Anti-CD43 抗体 [W3/13] (ab22351)
Key features and details
- Mouse monoclonal [W3/13] to CD43
- Suitable for: IHC-Fr, IHC-P, Flow Cyt
- Reacts with: Rat
- Isotype: IgG1
Related conjugates and formulations
製品の概要
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製品名
Anti-CD43 antibody [W3/13]
CD43 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [W3/13] to CD43 -
由来種
Mouse -
アプリケーション
適用あり: IHC-Fr, IHC-P, Flow Cytmore details -
種交差性
交差種: Rat -
免疫原
Tissue, cells or virus. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- Flow Cyt: Lewis rat splenocytes. IHC-Fr: Rat spleen tissue. IHC-P: Rat spleen tissue.
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特記事項
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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精製度
Protein G purified -
特記事項(精製)
Purified from TCS. -
ポリ/モノ
モノクローナル -
クローン名
W3/13 -
アイソタイプ
IgG1 -
軽鎖の種類
kappa -
研究分野
関連製品
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Alternative Versions
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab22351の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-Fr |
Use a concentration of 1 µg/ml.
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IHC-P |
Use a concentration of 1 µg/ml.
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Flow Cyt |
Use a concentration of 0.2 µg/ml.
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特記事項 |
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IHC-Fr
Use a concentration of 1 µg/ml. |
IHC-P
Use a concentration of 1 µg/ml. |
Flow Cyt
Use a concentration of 0.2 µg/ml. |
ターゲット情報
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機能
One of the major glycoproteins of thymocytes and T lymphocytes. Plays a role in the physicochemical properties of the T-cell surface and in lectin binding. Presents carbohydrate ligands to selectins. Has an extended rodlike structure that could protrude above the glycocalyx of the cell and allow multiple glycan chains to be accessible for binding. Is a counter receptor for SN/Siglec-1 (By similarity). During T-cell activation is actively removed from the T-cell-APC (antigen-presenting cell) contact site thus suggesting a negative regulatory role in adaptive immune response. -
組織特異性
Cell surface of thymocytes, T-lymphocytes, neutrophils, plasma cells and myelomas. -
翻訳後修飾
Glycosylated; has a high content of sialic acid and O-linked carbohydrate structures. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 24796 Rat
- SwissProt: P13838 Rat
- Unigene: 11144 Rat
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別名
- CD 43 antibody
- CD43 antibody
- CD43 antigen antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD43 antibody [W3/13] (ab22351)
IHC image of CD43 staining in a section of formalin-fixed paraffin-embedded normal rat spleen* performed on a Leica BOND™ system using the standard Protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab22351, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from Charles River.
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IHC image of CD43 staining in a section of frozen normal rat spleen*.
The section was fixed using 10% formaldehyde in 1XPBS for 10 minutes. No antigen retrieval step was performed prior to staining. Non-specific protein-protein interactions were then blocked in TBS containing 0.025% (v/v) Triton X-100, 0.3M glycine and 1% (w/v) BSA for 1h at room temperature. The section was then incubated overnight at +4°C in TBS containing 0.025% (v/v) Triton X-100 and 1% (w/v) BSA with ab22351 (shown in red) at 1µg/ml and co-localization of T cells stained using ab16669 (shown in green), Rabbit monoclonal to CD3, at 1/150 dilution. The section was then incubated with ab150119 (Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor®647, 1/1000)) and ab150077 (Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor®488, 1/1000)) for 1 hour at room temperature. The secondary-only control image is taken from an identical assay without primary antibody. The section was then mounted using Fluoromount®.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
For IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antibody concentrations and incubation times.
*Tissue obtained from Charles River.
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Lewis rat splenocytes stained with ab22351 (right) or mouse IgG1κ (left). Lewis rat splenocytes were incubated for 30 min on ice in 10% rat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab22351) or mouse IgG1κ Isotype (ab170190) (1x106 in 100µl at 0.2 µg/ml) for 30 min on ice.
The secondary antibody Goat Anti-Mouse IgG H&L (Alexa Fluor ® 488, pre-adsorbed) (ab150117) was used at 1/2000 dilution for 30 min at 4°C. The cells were simultaneously stained with CD3 APC antibody.
Acquisition of >30,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter. Events were gated on viable lymphocytes.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (6)
ab22351 は 6 報の論文で使用されています。
- Deprez A et al. Transient neonatal exposure to hyperoxia, an experimental model of preterm birth, leads to skeletal muscle atrophy and fiber type switching. Clin Sci (Lond) 135:2589-2605 (2021). PubMed: 34750633
- Gao F et al. Enalapril mitigates focal alveolar lesions, a histological marker of late pulmonary injury by radiation to the lung. Radiat Res 179:465-74 (2013). PubMed: 23480564
- De Miguel C et al. High dietary protein exacerbates hypertension and renal damage in Dahl SS rats by increasing infiltrating immune cells in the kidney. Hypertension 57:269-74 (2011). IHC-P ; Rat . PubMed: 21173345
- Skoumal R et al. Parthenolide inhibits STAT3 signaling and attenuates angiotensin II-induced left ventricular hypertrophy via modulation of fibroblast activity. J Mol Cell Cardiol 50:634-41 (2011). IHC-P ; Rat . PubMed: 21223972
- De Miguel C et al. Infiltrating T lymphocytes in the kidney increase oxidative stress and participate in the development of hypertension and renal disease. Am J Physiol Renal Physiol 300:F734-42 (2011). IHC-P ; Rat . PubMed: 21159736
- Moilanen AM et al. Intramyocardial BNP gene delivery improves cardiac function through distinct context-dependent mechanisms. Circ Heart Fail 4:483-95 (2011). PubMed: 21558448