Anti-CD4 抗体 [EPR19514] (ab183685)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19514] to CD4
- Suitable for: IP, IHC-P, IHC-Fr, WB
- Reacts with: Mouse
Related conjugates and formulations
製品の概要
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製品名
Anti-CD4 antibody [EPR19514]
CD4 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR19514] to CD4 -
由来種
Rabbit -
アプリケーション
適用あり: IP, IHC-P, IHC-Fr, WBmore details -
種交差性
交差種: Mouse -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: Mouse thymus, liver, brain and kidney tissue lysates; Raw264.7 and NIH/3T3 cell lysates IHC-P: Mouse spleen, colon, brain, cerebrum, lung and thymus tissues; mammary tumor tissue IHC-Fr: Mouse spleen and liver tissue. IP: Mouse thymus whole cell lysate.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR19514 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab183685の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IP |
1/40.
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IHC-P | (14) |
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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IHC-Fr | (4) |
1/200.
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WB | (1) |
1/1000. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa).
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特記事項 |
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IP
1/40. |
IHC-P
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
IHC-Fr
1/200. |
WB
1/1000. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa). |
ターゲット情報
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機能
Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts. -
配列類似性
Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
翻訳後修飾
Palmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts. -
細胞内局在
Cell membrane. Localizes to lipid rafts. Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum. - Information by UniProt
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参照データベース
- Entrez Gene: 12504 Mouse
- SwissProt: P06332 Mouse
- Unigene: 2209 Mouse
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別名
- CD 4 antibody
- CD4 (L3T4) antibody
- CD4 antibody
see all
画像
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Formaldehyde-fixed, non-permeabilized mouse colon tissue stained for CD4 with ab183685 (30 mins at a 1/500 dilution) in immunohistochemical analysis. A Rabbit polyclonal HRP conjugate was used as the secondary.
Heat mediated antigen retrieval buffer/enzyme used: pH 9.0 EDTA.
Blocking step: 1% ab64226 for 10 mins at RT.
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All lanes : Anti-CD4 antibody [EPR19514] (ab183685) at 1/2000 dilution
Lane 1 : Mouse thymus lysate
Lane 2 : Mouse liver tissue lysate
Lane 3 : Mouse brain tissue lysate
Lane 4 : Mouse kidney tissue lysate
Lane 5 : Raw264.7 (mouse abelson murine leukemia virus-induced tumor) whole cell lysate
Lane 6 : NIH/3T3 (mouse embryo) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 51 kDa
Observed band size: 51 kDaThe expression profile observed is consistent with what has been described in the literature (PMID: 2155425).
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1:3 seconds; Lanes 2,3,4,5 and 6:30 seconds
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IHC image of CD4 staining in a section of frozen normal Mouse Spleen.
The section was fixed using 10% formaldehyde in 1XPBS for 10 minutes. No antigen retrieval step was performed prior to staining. Non-specific protein-protein interactions were then blocked in TBS containing 0.025% (v/v) Triton X-100, 0.3M glycine and 1% (w/v) BSA for 1h at room temperature. The section was then incubated overnight at +4°C in TBS containing 0.025% (v/v) Triton X-100 and 1% (w/v) BSA with ab183685 (Rabbit monoclonal [EPR19514] to CD4) at 1/200 and ab243840 at 1µg/ml, to show the distinct staining of B cells and T cells. The section was then incubated with ab150165 (Goat Anti-Rat IgG H&L (Alexa Fluor® 488) preabsorbed, (Shown in green) 1/1000) and ab150080 (Goat Anti-Rabbit IgG H&L (Alexa Fluor®594) (Shown in red) 1/1000) for 1 hour at room temperature. The secondary-only control insert image is taken from an identical assay without primary antibody. DAPI was used to stain the cell nuclei (blue). The section was then mounted using Fluoromount®.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
For IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antibody concentrations and incubation times.
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Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen Mouse spleen tissue labeling CD4 with ab183685 at 1/500 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).
The result showed membrane staining on mouse spleen.
The nuclear counterstain is DAPI (blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 at 1/1000 dilution.
Antigen retrieval: Heated citrate solution (10mM citrate pH 6.0 + 0.05% Tween-20).
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Paraformaldehyde-fixed, 0.1% Tween 0.3% Triton in PBS permeabilized mouse liver tissue stained for CD4 with ab183685 (12 hours, 4°C at a 1/100 dilution) in immunohistochemical analysis. A Donkey anti Rabbit IgG (H+L) AlexaFluor®647 was used as the secondary at a 1/500 dilution (red).
Blocking step: 1% BSA for 12 hours at 4°C.
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Formalin-fixed, 0.2% Triton-X permeabilized mouse Eo771 mammary tumor tissue stained for CD4 with ab183685 (18 hours, 4°C at a 1/1000 dilution) in immunohistochemical analysis. A Goat anti Rabbit IgG (H&L) polyclonal AlexaFluor®488 conjugate was used as the secondary (green).
Heat mediated antigen retrieval buffer/enzyme used: Citrate buffer.
Blocking step: 4% BSA for 1 hour at 25°C.
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Formaldehyde-fixed, non-permeabilized mouse brain tissue stained for CD4 with ab183685 (14 hours, 4°C at a 1/1000 dilution) in immunohistochemical analysis. A Goat anti Rabbit IgG monoclonal HRP conjugate was used as the secondary at a 1/200 dilution.
Blocking step: 5% normal goat serum for 1 hour at 26°C.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Formaldehyde-fixed, paraffin-embedded mouse lung tissue stained for CD4 with ab183685 (18 hours, 4°C at a 1/1000 dilution) in immunohistochemical analysis. A Goat anti Rabbit IgG polyclonal HRP conjugate was used as the secondary at a 1/250 dilution (red).
Heat mediated antigen retrieval buffer/enzyme used: Citrate buffer pH 6.0.
Blocking step: 10% serum for 1 hour at 25°C.
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Formaldehyde-fixed, 3% hydrogen peroxide permeabilized B16F10 (melanoma) syngeneic mouse tumor tissue stained for CD4 with ab183685 (36mins, 37°C at a 1/1000 dilution) in immunohistochemical analysis. A Goat anti Rabbit IgG polyclonal HRP conjugate was used as the secondary.
Heat mediated antigen retrieval buffer/enzyme used: Tris/EDTA.
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Formaldehyde-fixed, non-permeabilized mouse spleen tissue stained for CD4 with ab183685 (30mins at a 1/1000 dilution) in immunohistochemical analysis. A Goat polyclonal HRP conjugate was used as the secondary.
Heat mediated antigen retrieval buffer/enzyme used: Leica ER2 solution.
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Formaldehyde-fixed mouse colon, chronic mucosal inflammation tissue stained for CD4 with ab183685 (90 mins at a 1/1000 dilution) in immunohistochemical analysis. A Goat polyclonal biotin conjugate was used as the secondary.
Heat mediated antigen retrieval buffer/enzyme used: High pH CC1.
Blocking step: 1% BSA for 40 mins at 24°C.
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CD4 was immunoprecipitated from 1mg of Mouse thymus whole cell lysate with ab183685 at 1/40 dilution.
Western blot was performed from the immunoprecipitate using ab183685 at 1/1000 dilution.
VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
Lane 1: Mouse thymus whole cell lysate, 10µg (Input).
Lane 2: ab183685 IP in Mouse thymus whole cell lysate.
Lane 3: Rabbit IgG,monoclonal [EPR25A] - Isotype Control (ab172730) instead of ab183685 in Mouse thymus whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 5 seconds.
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10% NBF-fixed, paraffin-embedded mouse spleen tissue stained for CD4 using ab183685 at 1/2000 dilution in immunohistochemical analysis, followed by Goat anti-Rabbit IgG Alexa Fluor® 647.
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Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling CD4 with ab183685 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Negative on mouse cerebrum.
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Formaldehyde-fixed, paraffin-embedded mouse spleen tissue stained for CD4 using ab183685 at 1/2000 dilution in immunohistochemical analysis, followed by Goat anti Rabbit IgG Biotin.
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Formaldehyde-fixed, paraffin-embedded mouse thymus tissue stained for CD4 using ab183685 at 1/500 dilution in immunohistochemical analysis.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (351)
ab183685 は 351 報の論文で使用されています。
- Rubio T et al. Beneficial Effect of Fingolimod in a Lafora Disease Mouse Model by Preventing Reactive Astrogliosis-Derived Neuroinflammation and Brain Infiltration of T-lymphocytes. Mol Neurobiol 61:3105-3120 (2024). PubMed: 37971656
- Caswell DR et al. The role of APOBEC3B in lung tumor evolution and targeted cancer therapy resistance. Nat Genet 56:60-73 (2024). PubMed: 38049664
- Jiang D et al. Euphorbia factor L2 suppresses the generation of liver metastatic ascites in breast cancer via inhibiting NLRP3 inflammasome activation. Int J Mol Med 53:N/A (2024). PubMed: 38063231
- Huang Z et al. Therapeutic Effect of Costunolide in Autoimmune Hepatitis: Network Pharmacology and Experimental Validation. Pharmaceuticals (Basel) 16:N/A (2023). PubMed: 37163367
- Shen MH et al. Propolis Has an Anticancer Effect on Early Stage Colorectal Cancer by Affecting Epithelial Differentiation and Gut Immunity in the Tumor Microenvironment. Nutrients 15:N/A (2023). PubMed: 37960147