Anti-CD34 抗体 [4H11(APG)] (ab762)
Key features and details
- Mouse monoclonal [4H11(APG)] to CD34
- Suitable for: Flow Cyt, IHC-P
- Reacts with: Human
- Isotype: IgG1
Related conjugates and formulations
製品の概要
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製品名
Anti-CD34 antibody [4H11(APG)]
CD34 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [4H11(APG)] to CD34 -
由来種
Mouse -
特異性
This antibody reacts with Class III epitope on CD34 antigen. -
アプリケーション
適用あり: Flow Cyt, IHC-Pmore details
適用なし: WB -
種交差性
交差種: Human -
免疫原
Tissue, cells or virus corresponding to Human CD34. Permanent human cell line derived from peripheral leucocytes of a patient suffering from chronic myeloid leukaemia
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ポジティブ・コントロール
- IHC-P: Human normal placenta. Flow Cytometry: human peripheral whole blood.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading...
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精製度
Protein A purified -
特記事項(精製)
Purified from TCS. Purity >95% by SDS-PAGE. -
ポリ/モノ
モノクローナル -
クローン名
4H11(APG) -
アイソタイプ
IgG1 -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab762の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Flow Cyt |
Use a concentration of 2 µg/ml.
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IHC-P |
Use a concentration of 10 µg/ml.
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特記事項 |
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Flow Cyt
Use a concentration of 2 µg/ml. |
IHC-P
Use a concentration of 10 µg/ml. |
ターゲット情報
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機能
Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins. -
組織特異性
Selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues. -
配列類似性
Belongs to the CD34 family. -
発生段階
On early hematopoietic progenitor cells. -
翻訳後修飾
Highly glycosylated.
Phosphorylated on serine residues by PKC. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 947 Human
- Omim: 142230 Human
- SwissProt: P28906 Human
- Unigene: 374990 Human
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別名
- CD34 antibody
- CD34 antigen antibody
- CD34 molecule antibody
see all
画像
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Flow cytometric surface staining pattern showing CD34 positive stem cells in red stained with 2µg/ml ab762 in human peripheral whole blood.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [4H11(APG)] (ab762)
IHC image of CD34 staining in Human normal placenta formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab762, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (7)
ab762 は 7 報の論文で使用されています。
- Goyal D et al. Vascular Stem Cells and the Role of B-Raf Kinase in Survival, Proliferation, and Apoptosis. Int J Mol Sci 24:N/A (2023). PubMed: 37108645
- Shi F et al. Relationship between the expression of ARHGAP25 and RhoA in non-small cell lung cancer and vasculogenic mimicry. BMC Pulm Med 22:377 (2022). PubMed: 36207695
- Yang R et al. Low PRRX1 expression and high ZEB1 expression are significantly correlated with epithelial-mesenchymal transition and tumor angiogenesis in non-small cell lung cancer. Medicine (Baltimore) 100:e24472 (2021). PubMed: 33530259
- Cheng L et al. FOXM 1 induces Vasculogenic mimicry in esophageal cancer through ß-catenin /Tcf4 signaling. Diagn Pathol 15:14 (2020). PubMed: 32035486
- Nies C et al. A Microcavity Array-Based 4D Cell Culture Platform. Bioengineering (Basel) 6:N/A (2019). PubMed: 31159244
- Cheng L et al. Aberrant expression of MYH9 and E-cadherin in esophageal squamous cell carcinoma and their relationship to vasculogenic mimicry. Int J Clin Exp Pathol 12:2205-2214 (2019). PubMed: 31934043
- Mattheij NJ et al. Dual mechanism of integrin aIIbß3 closure in procoagulant platelets. J Biol Chem 288:13325-36 (2013). PubMed: 23519467