AB212058

Anti-CD40 抗体 [EPR18005-4]

Anti-CD40 antibody [EPR18005-4]

RabMAb
Recombinant
詳細を見る

Be the first to review this product! Submit a review

(1 Publication)

Rabbit Recombinant Monoclonal CD40 antibody. Suitable for Flow Cyt, WB, ICC/IF and reacts with Mouse samples. Cited in 1 publication.

別名を表示する

CD40, Tnfrsf5, Cd40, Tumor necrosis factor receptor superfamily member 5, B-cell surface antigen CD40, Bp50, CD40L receptor

3 Images

Flow Cytometry - Anti-CD40 antibody [EPR18005-4] (AB212058)

Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD40 antibody [EPR18005-4] (AB212058)

Flow cytometric analysis of A20 (mouse reticulum sarcoma cell line) cell line labeling CD40 with ab212058 at 1/600 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.

Immunocytochemistry/ Immunofluorescence - Anti-CD40 antibody [EPR18005-4] (AB212058)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CD40 antibody [EPR18005-4] (AB212058)

Immunofluorescent analysis of 100% methanol-fixed A20 (mouse reticulum sarcoma cell line) cells labeling CD40 with ab212058 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Membranous staining on A20 cell line.

The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) (ab195889) (red) at 1/200 dilution.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.

Supplier Data

Western blot - Anti-CD40 antibody [EPR18005-4] (AB212058)

Blocking/Dilution buffer : 5% NFDM/TBST.

The molecular mass observed is consistent with the literature (PMID : 23404288 and PMID : 16709811).

All lanes:

Western blot - Anti-CD40 antibody [EPR18005-4] (ab212058) at 1/1000 dilution

All lanes:

A20 (mouse reticulum sarcoma cell line) whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 30 kDa

Observed band size: 42 kDa

true

Exposure time: 3min

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR18005-4

アイソタイプ

IgG

キャリアフリー

交差種

Mouse

アプリケーション

ICC/IF, Flow Cyt, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

特異性

ab212058 recognises a different epitope to ab188181.

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/600", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "" } } }

style

left-column

製品の詳細

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

style

left-column

style

left-column

style

left-column

製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

style

left-column

ターゲットの情報

Receptor for TNFSF5/CD40LG (By similarity). Transduces TRAF6- and MAP3K8-mediated signals that activate ERK in macrophages and B cells, leading to induction of immunoglobulin secretion (PubMed : 12881420).

See full target information Cd40

style

left-column

文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Pflugers Archiv : European journal of physiology 476:1895-1911 PubMed39392480

2024

A role for plasma membrane Ca ATPases in regulation of cellular Ca homeostasis by sphingosine kinase-1.

Applications

Unspecified application

Species

Unspecified reactive species

Luisa Michelle Volk,Jan-Erik Bruun,Sandra Trautmann,Dominique Thomas,Stephanie Schwalm,Josef Pfeilschifter,Dagmar Meyer Zu Heringdorf

View all publications

style

left-column

style

left-column

websiteProtocolBooklet

style

right-column

Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CD40 抗体 [EPR18005-4]

Anti-CD40 antibody [EPR18005-4]

Flow Cytometry - Anti-CD40 antibody [EPR18005-4] (AB212058)

Immunocytochemistry/ Immunofluorescence - Anti-CD40 antibody [EPR18005-4] (AB212058)

Western blot - Anti-CD40 antibody [EPR18005-4] (AB212058)

関連する標識済み抗体及び組成の異なる製品 (2)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

特異性

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

製品プロトコール

ターゲットの情報

文献 (1)

代替製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

組合せ製品

Assay Kits

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Abcam product promise