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Anti-CD36 抗体 [FA6-152] (ab17044)

Reviews (2)Q&A (21)References (47)
Immunohistochemistry (Frozen sections) - Anti-CD36 antibody [FA6-152] (ab17044)
  • Flow Cytometry - Anti-CD36 antibody [FA6-152] (ab17044)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD36 antibody [FA6-152] (ab17044)
  • Flow Cytometry - Anti-CD36 antibody [FA6-152] (ab17044)

Key features and details

  • Mouse monoclonal [FA6-152] to CD36
  • Suitable for: IHC-P, Flow Cyt, IHC-Fr
  • Reacts with: Human
  • Isotype: IgG1

製品の概要

  • 製品名

    Anti-CD36 antibody [FA6-152]
    CD36 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [FA6-152] to CD36

  • 由来種

    Mouse

  • アプリケーション

    適用あり: IHC-P, Flow Cyt, IHC-Frmore details
    適用なし: WB

  • 種交差性

    交差種: Human

  • 免疫原

    Tissue, cells or virus corresponding to Human CD36. 20-Weeks-old fetal erythrocytes

  • ポジティブ･コントロール

    • Flow cytometry: THP-1 cells; IHC-Fr: Human tonsil tissue; IHC-P: Human breast carcinoma tissue.

  • 特記事項

    We have data from customer abReviews and publications indicating that this antibody is capable of detecting CD36 via western blot. However, based on customer feedback of difficulties and extensive troubleshooting using this antibody in western blot, we are removing WB as a tested application and recommend ab133625 as an alternative for use in this application.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab17044の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-P
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IHC-Fr (1)
Use at an assay dependent concentration.
特記事項
IHC-P
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IHC-Fr
Use at an assay dependent concentration.
追加情報
Is unsuitable for WB.

ターゲット情報

  • 機能

    Multifunctional glycoprotein that acts as receptor for a broad range of ligands. Ligands can be of proteinaceous nature like thrombospondin, fibronectin, collagen or amyloid-beta as well as of lipidic nature such as oxidized low-density lipoprotein (oxLDL), anionic phospholipids, long-chain fatty acids and bacterial diacylated lipopeptides. They are generally multivalent and can therefore engage multiple receptors simultaneously, the resulting formation of CD36 clusters initiates signal transduction and internalization of receptor-ligand complexes. The dependency on coreceptor signaling is strongly ligand specific. Cellular responses to these ligands are involved in angiogenesis, inflammatory response, fatty acid metabolism, taste and dietary fat processing in the intestine (Probable). Binds long-chain fatty acids and facilitates their transport into cells, thus participating in muscle lipid utilization, adipose energy storage, and gut fat absorption (By similarity) (PubMed:18353783, PubMed:21610069). In the small intestine, plays a role in proximal absorption of dietary fatty acid and cholesterol for optimal chylomicron formation, possibly through the activation of MAPK1/3 (ERK1/2) signaling pathway (By similarity) (PubMed:18753675). Involved in oral fat perception and preferences (PubMed:22240721, PubMed:25822988). Detection into the tongue of long-chain fatty acids leads to a rapid and sustained rise in flux and protein content of pancreatobiliary secretions (By similarity). In taste receptor cells, mediates the induction of an increase in intracellulare calcium levels by long-chain fatty acids, leading to the activation of the gustatory neurons in the nucleus of the solitary tract (By similarity). Important factor in both ventromedial hypothalamus neuronal sensing of long-chain fatty acid and the regulation of energy and glucose homeostasis (By similarity). Receptor for thombospondins, THBS1 and THBS2, mediating their antiangiogenic effects (By similarity). As a coreceptor for TLR4:TLR6 heterodimer, promotes inflammation in monocytes/macrophages. Upon ligand binding, such as oxLDL or amyloid-beta 42, interacts with the heterodimer TLR4:TLR6, the complex is internalized and triggers inflammatory response, leading to NF-kappa-B-dependent production of CXCL1, CXCL2 and CCL9 cytokines, via MYD88 signaling pathway, and CCL5 cytokine, via TICAM1 signaling pathway, as well as IL1B secretion, through the priming and activation of the NLRP3 inflammasome (By similarity) (PubMed:20037584). Selective and nonredundant sensor of microbial diacylated lipopeptide that signal via TLR2:TLR6 heterodimer, this cluster triggers signaling from the cell surface, leading to the NF-kappa-B-dependent production of TNF, via MYD88 signaling pathway and subsequently is targeted to the Golgi in a lipid-raft dependent pathway (By similarity) (PubMed:16880211).
    (Microbial infection) Directly mediates cytoadherence of Plasmodium falciparum parasitized erythrocytes and the internalization of particles independently of TLR signaling.

  • 関連疾患

    Platelet glycoprotein IV deficiency
    Coronary heart disease 7

  • 配列類似性

    Belongs to the CD36 family.

  • 翻訳後修飾

    N-glycosylated and O-glycosylated with a ratio of 2:1.
    Ubiquitinated at Lys-469 and Lys-472. Ubiquitination is induced by fatty acids such as oleic acid and leads to degradation by the proteasome (PubMed:21610069, PubMed:18353783). Ubiquitination and degradation are inhibited by insulin which blocks the effect of fatty acids (PubMed:18353783).

  • 細胞内局在

    Cell membrane. Membrane raft. Golgi apparatus. Apical cell membrane. Upon ligand-binding, internalized through dynamin-dependent endocytosis.
  • Information by UniProt

  • 参照データベース

    • 別名

      • Adipocyte membrane protein antibody
      • BDPLT10 antibody
      • CD36 antibody
      • CD36 antigen (collagen type I receptor, thrombospondin receptor) antibody
      • CD36 antigen antibody
      • CD36 molecule (thrombospondin receptor) antibody
      • CD36 molecule antibody
      • CD36_HUMAN antibody
      • CHDS7 antibody
      • Cluster determinant 36 antibody
      • Collagen receptor, platelet antibody
      • FAT antibody
      • Fatty acid translocase antibody
      • Fatty acid transport protein antibody
      • Glycoprotein IIIb antibody
      • GP IIIb antibody
      • GP3B antibody
      • GP4 antibody
      • GPIIIB antibody
      • GPIV antibody
      • Leukocyte differentiation antigen CD36 antibody
      • MGC108510 antibody
      • MGC91634 antibody
      • PAS 4 protein antibody
      • PAS IV antibody
      • PAS-4 antibody
      • PASIV antibody
      • Platelet collagen receptor antibody
      • Platelet glycoprotein 4 antibody
      • Platelet glycoprotein IV antibody
      • scarb3 antibody
      • Scavenger receptor class B member 3 antibody
      • SR-B3 antibody
      • SRB3 antibody
      • Thrombospondin receptor antibody
      see all

    画像

    • Immunohistochemistry (Frozen sections) - Anti-CD36 antibody [FA6-152] (ab17044)
      Immunohistochemistry (Frozen sections) - Anti-CD36 antibody [FA6-152] (ab17044)

      Immunohistochemistry of frozen section of human tonsil tissue stained for CD36 with ab17044 at 20 µg/ml.

    • Flow Cytometry - Anti-CD36 antibody [FA6-152] (ab17044)
      Flow Cytometry - Anti-CD36 antibody [FA6-152] (ab17044)

      Detection of CD36 in THP-1 cells. Red, black and blue line represent the isotype control, cells only and ab17044 at 10 μg/ml, respectively.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD36 antibody [FA6-152] (ab17044)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD36 antibody [FA6-152] (ab17044)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of human breast carcinoma stained for CD36 with ab17044 at 20 µg/ml

    • Flow Cytometry - Anti-CD36 antibody [FA6-152] (ab17044)
      Flow Cytometry - Anti-CD36 antibody [FA6-152] (ab17044)
      Overlay histogram showing THP1 cells stained with ab17044 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab17044, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in 80% methanol (5 min) fixed THP1 cells used under the same conditions.

      Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

    参考文献 (47)

    ab17044 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab17044 は 47 報の論文で使用されています。

    • Gázquez A  et al. Calcifediol During Pregnancy Improves Maternal and Fetal Availability of Vitamin D Compared to Vitamin D3 in Rats and Modifies Fetal Metabolism. Front Nutr 9:871632 (2022). PubMed: 35495908
    • Meçe O  et al. Lipid droplet degradation by autophagy connects mitochondria metabolism to Prox1-driven expression of lymphatic genes and lymphangiogenesis. Nat Commun 13:2760 (2022). PubMed: 35589749
    • Edler MK  et al. Age, sex, and regional differences in scavenger receptor CD36 in the mouse brain: Potential relevance to cerebral amyloid angiopathy and Alzheimer's disease. J Comp Neurol 529:2209-2226 (2021). PubMed: 33319367
    • Yusuf B  et al. The neurorepellent, Slit2, prevents macrophage lipid loading by inhibiting CD36-dependent binding and internalization of oxidized low-density lipoprotein. Sci Rep 11:3614 (2021). PubMed: 33574432
    • Liang F  et al. Erythropoietin Promotes Infection Resolution and Lowers Antibiotic Requirements in E. coli- and S. aureus-Initiated Infections. Front Immunol 12:658715 (2021). PubMed: 33927725
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-10 of 23 Abreviews or Q&A

    Blocking abreview for Anti-CD36 antibody [FA6-152]

     Poor
    Abreviews
    abreview image
    Application
    Blocking
    Sample
    Mouse Cell (mouse macrophage cell line(raw264.7 cell))
    Specification
    mouse macrophage cell line(raw264.7 cell)
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Nov 27 2014

    Question

    Dear Abcam



    I have previously used a CD36 antibody from you (ab36977) with success but have noticed that it unfortunately no longer is available. Instead we tried ab78054 (with major problems, which we had a mail correspondence with you about in November 2011) and now we have tried the ab17044 but unfortunately also with problems.



    The homogenate used for testing the ab17044 has also been used for the other two antibodies :

    Human skeletal muscle tissue homogenized in Tris buffer (25mM Tris, 5mM EDTA, 3% SDS including Protease and phosphatase inhibitors: 20mM β-glycerophosphate, 10mM pyrophosphate,2mM NaOrtovanadate, 2.5mM PMSF, 1 mini comple protease inhibitor tablet (Roche) to 10ml buffer.)

    For Antibody ab36977 and ab78054 I also used a homogenate based on the RIPA buffer according to your suggestions. As you can see below in the previous mail, there is basically no difference between the two homogenates, why I only used the Tris-buffer based for this testing as most of our homogenates are based on that buffer.



    My problems with the ab17044 is that it gives several bands all very high in the gel. You have previously written to me:

    “Regarding the multiple bands with ab36977. This protein undergoes modifications such as glycosylation and palmitoylation which can certainly shift the bands you observe to ˜75 - 80kDa. The ˜50kDa band is likely unmodified protein”

    Which make sense, but now the bands are situated even higher in the gel:

    I am unsure whether the very intense band above 100kDa are specific?? If I block the signal (by laying something over the membrane when the picture is taken) I get this result, which actually is quite nice, but have I by that removed some CD36 signal? The middle part of the membrane was used for testing another antibody without success.

    CD 36 on Lane A-D: all with 15µg total protein.

    Lane A:

    Blocking for 1½ hour in 5% BSA in TBST at RT

    overnight incubation at 4 degrees with antibody diluted in 5% BSA in TBST with agitation (in a tube rolling) with a 1:500 dilution of ab17044.



    Lane B:

    Blocking for 1½ hour in 5% BSA in TBST at RT

    overnight incubation at 4 degrees with antibody diluted in 5% BSA in TBST with agitation (in a tube rolling) with a 1:1000 dilution of ab17044.



    Lane C:

    Blocking for 1½ hour in 3% BSA in TBST at RT

    overnight incubation at 4 degrees with antibody diluted in 3% BSA in TBST with agitation (in a tube rolling) with a 1:500 dilution of ab17044.



    Lane D:

    Blocking for 1½ hour in 3% BSA in TBST at RT

    overnight incubation at 4 degrees with antibody diluted in 3% BSA in TBST with agitation (in a tube rolling) with a 1:1000 dilution of ab17044.



    All lanes

    Sec. antibody was diluted in 5% BSA in TBST and incubated for 2 hours at 25 degrees in a tube rolling.



    All wash steps was in TBST. Both membranes was incubated with ECL and picture was taken with a CCD camera in 1 min.



    How many of the bands are specific antibody reactions with CD36 proteins??



    Kind regards

    Read More
    Answer

    Thank you for taking the time to send us your results. I am sorry to hear you have had difficulty obtaining satisfactory results from this CD36 antibody as well.

    The details you have kindly provided will enable us to investigate this case for you and this is also helpful in our records for monitoring of quality.

    The bands you observe above 100 kDa are likely to be unspecific. Reviewing this case, it seems your sample has not migrated sufficiently into the gel which would also explain that your testing for another antibody failed.

    I would like to offer some suggestions to help optimise the results you obtained from ab17044. I would also appreciate if you can confirm some further details:

    1) Your target is amembrane protein and it is heavily modified (as discussed before), and these proteins have the unfortunate tendency to aggregate during boiling periods with high temperatures and beta- mercaptoethanol (bME) due to their hydrophobic nature. I would like to suggest to use DTT as reducing agent rather than bME and to decrease the boiling temperature to 65ºC and to prolong the boiling time up to 10 min.

    2) What gel percentage have you used and which buffer system? For this target 8-10% gels might be sufficient. Also, the buffer system can have an influence thesize at which a bandruns (see attached).

    3) To verify the transfer of the proteins to the membrane I would recommend the Ponceau staining, if you have not already tried (see protocol details attached).

    I would hope that these suggestions help to optimise the results and would lead to distinct bands for CD36. It would then not be necessary to 'block out' the unspecific signals (which is also not really common scientific practise). Usually, the specific signal of an antibody is blocked by incubating the antibodywith the immunogenic peptide prior to staining the membrane. This way the specific signal disappears whereas unspecific background staining would still be present, validating the results. Unfortunately, the exact epitope of this particular CD36 antibody is unknown, and therefore, this blocking of the signal is not an option.

    I hope this information is helpful for you though, thank you for your cooperation. Should the suggestions not improve the results, please do not hesitate to contact me again with the further requested details.

    Read More

    Question

    Dear Sir
    The customer has the problem about the storage instruction for ab17044, He storage at 4C,do not freeze which follow abcam instruction but new instruction it change are store at -20°C. Avoid freeze / thaw cycles. He has doubt that for this reason it don't get result?
    Please kindly advise.
    Best regards

    Read More
    Answer

    Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this our Anti-CD36 antibody [FA6-152] (ab17044) .

    The details you have kindly provided will provide us with vital information for our monitoring of product quality

    I appreciate the time you have spent in the laboratory and understand your concerns. It is regrettable the results have not been successful. Reviewing the details, I am sorry there are no further tips to provide on this occasion to help improve the results. I can confirm that the vial might not be working for you because of the circumstances.

    We discovered from our source that this antibody is stable at -20 degrees. Therefore we now recommend to store it at this temperature.

    I apologise for the inconvenience and am pleased to offer you a free of charge replacement of the same product, a credit note or refund in compensation.

    In addition, I can suggest you may like to consider the following suggestions as a check for future experiments:

    We recommend a positive control to confirm the quality of the primary antibody. For the Anti-CD36 antibody [FA6-152] (ab17044) we can recommend HEL cells.

    Furthermore we suggest to make sure that the secondary antibody is raised against the isotype of the primary antibody if not already done. Additionally it would be useful to confirm that the secondary antibody is working well with other primary antibodies.

    Thank you for your cooperation. I look forward to hearing from you with details of how you would like to proceed.

    Read More

    Question

    Inquiry: I want to know what the antigen recognition site of Anti-CD36 antibody [FA6-152] (ab17044)? is amino acid peptide sequences of CD36 or peptide modified by glycosylation( the antigenic determinantthe )? Thank you very much!

    Read More
    Answer

    Thank you for contacting us.

    The immunogen for ab17044 is 20 weeks old fetal erythrocytes. However, we have not further mapped the epitope for this antibody.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    Question

    WB with human endothelial cells (MVEC cells)
    lysis buffer: commercial
    sees no bands at 55 or 88 kDa (glycoslyated form)
    bands instead at 200 kDa and below 25 kDa (very strong bands)
    loaded 25-30 ul/well
    block: 5% BSA
    2nd: OK

    Read More
    Answer

    Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number xxx with ab64014.

    To check the status of the order please contact our Customer Service team and reference this number.

    Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

    I wish you the best of luck with your research.

    Read More

    Question

    ab128568 and ab17044 give no signal in WB on rat samples

    Read More
    Answer

    Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number 1135221.

    To check the status of the order please contact our Customer Service team and reference this number.

    Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

    I wish you the best of luck with your research.

    Read More

    Question

    Dear Abcam,

    Please find the attachment and find some solution

    Read More
    Answer

    Thank you for filling in the questionnaire. I am sorry to hear that you are still experiencing problems with this antibody.
    I have looked through your protocol and would like to ask you some questions in order to understand your procedure better as well as make a few suggestions to improve your results:
    - Could you please provide further details about the sample type used? What kind of sample homogenate was used, whole cell lysate, purified protein?
    - This particular protein shows different sites of glycosilation, producing bands at higher molecular weights, and so, sample preparation is crucial. Make sure the protein is running under denatured / reducing conditions.
    - Have you checked with other techniques the negative control does not contain this cell marker?
    - Have you run a non primary control to dismiss the non specific band corresponds to the secondary?
    It would be really helpful if you could provide us with some images from any of the western blots performed, to try to find out why the antibody did not perform as stated on the datasheet.
    Could you also please provide the order number? As our Abpromise states, in the event that a product is not functioning in the applications/species cited on the product data sheet (and the problem has been reported within 6 months of purchase) we will happily offer a credit note/refund to the value of the product purchased.
    I look forward to receiving your reply. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Question

    Do you have more details about the blocking ability of this antibody?

    Read More
    Answer

    Thank you for contacting us.

    According to our information, we have that platelet aggregation and secretion was induced by > 1μg/ml of this antibody, according to this reference: Kieffer N et al; Development regulated expression of a 78 kDa erythroblast membrane glycoprotein immunologically related to the platelet thrombospondin receptor. Biochem J 1989, 262: 835

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    Question

    I have done western blotting with this antibody. A prominent band was developed near 100 kD marker. Problem is, the same band is also present in negative control. How can we resolve this problem.

    Read More
    Answer

    Thank you for contacting us.
    I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.
    In order to better understand the problem, I would like to send you a questionnaire with some questions about the protocol used, as well as the order details. All this information is crucial to help us understand the possible causes of the problem. Any images are highly appreciated.
    Once we receive the completed questionnaire, we will look at the protocol and see if there are any suggestions we can make that may improve the results. If no suggestions can be made to the protocol, and the antibody was purchased within the last 6 months, it is covered by our Abpromise guarantee, and therefore I can offer you a free of charge replacement or a credit note for it.
    I look forward to receiving your reply. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Question

    Thanks for your information about mouse CD36 antibody. I want to detect CD36 from c2c12 cell lines, a mouse cell lines. I just request you if you send me a little aliquot 10 microlitre,I will just check 1 exp, then I will order. I will need a good amount to order. Please let me know it is possible or not.

    Read More
    Answer

    Thank you for contacting us. Because we carry over 70,000 products, it isn't feasible for us to keep small sample sizes of our products.

    We are happy to reassure our customers that all of our products are covered by our Abpromise, which guarantees that the product will work in the applications and species specified on the datasheet, or we will offer a replacement, credit, or refund within 6 months of purchase.

    If the product is to be used in an untested species or application, you may be eligible for our testing discount program if the antibody has not yet been purchased. Please contact our Scientific Support team by replying to this email prior to purchase for more information.

    Otherwise, we like to encourage all of our customers to submit an Abreview via the online product datasheet. We always appreciate customer feedback, whether positive or negative, and we make all product information available to researchers. Plus, each Abreview earns Abpoints that can be used for discounts on future purchases or rewards such as Amazon.com gift certificates.

    To find out more about our Abreview system, please see the following link:

    https://www.abcam.com/abreviews

    I hope this information is helpful. Please do not hesitate to contact us again with any other questions.

    Read More

    1-10 of 23 Abreviews or Q&A

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