JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB30446

Anti-CD45 抗体 [YTH24.5]

Anti-CD45 antibody [YTH24.5]

Be the first to review this product! Submit a review

|

(8 Publications)

Rat Monoclonal CD45 antibody. Suitable for Flow Cyt and reacts with Human samples. Cited in 8 publications. Immunogen corresponding to Cell preparation containing PTPRC protein.

別名を表示する

CD45, PTPRC, Receptor-type tyrosine-protein phosphatase C, Leukocyte common antigen, T200, L-CA

1 Images
Flow Cytometry - Anti-CD45 antibody [YTH24.5] (AB30446)
  • Flow Cyt

Unknown

Flow Cytometry - Anti-CD45 antibody [YTH24.5] (AB30446)

Overlay histogram showing peripheral blood lymphocytes stained with ab30446 (red line). The cells were incubated with the antibody (ab30446, 1μg/1x106 cells) for 30 min at 4°C. The secondary antibody used was DyLight® 488 goat anti-rat IgG (Fc) (ab96971) at 1/250 dilution for 30 min at 22°C. Isotype control antibody (black line) was rat IgG2b [RTK4530] (ab18541, 2μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed gating on peripheral blood lymphocytes.

Key facts

宿主種

Rat

クローン性

Monoclonal

クローン番号

YTH24.5

アイソタイプ

IgG2b

キャリアフリー

No

交差種

Human

アプリケーション

Flow Cyt

applications

免疫原

Cell preparation containing PTPRC protein. The exact immunogen used to generate this antibody is proprietary information.

P08575

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1 µg for 10^6 Cells", "FlowCyt-species-notes": "<p><a href='/products/unavailable/rat-igg2b-kappa-monoclonal-rtk4530-isotype-control-bsa-and-azide-free-ab18536'>ab18536</a> - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.</p>" } } }
style
left-column
style
left-column

出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
精製に関する特記事項
Immunoglobulin fraction prepared by ammonium sulphate precipitation.
バッファー組成
Preservative: 0.09% Sodium azide Constituents: PBS
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
style
left-column

補足情報

This supplementary information is collated from multiple sources and compiled automatically.

CD45 also known as Protein tyrosine phosphatase receptor type C (PTPRC) is a transmembrane glycoprotein with a molecular mass ranging between 180-240 kDa depending on its isoform. It is expressed on the surface of almost all hematopoietic cells except for mature erythrocytes and platelets. CD45 has a critical role in regulating antigen receptor signaling by modifying kinases involved in signal transduction and it is essential in lymphocyte development and activation. Because of its broad expression on immune cells CD45 is a valuable marker for differentiating various immune cell types in assays like flow cytometry and immunohistochemistry (IHC) often referred to as CD45 stain.
Biological function summary

CD45 acts by regulating tyrosine phosphorylation in the immune cell signaling context participating directly in signal transduction. It functions by dephosphorylating specific phosphotyrosine residues on various proteins modulating the signaling threshold required for lymphocyte activation. Although not known to be part of a protein complex CD45 itself shows isoform variation that associates with specific immune cell types impacting their function. Importantly CD45 interacts with multiple signaling molecules to affect cell growth and differentiation.

Pathways

Scientists associate CD45 with signaling pathways such as the T-cell receptor (TCR) and B-cell receptor (BCR) signaling. CD45 modulates the activity of Src family kinases important elements in these pathways making it essential for effective immune response and tolerance. Furthermore interactions between CD45 and proteins like Lck in T-cells or Lyn in B-cells highlight its pivotal role in executing its signaling functions.

Scientists often link CD45 to immune-related conditions including autoimmunity and leukemia. In autoimmune diseases altered CD45 expression or activity can disrupt normal immune function contributing to pathogenesis. In leukemia CD45 expression levels can assist in disease classification and prognosis as it interacts with proteins involved in cell cycle regulation. The anti-CD45 antibodies can provide diagnostic and therapeutic avenues highlighting their utility in disease management.
style
left-column
style
left-column

製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

ターゲットの情報

Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor (PubMed : 35767951). Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity (By similarity). Interacts with CLEC10A at antigen presenting cell-T cell contact; CLEC10A on immature dendritic cells recognizes Tn antigen-carrying PTPRC/CD45 receptor on effector T cells and modulates T cell activation threshold to limit autoreactivity.. (Microbial infection) Acts as a receptor for human cytomegalovirus protein UL11 and mediates binding of UL11 to T-cells, leading to reduced induction of tyrosine phosphorylation of multiple signaling proteins upon T-cell receptor stimulation and impaired T-cell proliferation.
See full target information PTPRC
style
left-column

文献 (8)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:29306 PubMed40790149

2025

Target sequence of single cells captured by a polymeric microfluidic device.

Applications

Unspecified application

Species

Unspecified reactive species

Rintaro Oyama,Masataka Mori,Hiroki Matsumiya,Yukiko Nemoto,Natsumasa Nishizawa,Yohei Honda,Masatoshi Kanayama,Akihiro Taira,Taiji Kuwata,Masaru Takenaka,Koji Kuroda,Kazue Yoneda,Takashi Ohnaga,Motoyoshi Endo,Fumihiro Tanaka

Stem cells international 2019:5310202 PubMed31885614

2019

The Effect of Vascular Endothelial Growth Factor on Bone Marrow Mesenchymal Stem Cell Engraftment in Rat Fibrotic Liver upon Transplantation.

Applications

Unspecified application

Species

Unspecified reactive species

Ke Yuan,Chunyou Lai,Lingling Wei,Tianhang Feng,Qinyan Yang,Tianying Zhang,Tao Lan,Yutong Yao,Guangming Xiang,Xiaolun Huang

Stem cell reviews and reports 16:181-185 PubMed31758373

2019

Identification of Human Very Small Embryonic like Stem Cells (VSELS) in Human Heart Tissue Among Young and Old Individuals.

Applications

Unspecified application

Species

Unspecified reactive species

Mohamed El-Helw,Lakshman Chelvarajan,Mohamed Abo-Aly,Mohanad Soliman,Greg Milburn,Autumn L Conger,Kenneth Campbell,Mariusz Z Ratajczak,Ahmed Abdel-Latif

Oncology letters 18:6397-6404 PubMed31807163

2019

Detection of circulating colorectal cancer cells by a custom microfluid system before and after endoscopic metallic stent placement.

Applications

Unspecified application

Species

Unspecified reactive species

Rei Ishibashi,Shuntaro Yoshida,Nariaki Odawara,Takahiro Kishikawa,Ryo Kondo,Ayako Nakada,Ryunosuke Hakuta,Naminatsu Takahara,Eri Tanaka,Kazuma Sekiba,Takahiro Seimiya,Takashi Ohnaga,Motoyuki Otsuka,Kazuhiko Koike

Stem cell research & therapy 10:225 PubMed31358049

2019

Exosomes derived from mesenchymal stem cells reverse EMT via TGF-β1/Smad pathway and promote repair of damaged endometrium.

Applications

Unspecified application

Species

Unspecified reactive species

Yuan Yao,Ran Chen,Guowu Wang,Yu Zhang,Fang Liu

The Journal of experimental medicine 211:1925-35 PubMed25180064

2014

Niche displacement of human leukemic stem cells uniquely allows their competitive replacement with healthy HSPCs.

Applications

Unspecified application

Species

Unspecified reactive species

Allison L Boyd,Clinton J V Campbell,Claudia I Hopkins,Aline Fiebig-Comyn,Jennifer Russell,Jelena Ulemek,Ronan Foley,Brian Leber,Anargyros Xenocostas,Tony J Collins,Mickie Bhatia

Proceedings of the National Academy of Sciences of 110:E4978-86 PubMed24297935

2013

Ascites analysis by a microfluidic chip allows tumor-cell profiling.

Applications

Unspecified application

Species

Human

Vanessa M Peterson,Cesar M Castro,Jaehoon Chung,Nathan C Miller,Adeeti V Ullal,Maria D Castano,Richard T Penson,Hakho Lee,Michael J Birrer,Ralph Weissleder

Cancer 80:2425-30 PubMed9406693

1997

Processing of antibodies bound to B-cell lymphomas and lymphoblastoid cell lines.

Applications

Unspecified application

Species

Unspecified reactive species

N Vangeepuram,G L Ong,M J Mattes
View all publications
style
left-column
style
left-column
style
right-column

代替製品

Primary Antibodies

AB200315

Alexa Fluor® 488 Anti-CD45 antibody [EP322Y]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-CD45 antibody [EP322Y] (AB200315)

  • 0
  • 0
Primary Antibodies

AB221245

APC Anti-CD45 antibody [EP322Y]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - APC Anti-CD45 antibody [EP322Y] (AB221245)

  • 0
  • 0
Primary Antibodies

AB40763

Anti-CD45 antibody [EP322Y]

20ul selling size|RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-CD45 antibody [EP322Y] (AB40763)

  • 5
  • 14
Primary Antibodies

AB208022

Anti-CD45 antibody [EPR20033]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD45 antibody [EPR20033] (AB208022)

  • 5
  • 2
Primary Antibodies

AB229292

Anti-CD45 antibody [EPR20033] - BSA and Azide free

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD45 antibody [EPR20033] - BSA and Azide free (AB229292)

  • 5
  • 2
Primary Antibodies

AB214437

Anti-CD45 antibody [EP322Y] - BSA and Azide free

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD45 antibody [EP322Y] - BSA and Azide free (AB214437)

  • 5
  • 1

組合せ製品

Assay Kits

AB277445

Mouse CD45 ELISA kit

ELISA - Mouse CD45 ELISA kit (AB277445)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB16669

Anti-CD3 epsilon antibody [SP7]

BOND RX™ Validated|RabMAb|Advanced Validation|Recombinant|Lab Essentials|20ul selling size

Multiplex immunohistochemistry - Anti-CD3 epsilon antibody [SP7] (AB16669)

  • 4
  • 79
Primary Antibodies

AB133616

Anti-CD4 antibody [EPR6855]

BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size|Advanced Validation

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD4 antibody [EPR6855] (AB133616)

  • 5
  • 20
Primary Antibodies

AB955

Anti-CD68 antibody [KP1]

Recombinant|Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [KP1] (AB955)

  • 4
  • 48

Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。
保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com