Anti-CD45 抗体 (ab154885)
Key features and details
- Rabbit polyclonal to CD45
- Suitable for: ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-CD45 antibody
CD45 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to CD45 -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IFmore details -
種交差性
交差種: Mouse, Human -
免疫原
Synthetic peptide, corresponding to a region within amino acids 914-1004 of Human CD45 (UniProt ID: P08575).
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ポジティブ･コントロール
- 293T and A431 whole cell lysate. Mouse ESC D3 cells.
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特記事項
This antibody provides variable results in WB.
Keep as concentrated solution.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab154885の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF |
1/100 - 1/1000.
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| 特記事項 |
|---|
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ICC/IF
1/100 - 1/1000. |
ターゲット情報
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機能
Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. -
関連疾患
Defects in PTPRC are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)B(+)NK(+) SCID) [MIM:608971]. A form of severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients present in infancy recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development.
Genetic variations in PTPRC are involved in multiple sclerosis susceptibility (MS) [MIM:126200]. MS is a neurodegenerative disorder characterized by the gradual accumulation of focal plaques of demyelination particularly in the periventricular areas of the brain. Peripheral nerves are not affected. Onset usually in third or fourth decade with intermittent progression over an extended period. The cause is still uncertain. -
配列類似性
Belongs to the protein-tyrosine phosphatase family. Receptor class 1/6 subfamily.
Contains 2 fibronectin type-III domains.
Contains 2 tyrosine-protein phosphatase domains. -
ドメイン
The first PTPase domain interacts with SKAP1. -
翻訳後修飾
Heavily N- and O-glycosylated. -
細胞内局在
Membrane. Membrane raft. Colocalized with DPP4 in membrane rafts. - Information by UniProt
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参照データベース
- Entrez Gene: 5788 Human
- Entrez Gene: 19264 Mouse
- Omim: 151460 Human
- SwissProt: P08575 Human
- SwissProt: P06800 Mouse
- Unigene: 654514 Human
- Unigene: 391573 Mouse
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別名
- B220 antibody
- CD 45 antibody
- CD45 antibody
see all
画像
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Immunocytochemistry/ Immunofluorescence - Anti-CD45 antibody (ab154885)Immunofluorescence analysis of paraformaldehyde fixed Mouse ESC D3 cells, labeling CD45 using ab154885 at 1/200 dilution. Lower image costained with Hoechst 33342.
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Immunocytochemistry/ Immunofluorescence - Anti-CD45 antibody (ab154885)Immunofluorescence analysis of THP-1 cells labeling CD45 with ab154885 at 1/500 dilution. Hoechst 33342 (blue) staining is used (right panel).
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab154885 は 4 報の論文で使用されています。
- Heger LA et al. Inhibition of protein arginine deiminase 4 prevents inflammation-mediated heart failure in arthritis. Life Sci Alliance 6:N/A (2023). PubMed: 37500179
- Sahoo A et al. Expansion and characterization of human limbus-derived stromal/mesenchymal stem cells in xeno-free medium for therapeutic applications. Stem Cell Res Ther 14:89 (2023). PubMed: 37061739
- Urdang ZD et al. Toll-like Receptor 4 Signaling and Downstream Neutrophilic Inflammation Mediate Endotoxemia-Enhanced Blood-Labyrinth Barrier Trafficking. Otol Neurotol 41:123-132 (2020). PubMed: 31568132
- Sawhney S et al. Alpha-enolase is upregulated on the cell surface and responds to plasminogen activation in mice expressing a ?133p53a mimic. PLoS One 10:e0116270 (2015). WB ; Mouse . PubMed: 25643152