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AB252557

Anti-CD40 抗体 [BLR056F]

Anti-CD40 antibody [BLR056F]

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(2 Publications)

Rabbit Recombinant Monoclonal CD40 antibody. Suitable for IHC-P, ICC, IP, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human CD40 aa 200-300.

別名を表示する

CD40, TNFRSF5, Tumor necrosis factor receptor superfamily member 5, B-cell surface antigen CD40, Bp50, CD40L receptor, CDw40

6 Images
Immunocytochemistry - Anti-CD40 antibody [BLR056F] (AB252557)
  • ICC

Supplier Data

Immunocytochemistry - Anti-CD40 antibody [BLR056F] (AB252557)

FFPE section of Raji (Human Burkitt's lymphoma cell line) cells labeling CD40 using ab252557 at a 1/250 dilution in ICC.

Secondary : HRP-conjugated goat anti-rabbit IgG.

Substrate : DAB.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD40 antibody [BLR056F] (AB252557)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD40 antibody [BLR056F] (AB252557)

Formalin-fixed, paraffin-embedded human breast carcinoma tissue stained for CD40 with ab252557 at a 1/250 dilution in immunohistochemical analysis. DAB staining. HRP-conjugated goat anti-rabbit IgG secondary.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD40 antibody [BLR056F] (AB252557)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD40 antibody [BLR056F] (AB252557)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human breast carcinoma tissue stained for CD40 with ab252557 at a 1/250 dilution, followed by HRP-conjugated goat anti-rabbit IgG secondary. Substrate : Opal. Counterstain : DAPI (blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD40 antibody [BLR056F] (AB252557)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD40 antibody [BLR056F] (AB252557)

Formalin-fixed, paraffin-embedded human tonsil tissue stained for CD40 with ab252557 at a 1/250 dilution in immunohistochemical analysis. DAB staining. HRP-conjugated goat anti-rabbit IgG secondary.

Immunoprecipitation - Anti-CD40 antibody [BLR056F] (AB252557)
  • IP

Supplier Data

Immunoprecipitation - Anti-CD40 antibody [BLR056F] (AB252557)

CD40 was immunoprecipitated from Raji (Human Burkitt's lymphoma cell line) whole cell lysate (1 mg per IP reaction; 20% of IP loaded) prepared using NETN lysis buffer.
ab252557 used for IP at 5 μl per reaction. For WB 1/1000 dilution.

Lane 1 : ab252557 IP in Raji whole cell lysate.
Lane 2 : Control IgG IP in Raji whole cell lysate.

Chemiluminescence detection : 10 seconds.

All lanes:

Immunoprecipitation - Anti-CD40 antibody [BLR056F] (ab252557)

Predicted band size: 30 kDa

false

Western blot - Anti-CD40 antibody [BLR056F] (AB252557)
  • WB

Supplier Data

Western blot - Anti-CD40 antibody [BLR056F] (AB252557)

Lysates prepared using NETN lysis buffer.

All lanes:

Western blot - Anti-CD40 antibody [BLR056F] (ab252557) at 1/1000 dilution

Lane 1:

Raji (Human Burkitt's lymphoma cell line) whole cell lysate at 50 µg

Lane 2:

HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 3:

U-2 OS (Human bone osteosarcoma epithelial cell line) whole cell lysate at 50 µg

Lane 4:

GaMG (Human cell line) whole cell lysate at 50 µg

Lane 5:

Daudi (Human Burkitt's lymphoma cell line) whole cell lysate at 50 µg

Lane 6:

A549 (Human lung carcinoma cell line) whole cell lysate at 50 µg

Lane 7:

Ramos (Human Burkitt's lymphoma cell line) whole cell lysate at 50 µg

Lane 8:

HDLM2 (Human cell line) whole cell lysate at 50 µg

Lane 9:

RKO (Human cell line) whole cell lysate at 50 µg

Lane 10:

Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

Lane 11:

MJ (Human cell line) whole cell lysate at 50 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG

Predicted band size: 30 kDa

false

Exposure time: 10s

関連する標識済み抗体及び組成の異なる製品 (1)

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

BLR056F

アイソタイプ

IgG

キャリアフリー

No

交差種

Human

アプリケーション

WB, IHC-P, ICC, IP

applications

免疫原

Synthetic Peptide within Human CD40 aa 200-300. The exact immunogen used to generate this antibody is proprietary information.

P25942

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代替製品

Primary Antibodies

AB269301

FITC Anti-CD40 antibody [HI40a]

Flow Cytometry - FITC Anti-CD40 antibody [HI40a] (AB269301)

  • 0
  • 0
Primary Antibodies

AB271995

Anti-CD40 antibody [EPR20540] - BSA and Azide free

RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD40 antibody [EPR20540] - BSA and Azide free (AB271995)

  • 0
  • 0

組合せ製品

Assay Kits

AB99990

Human CD40 ELISA Kit

Sandwich ELISA - Human CD40 ELISA Kit (AB99990)

  • 0
  • 0
Secondary Antibodies

AB205718

Goat Anti-Rabbit IgG H&L (HRP)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP) (AB205718)

  • 5
  • 8
Primary Antibodies

AB92547

Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker

BOND RX™ Validated|KO Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size|Advanced Validation

Multiplex immunohistochemistry - Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (AB92547)

  • 5
  • 92
Primary Antibodies

AB40772

Anti-E Cadherin antibody [EP700Y] - Intercellular Junction Marker

BOND RX™ Validated|KO Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size|Advanced Validation

Western blot - Anti-E Cadherin antibody [EP700Y] - Intercellular Junction Marker (AB40772)

  • 4
  • 23
Primary Antibodies

AB24982

Cy5 ® Anti-CD45.1 antibody [A20]

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p>5 μl/1 mg lysate</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" } } }
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製品の詳細

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

This product is sold under License from Bethyl Laboratories, Inc.

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出荷温度及び保存条件

製品の状態
Liquid
精製に関する特記事項
Purified from cell culture supernatant.
バッファー組成
pH: 7.8 - 8.6 Preservative: 0.09% Sodium azide Constituents: 99% Borate buffered saline, 0.1% BSA
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
+4°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Receptor for TNFSF5/CD40LG (PubMed : 31331973). Transduces TRAF6- and MAP3K8-mediated signals that activate ERK in macrophages and B cells, leading to induction of immunoglobulin secretion (By similarity).
See full target information CD40
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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in pharmacology 16:1508047 PubMed40110131

2025

Amentoflavone protects against cisplatin-induced acute kidney injury by modulating Nrf2-mediated oxidative stress and ferroptosis and partially by activating Nrf2-dependent PANoptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Zhang,Jianqiang Hu,Yanmin Zhang,Xinxin Ci

Open medicine (Warsaw, Poland) 19:20241007 PubMed39308922

2024

Decreased BIRC5-206 promotes epithelial-mesenchymal transition in nasopharyngeal carcinoma through sponging miR-145-5p.

Applications

Unspecified application

Species

Unspecified reactive species

Weihua Xu,Junjie Hu,Zhichao Ma,Wanyi Feng,Wei Gong,Shengmiao Fu,Xinping Chen
View all publications
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