Anti-CD43 抗体 [MEM-59] (ab9088)
![Flow Cytometry - Anti-CD43 antibody [MEM-59] (ab9088)](https://www.abcam.co.jp/ps/products/9/ab9088/Images/ab9088-521303-anti-cd43-antibody-mem-59-flow-cytometry-lymphocytes-human.jpg "Flow Cytometry - Anti-CD43 antibody [MEM-59] (ab9088)")
Key features and details
- Mouse monoclonal [MEM-59] to CD43
- Suitable for: Flow Cyt, IHC-P
- Reacts with: Human
- Isotype: IgG1
Related conjugates and formulations
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-CD43 antibody [MEM-59]
CD43 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [MEM-59] to CD43 -
由来種
Mouse -
特異性
Recognizes neuraminidase-sensitive epitope on human CD43 molecule (Leukosialin), which is involved in lymphocyte activation. -
アプリケーション
適用あり: Flow Cyt, IHC-Pmore details -
種交差性
交差種: Human -
免疫原
Tissue, cells or virus corresponding to Human CD43. Human T lymphocytes
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ポジティブ･コントロール
- IHC-P: Human spleen tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading... -
精製度
Protein A purified -
特記事項（精製）
Purified from TCS. Purity >95% by SDS-PAGE. -
ポリ/モノ
モノクローナル -
クローン名
MEM-59 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab9088の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Flow Cyt |
Use a concentration of 1 - 5 µg/ml.
|
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| IHC-P |
Use a concentration of 10 µg/ml.
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| 特記事項 |
|---|
|
Flow Cyt
Use a concentration of 1 - 5 µg/ml. |
|
IHC-P
Use a concentration of 10 µg/ml. |
ターゲット情報
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機能
One of the major glycoproteins of thymocytes and T lymphocytes. Plays a role in the physicochemical properties of the T-cell surface and in lectin binding. Presents carbohydrate ligands to selectins. Has an extended rodlike structure that could protrude above the glycocalyx of the cell and allow multiple glycan chains to be accessible for binding. Is a counter receptor for SN/Siglec-1 (By similarity). During T-cell activation is actively removed from the T-cell-APC (antigen-presenting cell) contact site thus suggesting a negative regulatory role in adaptive immune response. -
組織特異性
Cell surface of thymocytes, T-lymphocytes, neutrophils, plasma cells and myelomas. -
翻訳後修飾
Glycosylated; has a high content of sialic acid and O-linked carbohydrate structures. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 6693 Human
- Omim: 182160 Human
- SwissProt: P16150 Human
- Unigene: 632188 Human
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別名
- CD 43 antibody
- CD43 antibody
- CD43 antigen antibody
see all
画像
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Flow Cytometry - Anti-CD43 antibody [MEM-59] (ab9088)
Flow cytometric analysis of Human Peripheral Blood cells labelling CD43 with ab9088 at 2 ug/ml showing separation of human CD43 positive lymphocytes (red-filled) from human CD43 negative blood debris (black-dashed).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD43 antibody [MEM-59] (ab9088)](https://www.abcam.co.jp/ps/products/9/ab9088/Images/ab9088-521305-anti-cd43-antibody-mem-59-immunohistochemistry-spleen-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD43 antibody [MEM-59] (ab9088)Immunohistochemical analysis of Paraffin-embedded sections Human Spleen tissue labelling CD43 with ab9088.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (7)
ab9088 は 7 報の論文で使用されています。
- Martin-Jaular L et al. Unbiased proteomic profiling of host cell extracellular vesicle composition and dynamics upon HIV-1 infection. EMBO J 40:e105492 (2021). PubMed: 33709510
- Wisnovsky S et al. Genome-wide CRISPR screens reveal a specific ligand for the glycan-binding immune checkpoint receptor Siglec-7. Proc Natl Acad Sci U S A 118:N/A (2021). PubMed: 33495350
- Cermak L et al. Molecular mechanisms involved in CD43-mediated apoptosis of TF-1 cells. Roles of transcription Daxx expression, and adhesion molecules. J Biol Chem 277:7955-61 (2002). PubMed: 11773067
- Alvarado M et al. MEM-59 monoclonal antibody detects a CD43 epitope involved in lymphocyte activation. Eur J Immunol 25:1051-5 (1995). PubMed: 7537669
- Deckert M et al. CD59 molecule: a second ligand for CD2 in T cell adhesion. Eur J Immunol 22:2943-7 (1992). PubMed: 1385156
- Stefanova I et al. Characterization of a broadly expressed human leucocyte surface antigen MEM-43 anchored in membrane through phosphatidylinositol. Mol Immunol 26:153-61 (1989). PubMed: 2918859
- Stefanova I et al. Characterization of a 95 kDa human leucocyte sialoglycoprotein: its identity with CD43, gpL115, leukosialin and sialophorin. Folia Biol (Praha) 34:255-65 (1988). PubMed: 2467832