Anti-CD44v3 抗体 [VFF-327] (ab34229)
Key features and details
- Mouse monoclonal [VFF-327] to CD44v3
- Suitable for: IHC-Fr, IHC-P, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
製品の概要
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製品名
Anti-CD44v3 antibody [VFF-327] -
製品の詳細
Mouse monoclonal [VFF-327] to CD44v3 -
由来種
Mouse -
特異性
This antibody specifically recognises an epitope encoded by exon V3 on the variant portion of human CD44 -
アプリケーション
適用あり: IHC-Fr, IHC-P, Flow Cytmore details -
種交差性
交差種: Human -
免疫原
The details of the immunogen for this antibody are not available.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
バッファー
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading... -
精製度
Protein G purified -
ポリ/モノ
モノクローナル -
クローン名
VFF-327 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Conjugation kits
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab34229の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-Fr |
Use a concentration of 10 µg/ml.
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| IHC-P |
Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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| Flow Cyt |
Use 10µl for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. Use 10µl (at a concentration of 10 µg/ml) for 106 cells in 100µl. |
| 特記事項 |
|---|
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IHC-Fr
Use a concentration of 10 µg/ml. |
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IHC-P
Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
|
Flow Cyt
Use 10µl for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. Use 10µl (at a concentration of 10 µg/ml) for 106 cells in 100µl. |
ターゲット情報
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関連性
Receptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Altered expression or dysfunction causes numerous pathogenic phenotypes. Great protein heterogeneity due to numerous alternative splicing and post-translational modification events. There are 17 different isoforms. -
細胞内局在
Membrane; single-pass type I membrane protein -
参照データベース
- Entrez Gene: 960 Human
- Omim: 107269 Human
- SwissProt: P16070 Human
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別名
- CD44 antigen antibody
- CD44 molecule antibody
- Heparan sulfate proteoglycan antibody
see all
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab34229 は 4 報の論文で使用されています。
- Yan B et al. Clinicopathological significance and prognostic implication of CD44 and its splice variants (v3 and v6) in colorectal cancer. Transl Cancer Res 9:1215-1224 (2020). PubMed: 35117466
- Li J et al. Elevated N-methyltransferase expression induced by hepatic stellate cells contributes to the metastasis of hepatocellular carcinoma via regulation of the CD44v3 isoform. Mol Oncol 13:1993-2009 (2019). PubMed: 31294922
- Alam TN et al. Differential expression of CD44 during human prostate epithelial cell differentiation. J Histochem Cytochem 52:1083-90 (2004). PubMed: 15258184
- Seiter S et al. Mitigation of delayed-type hypersensitivity reactions by a CD44 variant isoform v3-specific antibody: blockade of leukocyte egress. J Invest Dermatol 113:11-21 (1999). PubMed: 10417612