plus ), at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate pH 6.1. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with hematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required. -
All lanes : Anti-CD45RO antibody [UCH-L1] (ab23) at 5 µg/ml
Lane 1 : Human tonsil normal tissue lysate - total protein (ab29615)
Lane 2 : Human thymus tissue lysate - total protein (ab30146)
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 131 kDa
Observed band size: 180 kDa why is the actual band size different from the predicted?
Additional bands at: 120 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 8 minutes
CD45RO contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (22)
ab23 は 22 報の論文で使用されています。
- Rafat M et al. Bioengineered corneal tissue for minimally invasive vision restoration in advanced keratoconus in two clinical cohorts. Nat Biotechnol 41:70-81 (2023). PubMed: 35953672
- Yu J et al. Pandemic influenza A (H1N1) virus causes abortive infection of primary human T cells. Emerg Microbes Infect 11:1191-1204 (2022). PubMed: 35317717
- Yang X et al. Integrative immunogenomic analysis reveals transcriptional and immune-related differences in hepatocellular carcinoma patients with different disease-free survival. Am J Cancer Res 12:1752-1765 (2022). PubMed: 35530269
- Wu K et al. Accumulation of CD45RO+CD8+ T cells is a diagnostic and prognostic biomarker for clear cell renal cell carcinoma. Aging (Albany NY) 13:14304-14321 (2021). PubMed: 34016791
- Sudo T et al. Diversity and shared T-cell receptor repertoire analysis in esophageal squamous cell carcinoma. Oncol Lett 22:618 (2021). PubMed: 34257726