Anti-CD40 抗体 (ab58391)
Key features and details
- Rabbit polyclonal to CD40
- Suitable for: WB, IHC-P
- Reacts with: Human, African green monkey
- Isotype: IgG
製品の概要
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製品名
Anti-CD40 antibody
CD40 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to CD40 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human, African green monkey
交差が予測される動物種: Mouse
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免疫原
Synthetic peptide derived from the C-terminal of CD40 (Human)
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
Without Mg+2 and Ca+2 -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
Purified from rabbit antiserum. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab58391の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (1) |
1/500 - 1/1000. Detects a band of approximately 31 kDa (predicted molecular weight: 31 kDa).
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| IHC-P |
Use at an assay dependent concentration.
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| 特記事項 |
|---|
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WB
1/500 - 1/1000. Detects a band of approximately 31 kDa (predicted molecular weight: 31 kDa). |
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IHC-P
Use at an assay dependent concentration. |
ターゲット情報
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機能
Receptor for TNFSF5/CD40LG. -
組織特異性
B-cells and in primary carcinomas. -
関連疾患
Defects in CD40 are the cause of hyper-IgM immunodeficiency syndrome type 3 (HIGM3) [MIM:606843]; also known as hyper-IgM syndrome 3. HIGM3 is an autosomal recessive disorder which includes an inability of B cells to undergo isotype switching, one of the final differentiation steps in the humoral immune system, an inability to mount an antibody-specific immune response, and a lack of germinal center formation. -
配列類似性
Contains 4 TNFR-Cys repeats. -
細胞内局在
Secreted and Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 958 Human
- Entrez Gene: 21939 Mouse
- Omim: 109535 Human
- SwissProt: P25942 Human
- SwissProt: P27512 Mouse
- Unigene: 472860 Human
- Unigene: 271833 Mouse
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別名
- AI326936 antibody
- B cell associated molecule CD40 antibody
- B cell surface antigen CD40 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD40 antibody (ab58391)ab58391, at 1/50 diltuion, staining human CD40 in breast carcinoma tissue, by immunohistochemistry, in the presence or absence of the immunizing peptide. Paraffin embedded tissue.
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Western blot - Anti-CD40 antibody (ab58391)All lanes : Anti-CD40 antibody (ab58391) at 1/500 dilution
Lane 1 : COS7 cell extract with no immunizing peptide
Lane 2 : COS7 cell extract with immunizing peptide
Predicted band size: 31 kDa
Observed band size: 31 kDa
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (5)
ab58391 は 5 報の論文で使用されています。
- Zhou T et al. Microglia Polarization with M1/M2 Phenotype Changes in rd1 Mouse Model of Retinal Degeneration. Front Neuroanat 11:77 (2017). IHC ; Mouse . PubMed: 28928639
- Doublier S et al. Soluble CD40 ligand directly alters glomerular permeability and may act as a circulating permeability factor in FSGS. PLoS One 12:e0188045 (2017). PubMed: 29155846
- Cao DH et al. Pathological characteristics of CD40/CD40L and NF-?B proteins related to inflammation in benign prostatic hyperplasia tissue: a retrospective study of 120 cases. Int J Clin Exp Pathol 10:10863-10872 (2017). PubMed: 31966429
- Cowan RW et al. T cells stimulate catabolic gene expression by the stromal cells from giant cell tumor of bone. Biochem Biophys Res Commun 419:719-23 (2012). IHC . PubMed: 22386994
- Manczak M et al. Neutralization of granulocyte macrophage colony-stimulating factor decreases amyloid beta 1-42 and suppresses microglial activity in a transgenic mouse model of Alzheimer's disease. Hum Mol Genet 18:3876-93 (2009). IHC-Fr ; Mouse . PubMed: 19617638